Enhancement of the Antihypertensive Effect of Fermented Sargassum horneri with Lactiplantibacillus pentosus SN001

Abstract

Sargassum horneri is a seaweed with antihypertensive properties. However, it is underutilized in some areas, and effective utilization methods are being sought. In this study, we prepared a fermented S. horneri using lactic acid bacteria Lactiplantibacillus pentosus SN001 and investigated its effective utilization by enhancing its antihypertensive effect. The ACE inhibitory activity of S. horneri ranged from 3.6% to a maximum of 63.3% after fermentation. In vivo studies using mice and spontaneously hypertensive rats (SHR) suggested an antihypertensive effect of fermented S. horneri. Purification and NMR analysis of the ACE inhibitory component in fermented S. horneri identified glycerol. Therefore, it is suggested that glycerol is responsible for the strong antihypertensive effect of fermented S. horneri. In conclusion, S. horneri is expected to be used as a dietary ingredient with enhanced antihypertensive effect by fermentation with L. pentosus SN001.

Fermentation of micro- and macroalgae as a way to produce value-added products

“…For example, the biomass of Sargassum horneri (a seaweed with antihypertensive properties) showed an enhancement of this biological activity after lactic acid fermentation by Lactiplantibacillus pentosus bacteria. It was found that glycerol was responsible for the antihypertensive action in this case [98] .…”

Sargassum horneri extract fermented by Lactiplantibacillus pentosus SH803 mediates adipocyte metabolism in 3T3-L1 preadipocytes by regulating oxidative damage and inflammation

“…Among probiotics, strains from the Lactobacillaceae and Bifidobacteriaceae families are involved in fermentation; they regulate microbial growth and the enzymatic conversion of food constituents 6 , 7 , which in turn produces beneficial end-products, such as organic acids, bacteriocins, and peptides 8 . In a previous report, S. horneri fermentation with Lactiplantibacillus pentosus SN001 increased glycerol levels, in which elevated antihypertensive effects 9 . Therefore, fermentation is a prominent tool for enhancing the value of biomaterials.…”

“…S. horneri water extracts and fermented products of those were prepared 44 . S. horneri was washed, dried at 50 °C for 24 h, and ground.…”

“…The resulting powder was mixed with distilled water (1:50 w/v) and autoclaved (121 °C, 15 min) for sterilization. After cooling, cellulase (1:50 v/v, Sigma-Aldrich, MO, USA) was added, incubated (50 °C, pH 4.5) for 48 h, and autoclaved (121 °C, 15 min) for cellulase denaturation and sample sterilization 44 . The resulting mixture was termed S. horneri water extract (SHWE).…”

Inhibition of blood pressure elevation in GABA‐enriched Hidakakombu fermented with Lactiplantibacillus plantarum 002 protected addition of trehalose

“…To activate L. plantarum 002, which had been stored at −80 °C, it was inoculated into ILS (International Lactobacillus Subcommittee) medium according to previous studies (Sekine et al ., 2022; Tamura et al ., 2022) and pre‐cultured (30 °C, 24 h). To 100 mL of 33 mM phosphate buffer solution (pH 5.0), 5 g of Hidakakombu powder was added and stirred.…”