Enhancement of the immune responses to foot-and-mouth disease vaccination in mice by oral administration of a Novel polysaccharide from the roots of Radix Cyathulae officinalis Kuan (RC)

Feng, Haibo; Du, Xiaogang; Tang, Jing; Cao, Xiaohan; Han, Xingfa; Chen, Zhiyu; Chen, Yang-Er; Zeng, Xianyin

doi:10.1016/j.cellimm.2013.02.004

Cited by 35 publications

(31 citation statements)

References 57 publications

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“…However, piglets challenged with HP-PRRSV alone or who received CH-1R did not produce IL-2, which is consistent with other studies of PRRSV infection [19,30]. IL-2 is related to the Th1 responses and used to evaluate candidate vaccines to PRRSV and other viruses [17,31,32]. IL-2 is also necessary during T cell development in the thymus for the maturation of regulatory T cells (T-regs).…”

Section: Discussionsupporting

confidence: 84%

Anti-idiotypic antibodies reduce efficacy of the attenuated vaccine against highly pathogenic PRRSV challenge

Cai

Wang

et al. 2014

BMC Vet Res

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BackgroundThe inability of current vaccines to provide effective protection against porcine reproductive and respiratory syndrome virus (PRRSV) infection is not fully understood. One of the reasons might be the presence of anti-idiotypic antibodies (Ab2s) to the envelope glycoprotein GP5 induced by PRRSV infection since our previous studies demonstrated the presence of auto-Ab2s (aAb2s) in pigs infected with PRRSV. To test this hypothesis, PRRSV negative piglets were injected with a monoclonal Ab2 (Mab2-5G2) and aAb2s that are specific for anti-GP5 antibody, vaccinated with the attenuated PRRSV vaccine CH-1R and then challenged with the highly pathogenic PRRSV HuN4 strain. The animals were evaluated for clinical signs, pathological changes of the thymus and lungs, viremia, levels of serum antibodies and cytokines.ResultsThe piglets injected with Mab2-5G2 or aAb2, and who received the attenuated PRRSV vaccine CH-1R before challenge, produced high levels of anti-N antibodies, IL-2 and IL-4, but low levels of neutralizing antibodies. After PRRSV HuN4 challenge, the animals showed obvious clinical signs, including lung lesions, severe thymus atrophy and decreased production of IL-4 and higher level of viremia.ConclusionWhen anti-GP5 Ab2s are present, the use of attenuated PRRSV vaccine CH-1R against HP-PRRSV infection is not recommended. It can result in poor health status with pneumonia and thymus atrophy.

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Section: Discussionsupporting

confidence: 84%

Anti-idiotypic antibodies reduce efficacy of the attenuated vaccine against highly pathogenic PRRSV challenge

Cai

Wang

et al. 2014

BMC Vet Res

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“…The enhancement of phagocytic function is expected to benefit resistance to microbial infections (Lee & Jeon, 2005). Many studies have reported that Chinese herbal polysaccharides given as an adjuvant could significantly promote the phagocytic function of macrophages (Feng et al, 2013b;Zhang et al, 2010). In the present study, sRCPS increased the PI of peritoneal macrophages in the rHBsAg-immunized mice, especially in the sRCPS4 and sRCPS8 groups, indicating that sRCPS can function as an adjuvant and improve the phagocytic function of macrophages.…”

Section: Discussionsupporting

confidence: 57%

“…The extraction and purification of RCPS was performed as previously described (Feng et al, 2013b). Briefly, the dry root powder was boiled in water three times under reflux.…”

Section: Extraction and Sulfated Modification Of Polysaccharidementioning

confidence: 99%

“…This root is a widely-used Chinese traditional herbal medicine that can improve immune functions in humans and animals (Feng et al, 2013a(Feng et al, , 2013b(Feng et al, , 2014Jia et al, 2009;Ni et al 2011). In our previous studies, Radix Cyathulae officinalis Kuan polysaccharide (RCPS), which was extracted by water decoction and ethanol precipitation, markedly increased both cellular and humoral immune responses (Feng et al, 2013a(Feng et al, , 2013b(Feng et al, , 2014.…”

