Enhancement of the colorimetric response of enzymatic reactions by thermally evaporated plasmonic thin films: application to glial fibrillary acidic protein

Abel, Biebele; Kabir, Tabassum S.; Odukoya, Babatunde; Mohammed, Muzaffer; Aslan, Kadir

doi:10.1039/c4ay02505a

Cited by 5 publications

(5 citation statements)

References 27 publications

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“…However, the enhancement of chemiluminescence emission on the plasmonic thin films were lower as compared to SIFs with high loading. This observation can be attributed to the topology of the thin films [26], where the extent of enzymes on the thin films are lower than SIFs [27]. This is because proteins are known to adsorb more on roughened surfaces [28].…”

Section: Resultsmentioning

confidence: 89%

Enhancement of the Chemiluminescence Response of Enzymatic Reactions by Plasmonic Surfaces for Biosensing Applications

et al. 2015

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We report the enhancement of chemiluminescence response of horseradish peroxidase (HRP) in bioassays by plasmonic surfaces, which are comprised of (i) silver island films (SIFs) and (ii) metal thin films (silver, gold, copper, and nickel, 1 nm thick) deposited onto glass slides. A model bioassay, based on the interactions of avidin-modified HRP with a monolayer of biotinylated poly(ethylene-glycol)-amine, was employed to evaluate the ability of plasmonic surfaces to enhance chemiluminescence response of HRP. Chemiluminescence response of HRP in model bioassays were increased up to ~3.7-fold as compared to the control samples (i.e. glass slides without plasmonic nanoparticles), where the largest enhancement of the chemiluminescence response was observed on SIFs with high loading. These findings allowed us to demonstrate the use of SIFs (high loading) for the detection of a biologically relevant target protein (glial fibrillary acidic protein or GFAP), where the chemiluminescence response of the standard bioassay for GFAP was enhanced up to ~50% as compared to bioassay on glass slides.

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Section: Resultsmentioning

confidence: 89%

Enhancement of the Chemiluminescence Response of Enzymatic Reactions by Plasmonic Surfaces for Biosensing Applications

et al. 2015

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“…In our previous publications related to the use of silver island films in colorimetric bioassays, we noted that a fraction of the colored product remains on the planar surface and/or that silver island films are removed from the surface during washing steps that result in the removal of enzyme from the surface and loss of colorimetric response [9, 17]. To assess whether the above-mentioned observations still occur on the planar surface studies reported here, the optical absorbance spectra of these surfaces were measured and real-color pictures were visually inspected.…”

Section: Resultsmentioning

confidence: 99%

“…al. demonstrated a ~4-fold increase in the colorimetric response signal on glass surfaces deposited with plasmonic thin films compared to the blank non-modified glass substrates, a clear indication of retained complexes [9]. Subsequently, Abel et.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, one can further modify the nanoparticles with self-assembled monolayers (SAMs) of alkanethiols to covalently link the bioassay components to the surface [11, 16]. However, the use of metal nanoparticles with the planar platforms presents an additional challenge of loss of the nanoparticle films from the surface, and anchor proteins during washing steps of the bioassays, which compromise the efficacy of the bioassays on these platforms [9, 17]. …”

Section: Introductionmentioning

confidence: 99%

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Metal oxide surfaces for enhanced colorimetric response in bioassays

Bonyi

Kukoyi

Daodu

et al. 2017

Colloids and Surfaces B: Biointerfaces

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Physical stability of metal nanoparticle films on planar surfaces can be increased by employing surface modification techniques and/or type of metal nanoparticles. Subsequently, the enzymatic response of colorimetric bioassays can be increased for improved dynamic range for the detection of biomolecules. Using a model bioassay b-BSA, three planar platforms (1) poly (methyl methacrylate) (PMMA) with silver thin films (STFs) (2) silver nanowires (Ag NWs) on paper and (3) indium tin oxide (ITO) on polyethylene terephthalate (PET) were evaluated to investigate the extent of increase in the colorimetric signal. Bioassays for b-BSA and Ki-67 antigen (a real-life bioassay) in buffer were performed using microwave heating (total assay time is 25–30 min) and at room temperature (a control experiment, total assay time is 3 hours). Model bioassays showed that STFs were removed from the surface during washing steps and the extent of ITO remained unchanged. The lowest level of detection (LLOD) for b-BSA bioassays were: 10−10 M for 10 nm STFs on PMMA and Ag NWs on paper and 10−11 M for ITO. Bioassays for Ki-67 antigen yielded a LLOD of <10−9 M on ITO platforms, while STFs platforms were deemed unusable due to significant loss of STFs from the surfaces.

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“…[2][3][4][5][6] Moreover, as reported by several authors, MIFs can exhibit strong plasmonic effects [7][8][9][10] and local electric field enhancement. [11][12][13] Therefore, they can be considered as a building block for nonlinear materials, 14,15 new metamaterial devices, 16 plasmonic sensors, [17][18][19] or transparent screens. 20 Usually, MIFs are characterized by electrical conductivity or transmission spectroscopy measurements.…”

Section: Introductionmentioning

confidence: 99%

Plasmonic and metallic optical properties of Au/SiO2 metal-insulator films

Battie

Naciri

Vergnat

2017

Journal of Applied Physics

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In this paper, the optical properties and the growth mechanism of Au/SiO 2 metal-insulator films (MIFs) are investigated by combining ellipsometry and transmission electron microscopy. The ellipsometric measurements, analyzed by using effective medium theories, show that the growth mechanism involves a Volmer-Weber growth mode while the morphology and the optical properties of Au/SiO 2 MIFs are directly related to the percolation of the Au nanostructures. Indeed, below the percolation threshold of Au, the MIFs consist of ellipsoidal Au inclusions embedded in a SiO 2 matrix. These insulating films present anisotropic plasmonic properties, attributed to the asymmetric interactions between nanaoparticles (NPs), which can be modeled according to the interacted shape distributed nanoparticle effective medium theory. At the percolation threshold of Au, an insulator-to-metal transition is observed. The MIFs simultaneously exhibit plasmonic and metallic optical properties, which can be described by the Bruggeman theory. The density of free electrons increases and the MIFs become more and more conductive as the Au volume fraction increases. We also demonstrate that for a high Au volume fraction, Bruggeman and Maxwell Garnett theories converge toward the same results, suggesting that the film is composed of isolated SiO 2 inclusion embedded in a gold matrix.

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Enhancement of the colorimetric response of enzymatic reactions by thermally evaporated plasmonic thin films: application to glial fibrillary acidic protein

Cited by 5 publications

References 27 publications

Enhancement of the Chemiluminescence Response of Enzymatic Reactions by Plasmonic Surfaces for Biosensing Applications

Enhancement of the Chemiluminescence Response of Enzymatic Reactions by Plasmonic Surfaces for Biosensing Applications

Metal oxide surfaces for enhanced colorimetric response in bioassays

Plasmonic and metallic optical properties of Au/SiO2 metal-insulator films

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