Enhancement of Submergence Tolerance in Transgenic Rice Overproducing Pyruvate Decarboxylase

Quimio, Celsa A.; Torrizo, Lina; Setter, T.L.; Ellis, Marc H.; Grover, Anil; Abrigo, E.; Oliva, Norman; Ella, Evangelina S.; Carpena, A. L.; Ito, Osamu; Peacock, W. J.; Dennis, Elizabeth S.; Dattal, Swapan K.

doi:10.1016/s0176-1617(00)80167-4

Cited by 91 publications

(44 citation statements)

References 20 publications

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“…Therefore, anaerobic pathway flux may play an important role in affecting reducing sugar accumulation, and consequently processing quality of potato tubers stored at low temperatures. The role of PDC in the anaerobic pathway and in stress response and tolerance has been widely reported (Drew 1997;Morrell et al 1990;Quimio et al 2000;Ismond et al 2003). We suggest a similar mechanism in our transgenic tubers responsible for LTS-tolerance.…”

Section: Discussionsupporting

confidence: 78%

“…Research results also support that enhancement of PDC levels in transgenic rice overexpressing PDC corresponded with an increase in submergence tolerance (Quimio et al 2000;Ismond et al 2003;Kursteiner et al 2003;Agarwal and Grover 2006). Recently, it has been reported that the increased hypoxia tolerance in Malus hupehensis may be partly due to the higher enzyme activity of PDC, ADH and LDH (Li et al 2010).…”

Section: Activity Of Pyruvate Decarboxylasesupporting

confidence: 70%

“…Ismond et al (2003) reported that an increased PDC activity resulted in increased carbon flow through the ethanol pathway as reflected by an increase in both acetaldehyde and ethanol pool sizes. Similarly, Quimio et al (2000) found that enhancement of PDC levels in transgenic rice overexpressing PDC corresponded with an increase in submergence tolerance. Biochemical tests have indicated that ADH does not limit fermentation but PDC has a fine control on fermentation pathway due to its low maximum catalytic capacity (Drew 1997;Morrell et al 1990).…”

Section: Introductionmentioning

confidence: 82%

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Alleviation of low temperature sweetening in potato by expressing Arabidopsis pyruvate decarboxylase gene and stress-inducible rd29A: A preliminary study

Pinhero

Pazhekattu

Marangoni

et al. 2011

Physiol Mol Biol Plants

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The acceptability of potatoes for processing chips and French fries is largely dependent on the color of the finished product. Most potato cultivars and varieties stored at temperatures below 9-10°C are subjected to low temperature sweetening (LTS) which result in the production of bitter-tasting, dark colored chips and French fries which are unacceptable to consumers. However, storing tubers at low temperatures (i.e., <10°C) has many advantages such as lowered weight loss during storage, natural control of sprouting, and reduction/elimination of chemical sprout inhibitors. Our earlier research results on LTS suggested a role for pyruvate decarboxylase (PDC) in LTS-tolerance. In the present study, the role of PDC was examined whereby the potato variety Snowden was transformed with Arabidopsis cold-inducible pyruvate decarboxylase gene 1 (AtPDC1) under the control of promoter rd29A. Two transgenic plants were selected and storage studies were conducted on tubers harvested from one of the transgenic lines grown under green house conditions. Transgenic tubers showed higher Agtron chip color score indicating lighter chip and lower reducing sugar and sucrose concentrations compared to the untransformed tubers during the storage periods studied at 12°C and 5°C. These results suggest that overexpression of pyruvate decarboxylase gene resulted in low temperature sweetening tolerance in the transgenic Snowden.

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Section: Discussionsupporting

confidence: 78%

Section: Activity Of Pyruvate Decarboxylasesupporting

confidence: 70%

Section: Introductionmentioning

confidence: 82%

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Alleviation of low temperature sweetening in potato by expressing Arabidopsis pyruvate decarboxylase gene and stress-inducible rd29A: A preliminary study

Pinhero

Pazhekattu

Marangoni

et al. 2011

Physiol Mol Biol Plants

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“…Even rice, which is adapted to life in a flooded environment, incurs damage if the shoots are completely submerged for periods of time (Drew, 1997;Quimio et al, 2000). Genetic variation in flooding tolerance has been found in rice, maize (Zea mays), barley (Hordeum vulgare), and Arabidopsis.…”

mentioning

confidence: 99%

“…A large amount of research has been conducted on the role of ADH, however, to date, no one has overexpressed ADH to determine whether increased ADH activity would improve flooding tolerance. Less research has been done on PDC, but several groups have overexpressed this gene in different plants (Tadege et al, 1998;Quimio et al, 2000), and its importance as a critical control point is becoming apparent. In Arabidopsis, four genes encode PDC activity.…”

mentioning

confidence: 99%

Enhanced Low Oxygen Survival in Arabidopsis through Increased Metabolic Flux in the Fermentative Pathway

