2009
DOI: 10.1080/02827580903228862
|View full text |Cite
|
Sign up to set email alerts
|

Enhancement of somatic embryogenesis from immature zygotic embryos of Pinus sylvestris

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1

Citation Types

2
42
1
3

Year Published

2013
2013
2022
2022

Publication Types

Select...
6
3

Relationship

1
8

Authors

Journals

citations
Cited by 50 publications
(53 citation statements)
references
References 19 publications
2
42
1
3
Order By: Relevance
“…The initiation of somatic embryogenesis in conifers is under strong genetic control as has been mentioned earlier in the text (Niskanen et al 2004). Depending on the family, in Pinus sylvestris the initiation frequencies varied from 0 to 26.7% (Aronen et al 2009). Similarly in Pinus armandii across nine open-pollinated families, initiation frequency ranged from 0 to 20% with an average of 1.5% (Maruyama et al 2007).…”
Section: Discussionmentioning
confidence: 94%
“…The initiation of somatic embryogenesis in conifers is under strong genetic control as has been mentioned earlier in the text (Niskanen et al 2004). Depending on the family, in Pinus sylvestris the initiation frequencies varied from 0 to 26.7% (Aronen et al 2009). Similarly in Pinus armandii across nine open-pollinated families, initiation frequency ranged from 0 to 20% with an average of 1.5% (Maruyama et al 2007).…”
Section: Discussionmentioning
confidence: 94%
“…2a). Using megagametophytes with immature embryos as explants is advantageous owing to the Immature embryos enclosed in megagametophytes have been used as explants in several conifer species as Abies alba (Gajdošová et al 1995;Krajňáková et al 2013a), Abies nordmanniana (Nörgaard and Krogstrup 1991), Juniperus communis (Helmersson and von Arnold 2009), Taxus brevifolia and T. cuspidata (Ewald et al 1995) but are typical for the species of the genus Pinus, as Pinus sylvestris (Aronen et al 2009), Pinus radiata (Montalbán et al 2012;Hargreaves et al 2009), Pinus pinaster (Humanez et al 2012), Pinus nigra (Salajova and Salaj 1992), Pinus palustris (Jones and van Staden 1995), Pinus halepensis (Montalbán et al 2013) Pinus oocarpa (Lara-Chavez et al 2011). Immature zygotic embryos excised from megagametophytes have been used in Picea glauca and Picea mariana (Hakman and Fowke 1987), Picea abies (Nagmani et al 1987), Picea sitchensis (Krogstrup et al1988).…”
Section: Initiation Of Somatic Embryogenesismentioning
confidence: 99%
“…Some examples of mature somatic embryo yield (number) calculated per 1 g of fresh weight: 64 for Abies fraseri (Kim et al 2009), 36 for Abies cephalonica (Krajňáková et al 2009), 187 in Pinus pinaster (Alvarez et al 2013), 321 in Pinus sylvestris (Aronen et al 2009), 269 for Picea glauca (Kong and Yeung 1995), 65 for Picea mariana (Tremblay and Tremblay 1995), 300-960 for hybrid of Larix (Lelu et al 1994). An undesirable phenomenon -loss of ability to produce mature somatic embryos -has been observed in embryogenic tissues of many species (Klimaszewska et al 2009).…”
Section: Maturation Of Somatic Embryosmentioning
confidence: 99%
“…Embryo maturation in pines is drastically affected by the gel strength of the culture medium, which determines water availability. In P. strobus, P. sylvestris and P. pinaster, reduced water availability (9-10 g L −1 gellan gum) results in the formation of cotyledonary se within 12 weeks, which are able to germinate (Klimaszewska and Smith 1997;Ramarosandratana et al 2001;Lelu-Walter et al 2006, 2008Aronen et al 2009), while increased water availability (4 g L −1 gellan gum) leads to the excessive proliferation of EM, thereby interfering with embryo maturation (Morel et al 2014a). In P. pinaster, significant differences between both conditions were detected at the molecular level after only 1 week of culture on the maturation medium (Morel et al 2014a).…”
Section: Somatic Embryo Development and Maturationmentioning
confidence: 96%