Enhancement of sperm quality and fertility-related parameters in Hubbard grandparent rooster fed diets supplemented with soybean lecithin and vitamin E

Abstract

The purpose of this study was to investigate the effect of dietary supplementation of different levels of soybean lecithin and vitamin E on semen quality parameters and some reproductive hormones in Hubbard grandparent roosters. The experiment was conducted in a 3 × 2 factorial arrangement with 3 levels of soybean lecithin (0, 1, and 2%) and 2 levels of vitamin E (0 and 300 mg/kg). Semen samples were collected on d 0, 20, 40 and 60 of the experiment and analyzed. Adding 1% soybean lecithin and vitamin E into the diet increased semen volume and sperm concentration, membrane integrity and viability ( P < 0.05). Supplementing diets with 1 or 2% lecithin in addition to vitamin E significantly improved total motility and progressive motility ( P < 0.05). Vitamin E significantly increased the amplitude of lateral head displacement ( ALH ) of sperm ( P < 0.05). Although there was no effect on LH and FSH when diets were supplemented with vitamin E and 1 or 2% lecithin, testosterone concentration was increased ( P < 0.05). Malondialdehyde ( MDA ) concentration was significantly lower in all 3 treatments containing vitamin E ( P < 0.05). It can be concluded that supplementation of rooster diets with vitamin E and 1% lecithin can improve fertility related parameters in Hubbard grandparent roosters.

Improving the quality of chilled semen from Thai native chicken using phosphorus and vitamin B12 supplementation in semen extender

“…Sperm characteristics including viability, plasma membrane integrity, and mitochondria activity are important factors contributing to sperm fertilization potential ( Blesbois and Brillard, 2007 ; Shabani et al, 2021 ). The fertility ability of rooster semen was evaluated to determine its quality.…”

Over 100 years of vitamin E: An overview from synthesis and formulation to application in animal nutrition

“…Shabani et al (2022) investigated the effect of dietary supplementation of different levels of soybean lecithin and vitamin E on semen quality parameters and some reproductive hormones in Hubbard grandparent roosters. Roosters were fed three levels of soybean lecithin, 0%, 1% and 2%, and two levels of vitamin E, 120 (basal diet) and 300 mg/kg feed.…”

Evaluation of Dry Ice for Short-Term Storage and Transportation of Frozen Boar Semen

“…Spermatozoa plasma membrane integrity was determined using the hypotonic swelling test (HOST). The tails of normal spermatozoa swelled and curved when exposed to HOS solution, while low-viability or dead spermatozoa did not undergo any significant swelling [15]. In brief, 10 µL semen samples was added to 100 µL of HOST solution (100 mOsm/L, 57.6 mM fructose, and 19.2 mM sodium citrate) at 37 • C for 30 min.…”