Ismond

Dolferus²,

Pauw³

et al. 2003

Plant Physiology

246

212

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We manipulated the enzyme activity levels of the alcohol fermentation pathway, pyruvate decarboxylase (PDC), and alcohol dehydrogenase (ADH) in Arabidopsis using sense and antisense overexpression of the corresponding genes (PDC1, PDC2, and ADH1). Transgenic plants were analyzed for levels of fermentation and evaluated for changes in hypoxic survival. Overexpression of either Arabidopsis PDC1 or PDC2 resulted in improved plant survival. In contrast, overexpression of Arabidopsis ADH1 had no effect on flooding survival. These results support the role of PDC as the control step in ethanol fermentation. Although ADH1 null mutants had decreased hypoxic survival, attempts to reduce the level of PDC activity enough to see an effect on plant survival met with limited success. The combination of flooding survival data and metabolite analysis allows identification of critical metabolic flux points. This information can be used to design transgenic strategies to improve hypoxic tolerance in plants.Plants are constantly challenged by environmental stresses that reduce crop yield (e.g. salinity, low temperature, drought, and flooding). Soils with excess water account for 15.7% of the arable land in the United States, and flooding accounted for 16.4% of the crop insurance claims, making it the second highest cause of crop loss in the United States (Boyer, 1982). Traditionally, plant breeders have selected directly for increased stress tolerance, however, selection for flooding tolerance has been performed only in rice (Oryza sativa), where a genetic mapping approach was used to identify major and minor genes involved in submergence tolerance (Sripongpangkul et al., 2000; Xu et al., 2000). Transgenic plants with increased tolerance to drought, salinity, low temperatures, or a combination of these stresses (Nelson et al., 1998; Huang et al., 2000) were obtained by introducing genes for the overaccumulation of benign compounds (e.g. Gly betaine, mannitol, and Pro) that are believed to act as osmolytes. Other groups have increased stress tolerance in Arabidopsis by overexpressing transcription factors (Jaglo-Ottosen et al., 1998; Kasuga et al., 1999; Yamaguchi-Shinozaki and Shinozaki, 2001) or signal transduction components (Piao et al., 2001; Tamminen et al., 2001).There is substantial variation among terrestrial crop species in their ability to tolerate waterlogging conditions. Even rice, which is adapted to life in a flooded environment, incurs damage if the shoots are completely submerged for periods of time (Drew, 1997; Quimio et al., 2000). Genetic variation in flooding tolerance has been found in rice, maize (Zea mays), barley (Hordeum vulgare), and Arabidopsis. In some cases, this variation results from specific mutants (Harberd and Edwards, 1982; Dolferus et al., 1985; Lemke-Keyes and Sachs, 1989; Ricard et al., 1998). Several studies have focused on the effect of overexpression of genes on flooding tolerance. Zhang et al. (2000) overexpressed a gene involved in cytokinin biosynthesis and demonstrated improve...

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Rice

Emani

Jiang

Hall

et al. 2008

Compendium of Transgenic Crop Plants

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Rice serves as the principal source of nourishment for over half of the global population. The year 2004 was declared as the international year of rice by the Food and Agricultural Organization, to heighten awareness of this crop in combating global poverty and malnutrition. The present review is a comprehensive account of this model monocot crop tracing its history, botanical descriptions, and the research work in breeding and biotechnology. An exhaustive illustrative account of all the important milestones in plant breeding, particle bombardment, Agrobacterium ‐mediated transformation, and genomics has been provided, with a focus on other important aspects such as transgene silencing, biosafety regulations of transgenic plants, and the global consequences of rice biotechnology research.

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Enhancement of Submergence Tolerance in Transgenic Rice Overproducing Pyruvate Decarboxylase

Cited by 91 publications

References 20 publications

Alleviation of low temperature sweetening in potato by expressing Arabidopsis pyruvate decarboxylase gene and stress-inducible rd29A: A preliminary study

Alleviation of low temperature sweetening in potato by expressing Arabidopsis pyruvate decarboxylase gene and stress-inducible rd29A: A preliminary study

Enhanced Low Oxygen Survival in Arabidopsis through Increased Metabolic Flux in the Fermentative Pathway

Rice

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