Enhancement of the Upconversion Emission by Visible-to-Near-Infrared Fluorescent Graphene Quantum Dots for miRNA Detection

Laurenti, Marco; Paez-Perez, Miguel; Algarra, Manuel; Alonso-Cristobal, Paulino; López-Cabarcos, Enrique; Méndez-González, Diego; Rubio-Retama, Jorge

doi:10.1021/acsami.6b02361

Cited by 75 publications

(44 citation statements)

References 29 publications

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“…Table compares the different sensing platforms used for miRNA determination. Compared with other methods, the sensitivity of our biosensor is higher than that of some of the methods and approaches that of certain highly sensitive methods . Additionally, our detection system is time saving (analysis complete within 2 h), cost effective, easy to use, and does not involve the synthesis of nanomaterials or the use of expensive and complex chemical modification techniques.…”

Section: Resultsmentioning

confidence: 98%

Nanographite‐based fluorescent biosensor for detecting microRNA using duplex‐specific nuclease‐assisted recycling

Luo

Chen

et al. 2019

Luminescence

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The development of a nanographite (NG)-based fluorescent biosensor for detecting microRNA (miRNA) is reported. Duplex-specific nuclease (DSN)-assisted signal amplification was key to its function. In the absence of a target, with the assistance of pstacking interactions, the NG adsorbed the double carboxyfluorescein (FAM)-labelled probe (DFP) whose surface was perfectly complementary to miRNA, leading to quenching of FAM fluorescence. In the presence of a target, double-stranded DNA/RNA hybrids were repelled by the NG and fluorescence was restored. Meanwhile, the considerable increase in signal strength and sensitivity suggests DSNmediated target recycling as an application. The detection limit of the proposed biosensor for miRNA was 10 pmol/L; there was a linear correlation when the miRNA concentration ranged from 50 pmol/L to 5 nmol/L. Additionally, the method could distinguish let-7b from most let-7 miRNA family members and was successfully used in a sample assay. This biosensor is a novel and highly sensitive tool for miRNA detection and has great potential for biochemical research, disease diagnosis, and therapy. K E Y W O R D Sbiosensor, enzymatic reaction, miRNA, nanographite, target recycling

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Section: Resultsmentioning

confidence: 98%

Nanographite‐based fluorescent biosensor for detecting microRNA using duplex‐specific nuclease‐assisted recycling

Luo

Chen

et al. 2019

Luminescence

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“…In 2016, Laurenti et al reported a biosensor using single stranded DNA (ssDNA) and GQDs, which were coupled with the up-conversion nanoparticles (UCNPs) and silica (SiO 2 ) for determining miRNA sequences [94]. The sensor involved the interaction of sp 2 carbon atoms of the GQDs with ssDNA-UCNPs through π-π stacking, which could bring GQDs to the ssDNA-UCNP/SiO 2 surface.…”

Section: Fluorescence-based Gqd Sensorsmentioning

confidence: 99%

Applications of Graphene Quantum Dots in Biomedical Sensors

Mansuriya

Altıntaş

2020

Sensors

190

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Due to the proliferative cancer rates, cardiovascular diseases, neurodegenerative disorders, autoimmune diseases and a plethora of infections across the globe, it is essential to introduce strategies that can rapidly and specifically detect the ultralow concentrations of relevant biomarkers, pathogens, toxins and pharmaceuticals in biological matrices. Considering these pathophysiologies, various research works have become necessary to fabricate biosensors for their early diagnosis and treatment, using nanomaterials like quantum dots (QDs). These nanomaterials effectively ameliorate the sensor performance with respect to their reproducibility, selectivity as well as sensitivity. In particular, graphene quantum dots (GQDs), which are ideally graphene fragments of nanometer size, constitute discrete features such as acting as attractive fluorophores and excellent electro-catalysts owing to their photo-stability, water-solubility, biocompatibility, non-toxicity and lucrativeness that make them favorable candidates for a wide range of novel biomedical applications. Herein, we reviewed about 300 biomedical studies reported over the last five years which entail the state of art as well as some pioneering ideas with respect to the prominent role of GQDs, especially in the development of optical, electrochemical and photoelectrochemical biosensors. Additionally, we outline the ideal properties of GQDs, their eclectic methods of synthesis, and the general principle behind several biosensing techniques.

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“…In the past few years, UCNPs have demonstrated their potential as excellent molecular beacons (MBs) for analytical biomedical applications. Using UCNP materials, a sensor for detection of specific miRNA sequences based on GQDs and UCNP@SiO 2 ‐ssDNA was developed by Laurenti et al The DNA nucleobases anchored on the UCNPs and the GQDs with sp 2 carbon atoms could interact by means of π‐π stacking, which brought the GQD to the surface of the UCNPs@SiO 2 ‐ssDNA system to enhance the upconversion emission. After hybridization of ssDNA chains and target miRNA, the π‐π stacking between UCNPs and GQDs was destroyed, weakening the fluorescence enhancement.…”

Section: State‐of‐the‐art Protocols For High‐quality Mirna Detectionmentioning

confidence: 99%

Progress in miRNA Detection Using Graphene Material–Based Biosensors

Zhang

Miao

Sun

et al. 2019

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MicroRNAs (miRNAs) are short, endogenous, noncoding RNAs that play critical roles in physiologic and pathologic processes and are vital biomarkers for several disease diagnostics and therapeutics. Therefore, rapid, low‐cost, sensitive, and selective detection of miRNAs is of paramount importance and has aroused increasing attention in the field of medical research. Among the various reported miRNA sensors, devices based on graphene and its derivatives, which form functional supramolecular nanoassemblies of π‐conjugated molecules, have been revealed to have great potential due to their extraordinary electrical, chemical, optical, mechanical, and structural properties. This Review critically and comprehensively summarizes the recent progress in miRNA detection based on graphene and its derivative materials, with an emphasis on i) the underlying working principles of these types of sensors, and the unique roles and advantages of graphene materials; ii) state‐of‐the‐art protocols recently developed for high‐performance miRNA sensing, including representative examples; and iii) perspectives and current challenges for graphene sensors. This Review intends to provide readers with a deep understanding of the design and future of miRNA detection devices.

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Enhancement of the Upconversion Emission by Visible-to-Near-Infrared Fluorescent Graphene Quantum Dots for miRNA Detection

Cited by 75 publications

References 29 publications

Nanographite‐based fluorescent biosensor for detecting microRNA using duplex‐specific nuclease‐assisted recycling

Nanographite‐based fluorescent biosensor for detecting microRNA using duplex‐specific nuclease‐assisted recycling

Applications of Graphene Quantum Dots in Biomedical Sensors

Progress in miRNA Detection Using Graphene Material–Based Biosensors

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