Section: Introductionmentioning

confidence: 99%

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SulfatedRadix Cyathulae officinalisPolysaccharides Act as Adjuvant via Promoting the Dendritic Cell Maturation and Suppressing Treg Frequency

Feng

Fan

et al. 2015

Immunological Investigations

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This study was conducted to evaluate the adjuvant potential of sulfated Radix Cyathulae officinalis Kuan polysaccharides (sRCPS) and their effects on specific cellular and humoral immune responses to hepatitis B subunit vaccine in mice. Our data demonstrated that sRCPS significantly promoted the rHBsAg-specific IgG, IgG1, IgG2a, and IgG2b antibody titers, the activities of natural killer cells (NK) and cytotoxic T lymphocytes (CTL), T cells proliferation, and phagocytic capacity of peritoneal macrophages. Furthermore, sRCPS increased the levels of IL-4, IL-2, and IFN-γ in CD4(+)T cells and the level of IFN-γ in CD8(+)T cells. In addition, sRCPS enhanced the expression of CD40(+), CD80(+), CD86(+), MHC I and MHC II in dendritic cells (DCs) and upregulated the mRNA levels of MHC I, MHC II. sRCPS downregulated the frequency of CD4(+)CD25(+)Foxp3(+) Treg cells. sRCPS increased both cellular and humoral immune responses by upregulating DC maturation, and suppressing the frequency of Treg cells.

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“…PAMP‐containing adjuvants are typically required to improve the immunogenicity of subunit or vectored vaccines, which lack these ligands. Several studies have documented enhancement of NK cell activation by adjuvants . IL‐15‐matured DCs exposed in vitro to the TLR‐4 agonist AS04‐adjuvanted human papilloma virus (HPV) VLP vaccine can potentiate NK cell activation and killing of HPV‐infected cells compared to either IL‐4‐matured DCs or VLP alone; this effect was attributed to the superior cytokine‐producing ability of the DCs .…”

Section: The Role Of Vaccine Adjuvants In Promoting Nk Cell Responsesmentioning

confidence: 99%

Vaccinating for natural killer cell effector functions

et al. 2018

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Vaccination has proved to be highly effective in reducing global mortality and eliminating infectious diseases. Building on this success will depend on the development of new and improved vaccines, new methods to determine efficacy and optimum dosing and new or refined adjuvant systems. NK cells are innate lymphoid cells that respond rapidly during primary infection but also have adaptive characteristics enabling them to integrate innate and acquired immune responses. NK cells are activated after vaccination against pathogens including influenza, yellow fever and tuberculosis, and their subsequent maturation, proliferation and effector function is dependent on myeloid accessory cell‐derived cytokines such as IL‐12, IL‐18 and type I interferons. Activation of antigen‐presenting cells by live attenuated or whole inactivated vaccines, or by the use of adjuvants, leads to enhanced and sustained NK cell activity, which in turn contributes to T cell recruitment and memory cell formation. This review explores the role of cytokine‐activated NK cells as vaccine‐induced effector cells and in recall responses and their potential contribution to vaccine and adjuvant development.

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Enhancement of the immune responses to foot-and-mouth disease vaccination in mice by oral administration of a Novel polysaccharide from the roots of Radix Cyathulae officinalis Kuan (RC)

Cited by 35 publications

References 57 publications

Anti-idiotypic antibodies reduce efficacy of the attenuated vaccine against highly pathogenic PRRSV challenge

Anti-idiotypic antibodies reduce efficacy of the attenuated vaccine against highly pathogenic PRRSV challenge

SulfatedRadix Cyathulae officinalisPolysaccharides Act as Adjuvant via Promoting the Dendritic Cell Maturation and Suppressing Treg Frequency

Vaccinating for natural killer cell effector functions

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