Enhancement of the activity of papain in mixed reverse micellar systems in the presence of Tween80

Fan, Ke-Ke; Ouyang, Pingkai; Wu, Xijun; Lu, Zhiyong

doi:10.1002/1097-4660(200101)76:1<27::aid-jctb320>3.0.co;2-h

Cited by 18 publications

(5 citation statements)

References 35 publications

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“…In addition, the functional groups within the interior of these assemblies can be used to introduce selectivity. For example, reverse micelles bearing hydrophobic moieties on the outside and hydrophilic groups on the inside provide a scaffold that can readily bind to hydrophilic guest molecules in various biphasic systems. , Small molecule surfactants (e.g., sodium bis(2-ethylhexyl)sulfosuccinate (AOT)) or surfactant/phase transfer reagent mixtures based reverse micelles have been broadly explored for the purposes of protein purification, molecular sensing, biomolecular separations, heterogeneous catalysis, and as nanoreactors. − …”

Section: Introductionmentioning

confidence: 99%

Influence of Charge Density on Host–Guest Interactions within Amphiphilic Polymer Assemblies in Apolar Media

et al. 2017

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The effects of charge density on supramolecular host−guest interactions between peptides and reverse micelles are studied using an amphiphilic random copolymer scaffold. While our classical understanding of polyvalent electrostatic interactions suggests that the binding capacity should increase with charge density, our results indicate that this correlation does not necessarily hold within the nanoscale environment of reverse micelles. Using four series of different polymers, we show that peptide binding efficiency depends on the presence of an optimum charge density inside the reverse micelles. Interestingly, the distribution of charges does not have a significant effect on binding. Reverse micelle stability at high charge densities can be regained by tuning the hydrophilic−lipophilic balance of the polymer, yet an optimal density dependence remains. Our findings improve our understanding of host−guest chemistry in confined aqueous environments.

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Section: Introductionmentioning

confidence: 99%

Influence of Charge Density on Host–Guest Interactions within Amphiphilic Polymer Assemblies in Apolar Media

et al. 2017

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“…It is also possible that these components directly activate the enzyme by interacting with the enzyme and affecting the conformation of the enzyme (Goodman et al, 1967). When the amount of Tween 40 added is a certain value, it will achieve the best emulsifying effect, so that the enzyme and the substrate can be fully contacted (Wyss et al, 2001;Fan et al, 2015). However, the rate of enzymatic hydrolysis does not continue to increase after the amount of Tween added exceeds its optimal amount.…”

Section: Effect Of Tween 40 Addition On Enzymatic Hydrolysis Of Bcaromentioning

confidence: 99%

Purification of β‐carotene 15,15′‐monooxygenase from pig intestine and its enzymatic hydrolysis of pigment in soybean oil

Zhang

Wang

et al. 2018

Int J of Food Sci Tech

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Summary β‐Carotene 15,15′‐monooxygenase was isolated and purified from the intestinal mucosa of pigs, and then, it was applied to hydrolyse the pigment in soybean oil, and thus, vitamin A fortified soybean oil was obtained. The pig intestinal mucosal protein solution was purified to a specific activity of 2.487 × 10−4 U mg−1, maximum reaction velocity (Vmax) of 8.42 × 10−9 mol/h and Michaelis constant (Km) of 2.03 × 10−5 m. The protein solution had a molecular mass of 156 kDa by gel filtration. The sodium deoxycholate concentrations, optimum pH, Tween 40 amount and enzyme amount for vitamin A production in soybean oil were determined to be 6.0 mm, 8.0, 3.0% (w/v) and 0.2 U/mL enzyme, respectively. Under these conditions, β‐carotene 15,15′‐monooxygenase produced 14.65 mg/L vitamin A after 20 h, with a conversion yield of β‐carotene of 33.29% (w/w). Therefore, the nutrients in soybean oil were improved.

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“…With the synthetic substrate, the inhibition was due to a competition between the substrate and the inhibitor molecules for the enzyme (50). Papain is denatured by SLS (51) but its activity can be enhanced with moderate amounts of Tween 80 (52). Analysis of kinetic data showed that SLS acts as a partial non-competitive inhibitor for bromelain, and demonstrated that bromelain is resistant to SLS binding and denaturation (53).…”

Section: Interaction Enzymes-surfactantsmentioning

confidence: 99%

Questions and Answers

Marques¹,

Brown²

2018

Dissolution Technol

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A revision to the general chapters Dissolution <711> and Disintegration and Dissolution of Dietary Supplements <2040> is being proposed to address the shortcomings of the current chapters regarding the use of enzymes in the dissolution test of gelatin capsules or gelatin-coated tablets. The recommendations are to use the enzymes pepsin (medium with pH equal to or below 4.0), papain or bromelain (medium with pH above 4.0 and below 6.8), and pancreatin (medium with pH equal to or above 6.8) if the dosage form does not comply to the appropriate Acceptance Table or, in the case of dietary supplements, appropriate Tolerances requirements due to the presence of cross-linking in the gelatin. Also, the revision clarifies how the enzyme activity should be determined, the amount of enzymes to be added to the medium, and a pre-treatment procedure to be used when the dissolution medium contains surfactant or any other ingredient known or suspect to inactivate the enzyme being used. This Stimuli article provides the rationale for these revisions and presents data to support the recommendations being made. Readers are encouraged to send any comments, questions, suggestions or data to the corresponding author. Also, see the section Other Related Revisions at the end of this article for information on other related general chapters and monographs.

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Enhancement of the activity of papain in mixed reverse micellar systems in the presence of Tween80

Cited by 18 publications

References 35 publications

Influence of Charge Density on Host–Guest Interactions within Amphiphilic Polymer Assemblies in Apolar Media

Influence of Charge Density on Host–Guest Interactions within Amphiphilic Polymer Assemblies in Apolar Media

Purification of β‐carotene 15,15′‐monooxygenase from pig intestine and its enzymatic hydrolysis of pigment in soybean oil

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