Enhancement of the separation efficiency through temperature control in preparative high-performance liquid chromatography columns

The reverse auto-compression system: a new type of column for preparative liquid chromatography

“…With large-diameter columns, it is well known that the packing is not homogeneous and less dense in the core of the material. Moreover, the difference in the speed of the flow increases the temperature in the centre of the column as demonstrated by Brandt et al [12,13]. The sum of these two effects produces a tailing of the peak.…”

“…This zone is in part responsible for the peak tailing by slowing down the elution flow and so bending the shape of the chromatographic band [8,9,10,11]. Brandt et al [12,13] have demonstrated that the temperature is not equal in the different parts of the column. This effect has an impact on the viscosity of the mobile phase and influences its velocity in the different parts of the column; thus, the shape of the eluting band is affected and is bent.…”

Estimation of Chromatographic Columns Performances using Computer Tomography and CFD Simulations

“…A lot of results in literature show velocity variations in chromatographic columns by calculations and hence the decrease in separation performance and productivity due to band broadening of the eluting solvent [17,18]. Reasons for the existence of these variations are on one hand the change in mobile phase viscosity and density resulting from temperature fluctuations along the column [19 -21].…”

Study on conical columns with different conical angles for semi-preparative liquid chromatography

“…Many factors influence the flow profile in cylindrical columns [9], such as the viscosity of mobile phase and the solvent used in diluting sample [10,11], the porosity and diameter of frits in the header of columns [12,13], injection modes [7], methods of column packing [14] and temperature gradient [15], etc. In order to avoid interferences from other factors, all the conditions in our experiment were kept identical both in the cylindrical and the conical columns except the column shape.…”