Enhancement of transbilayer mobility of a membrane lipid probe accompanies formation of membrane leaks during photodynamic treatment of erythrocytes

Deüticke, B.; Henseleit, U.; Haest, C.W.M.; Heller, Klaus B.; Dubbelman, T. M. A. R.

doi:10.1016/0005-2736(89)90173-9

Cited by 55 publications

(16 citation statements)

References 45 publications

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“…Then, if membrane damage saturates at the same t irr and higher T irr , a given b, would be attained at a lower T inc . The results of Deuticke et al [8] provide some information about the properties of K + leaks in RBC membranes. Based on the effects of non-electrolytes on K + efflux from RBC photosensitized by aluminum chlorotetrasulfophthalocyanine, they estimated the formation of less than one membrane leak per cell, whose size increased with increasing time of irradiation starting at~0.46 nm.…”

Section: Discussionmentioning

confidence: 96%

A new application of Gompertz function in photohemolysis: the effect of temperature on red blood cell hemolysis photosensitized by protoporphyrin IX

Al-Akhras

2006

Med Bio Eng Comput

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Photosensitization by protoporphyrin IX (PpIX) is accelerated at different irradiation temperatures, different dark incubation temperatures (Tinc) and different irradiation times. The applicability of Gompertz function to the fractional photohemolysis ratio, a and the rate of fractional photohemolysis, b is found to be the most appropriate model to fit the experimental data with minimum parameters and minimum errors. The reduction in Gompertz parameters, the fractional ratio values of a, and increase in the fractional rate values b, for 20 microM PpIX irradiated with black light at low irradiation temperature 5 degrees C and higher Tinc 37 degrees C was noticed. The parameter a has higher values at lower irradiation time and lower irradiation temperatures which indicates a longer photohemolysis process and longer t 50. Values of the parameter b were found to be strongly temperature-dependent, and always increase with increasing irradiation time and Tinc with lower values at lower irradiation time and lower Tinc. There are no significant changes in the lysis of RBCs process at irradiation temperatures equal to or higher than 35 degrees C. Similarly, no significant change on t50 at higher irradiation time at Tinc 24 and 37 degrees C. In conclusion, Gompertz analysis technique adapts to study the photohemolysis process at different conditions as a best-fit model.

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Section: Discussionmentioning

confidence: 96%

A new application of Gompertz function in photohemolysis: the effect of temperature on red blood cell hemolysis photosensitized by protoporphyrin IX

Al-Akhras

2006

Med Bio Eng Comput

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“…2, besides sucrose, the classical impermeant solute to prevent delayed hemolysis, 19 citrate and gluconate also protected thawed cells against hemolysis. The protective effect of mannitol was much less pronounced, indicating that thawed cells have an increased permeability to mannitol and that the radii of the pores formed in the membrane by the freeze‐thaw process are between 0.36 and 0.46, the Stokes radii of mannitol and sucrose, respectively 20 …”

Section: Discussionmentioning

confidence: 99%

Altered processing of thawed red cells to improve the in vitro quality during postthaw storage at 4°C

et al. 2007

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“…4). This indicates that, as compared to untreated cells, phototreated red cells have an increased permeability to mannitol and that the radii of the pores formed in the membrane by DMMB‐phototreatment are between 0·36 and 0·46 nm, the Stokes radii of mannitol and sucrose, respectively [15]. The partial protection by mannitol explains the lower levels of haemolysis found after storage in SAG‐M (40%) as compared to TBS (60%).…”

Section: Discussionmentioning

confidence: 99%

Composition of the additive solution affects red blood cell integrity after photodynamic treatment

et al. 2003

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The additive solution strongly influences the integrity of red cells after photodynamic treatment. Whereas the solution in which the cells are illuminated has a small effect on red cell integrity, the main influence of the additive solution is during post-treatment storage. Red cell integrity is best maintained when illumination is performed in SAG-M followed by storage in AS-3. The presence of non-permeant solutes, such as citrate, in the solution used for storage, prevents haemolysis of the phototreated, cation-permeable cells by counterbalancing the osmotic activity of haemoglobin.

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Enhancement of transbilayer mobility of a membrane lipid probe accompanies formation of membrane leaks during photodynamic treatment of erythrocytes

Cited by 55 publications

References 45 publications

A new application of Gompertz function in photohemolysis: the effect of temperature on red blood cell hemolysis photosensitized by protoporphyrin IX

A new application of Gompertz function in photohemolysis: the effect of temperature on red blood cell hemolysis photosensitized by protoporphyrin IX

Altered processing of thawed red cells to improve the in vitro quality during postthaw storage at 4°C

Composition of the additive solution affects red blood cell integrity after photodynamic treatment

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