Enhancement of sensitivity of SPR protein microarray using a novel 3D protein immobilization

Tanaka, Hiroyuki; Hanasaki, Minako; Isojima, Tatsushi; Takeuchi, Hisao; Shiroya, Toshifumi; Kawaguchi, Haruma

doi:10.1016/j.colsurfb.2008.12.037

Cited by 31 publications

(14 citation statements)

References 21 publications

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“…These parameters are greatly influenced by the topography of the biosensor support material (Welch et al, 2017), and the chemical immobilization technique used (Yu et al, 2015). A 3D biosensor substrate will allow a larger amount of immobilized biorecognition probes, since it offers increased surface area when compared with planar surfaces, which are usually associated with low immobilization efficiency (Gupta and Chaudhury, 2007;Tanaka et al, 2009;Wang and Feng, 2015). In addition, 3D material supports can also improve the maintenance the biological activity of protein probes by minimizing steric hindrance, providing improved probe orientation and stability for enhanced analyte capture.…”

Section: Probe Immobilizationmentioning

confidence: 99%

3D biosensors in advanced medical diagnostics of high mortality diseases

Rebelo

Barbosa

Caballero

et al. 2019

Biosensors and Bioelectronics

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Cardiovascular diseases, cancer, and diabetes are high mortality diseases, which account for almost two thirds of all deaths worldwide. Their early detection and continuous evaluation are fundamental for an improved patient prognosis and reduced socioeconomic impact. Current biosensor technologies are typically based on the analysis of whole blood samples from patients for the detection of disease-specific biomarkers. However, these technologies display serious shortcomings, such as reduced sensitivity and dynamic range, limited in vivo applicability, and lack of continuous monitoring. There is the urgent need for new diagnostic and treatment follow-up tools, which allow for the early detection of the pathology as well as for the continuous monitoring of the physiological responses to specific therapies. During the last years, a new generation of biosensor technologies with improved performance has emerged in the biomedical sector. The combination of advanced biomaterial methods, biochemical tools, and micro/nanotechnology approaches has resulted in the development of innovative three-dimensional (3D) biosensor platforms for advanced medical diagnosis. In this review, we report the most recent advances in the field of 3D biosensors for clinical applications, focusing on the diagnosis and monitoring of cardiovascular diseases, cancer, and diabetes. We discuss about their clinical performance compared to standard biosensor technologies, their implantable capability, and their integration into microfluidic devices to develop clinically-relevant models. Overall, we anticipate that 3D biosensors will drive us toward a new paradigm in medical diagnosis, resulting in real-time in vivo biosensors capable to significantly improve patient prognosis.

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Section: Probe Immobilizationmentioning

confidence: 99%

3D biosensors in advanced medical diagnostics of high mortality diseases

Rebelo

Barbosa

Caballero

et al. 2019

Biosensors and Bioelectronics

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“…Hydrogels can be used to assay a variety of biological targets, including endogenous proteins or peptides (as in traditional Peptidomics applications) (40,41), proteolytic peptides (as in Affinity Peptidomics) (8,10), synthetic peptides (e.g. for validation experiments), glycans or small-molecule ligands (42,43).…”

Section: Affinity Binding Assays On Hydrogels (See Note 57)mentioning

confidence: 99%

“…The hemolymph will keep flowing out of the crab during this step, but the super epoxy will gradually begin to harden, slowing the flow of hemolymph. 41. Try not to move the probe drastically, as you could run into some tissue on the sides of the pericardial cavity and clog the probe membrane.…”

mentioning

confidence: 99%

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Bioinformatic Approaches to the Identification of Novel Neuropeptide Precursors

Clynen

Liu

Husson

et al. 2009

Methods in Molecular Biology

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With the entire genome sequence of several animals now available, it is becoming possible to identify in silico all putative peptides and their precursors in an organism. In this chapter we describe a searching algorithm that can be used to scan the genome for predicted proteins with the structural hallmarks of (neuro)peptide precursors. We also describe how to use search strings such as the presence of a glycine residue as a putative amidation site, dibasic cleavage sites, the presence of a signal peptide, and specific peptide motifs to improve a standard BLAST search and make it suitable for searching (neuro)peptides in EST data. We briefly explain how bioinformatic tools and in silico predicted peptide precursor sequences can aid experimental peptide identification with mass spectrometry.

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“…Oriented immobilization of proteins on a solid surface is a fundamental interest in the study of the interaction between receptors and ligands [16][17][18][19]. The His-tag chemistry causes the correct orientation of the protein.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of His-tagged kinase JAK2 onto the surface of a plasmon resonance gold disc modified with different copper (II) complexes

Kurzątkowska¹,

Mielecki²,

Grzelak³

et al. 2014

Talanta

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New surface plasmon resonance (SPR) sensing platform swhich consists of copper (II) complexes of a pentetic acid thiol ligand (DPTA-Cu(II)) and of a thiol derivative of dipyrromethene (DPM-Cu(II) created on the surface of gold SPR disc were applied to oriented immobilization of His-tagged Janus kinase 2 (GST-His 6 -JAK2). This method is based on the covalent bond formation between histidine from a His-tag chain of a protein and Cu(II) centres from the complexes. The kinetic and thermodynamic parameters of the oriented immobilization of GST-His 6 -JAK2 protein to DPTA-Cu(II) and DPM-Cu(II) complexes attached to the Au surface of a SPR disc were discussed.

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Enhancement of sensitivity of SPR protein microarray using a novel 3D protein immobilization

Cited by 31 publications

References 21 publications

3D biosensors in advanced medical diagnostics of high mortality diseases

3D biosensors in advanced medical diagnostics of high mortality diseases

Bioinformatic Approaches to the Identification of Novel Neuropeptide Precursors

Immobilization of His-tagged kinase JAK2 onto the surface of a plasmon resonance gold disc modified with different copper (II) complexes

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