Enhancement of transepidermal skin clearing agent delivery using a 980 nm diode laser

Stumpp, Oliver; Welch, Ashley J.; Milner, Thomas E.; Neev, Joseph

doi:10.1002/lsm.20237

Cited by 61 publications

(47 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, Q-switched 1,064-nm Nd:YAG laser irradiation was able to decrease the mean change in reflectance by almost ninefold at 575 nm and eightfold at 615 nm. Stumpp et al [18] reported that by using 980-nm diode laser, they successfully improved the image depth of OCT by about 42%. In their study, some artificial absorption substrates were applied to hamster skin surface to increase light absorption to provide controllable ablation of the SC.…”

Section: Discussionmentioning

confidence: 99%

“…As for the mechanism of light irradiation enhances penetration of drug, it has been reported that lasergenerated stress waves could transiently increase permeability of SC and the cell plasma membrane [3,23,24] or laser irradiation could produce sufficient skin heating to disrupt keratinocyte [3,17,18,25], all of which facilitate glycerol penetration into dermal skin to induce optical skin clearing. Figure 3 shows that the light irradiation is important for control of skin permeation whether it is absorbed by the superficial or deep tissue.…”

Section: Discussionmentioning

confidence: 99%

“…Tuchin et al [17] found that skin permeability could be effectively enhanced by creating an island of limited thermal damage using a flashlamp (IPL) system. Stumpp et al 18 successfully observed that topically treatment of glycerol improved the image depth of optical coherence tomography (OCT) after irradiating the epidermis with a 980 nm diode laser. However, all the previous studies focused on a single light source, both the light irradiation mode and the light dose have not been considered systematically.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Enhancement of skin optical clearing efficacy using photo‐irradiation

Liu¹,

Zhi²,

Tuchin³

et al. 2010

Lasers Surg Med

View full text Add to dashboard Cite

Background and Objectives: Tissue optical clearing technique based on immersion of tissues into optical clearing agents (OCAs) can reduce the scattering and enhance the penetration of light in tissue. However, the barrier function of epidermis limits the penetration of OCAs, and hence is responsible for the poor optical clearing efficacy of skin by topical action. In this study, a variety of light irradiation was applied to increase permeability of agents in skin and improve the optical clearing efficacy. Study Design/Materials and Methods: Different light sources with different dose, i.e, CO 2 laser, Nd:YAG laser (532 and 1,064 nm) with different pulse modes and Intense Pulsed Light (IPL) (400-700 and 560-950 nm) were used to irradiate rat skin in vivo, and then glycerol was applied onto the irradiated zone. VIS-NIR spectrometer was utilized to monitor the changes of reflectance. In vitro skin samples were also irradiated by Q-switched Nd:YAG laser (1,064 nm) and then treated by glycerol for 10-60 minutes. Based on the measurement of the reflectance and transmittance of the samples, the optical properties of skin and penetration depth of light were calculated. Results: Results show that photo-irradiation with appropriate dose combining with the following glycerol treatment is able to reduce in vivo skin reflectance. Compared with the control group, the maximal changes in reflectance are ninefold at 575 nm and eightfold at 615 nm, respectively, which were caused by Q-switched 1,064-nm Nd:YAG laser irradiation and following glycerol treatment. The results for in vitro skin demonstrate that the joint action can significantly increase the optical penetration depth in samples. Conclusions: The combination of Q-switched Nd:YAG (1,064 nm) laser and glycerol could enhance optical skin clearing efficacy significantly. This study provides a noninvasive way to improve the optical clearing of skin, which will benefit the skin optical therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enhancement of skin optical clearing efficacy using photo‐irradiation

Liu¹,

Zhi²,

Tuchin³

et al. 2010

Lasers Surg Med

View full text Add to dashboard Cite

show abstract

“…Beside the chemical agents, a number of physical methods of surmounting the skin barrier are proposed to enhance the diffusion, including low-and high-intensity irradiation [130,158], fractional lamp [131,133] and laser [132] microablation, mechanical microperforation [137,138], ultrasonic (US) irradiation [134,135,159], electrophoresis [160], needleless injection [161], mechanical removal of the surface layer by means of abrasive paper [136], epidermal stripping [162], and microdermabrasion [163].…”

Section: Immersion Clearingmentioning

confidence: 99%

“…The measurements of reflection spectra before and after the exposure have shown that the radiation of Nd:YAG laser in the modes of Qmodulation and long pulses can induce the improvement of transepidermal penetration of OCA by 8-9 folds as compared to the intact skin [130]. In the other study Stumpp et al [158] used a diode laser, operating at the wavelength 980 nm for the rodent skin irradiation using artificially absorbing substrates on the surface. The laser radiation provided heating of the stratum corneum and caused the failure of the protective barrier function.…”

Section: Immersion Clearingmentioning

confidence: 99%

Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Genina

Bashkatov

Sinichkin

et al. 2015

JBPE

View full text Add to dashboard Cite

Abstract.A review of specific features and methods of optical clearing and related interaction of light with tissues is presented. Physical and molecular mechanisms of immersion, compression, and photodynamic/photothermal optical clearing of some fibrous and cellular tissues are discussed. The possibility of efficient control of the tissue optical properties, particularly, the reduction of light scattering in tissues is demonstrated, which facilitates the increased efficiency of various optical visualisation methods (optical biopsy) used in medical purposes. © 2015 Samara State Aerospace University (SSAU).Keywords: tissue, optical clearing, optical diagnostics, imaging. 1, 404-413 (1997). 5. C. L. Smithpeter, A. K. Dunn, A. J. Welch, and R. Richards-Kortum, "Penetration depth limits of in vivo confocal reflectance imaging," Appl. Opt. 37, 2749Opt. 37, -2754Opt. 37, (1998. 6. V. V. Tuchin, L. V. Wang, and D. A. Zimnyakov, Optical Polarization in Biomedical Applications, SpringerVerlag, New York, NY, USA (2006). 7. R. Drezek, A. Dunn, and R. Richards-Kortum, "Light scattering from cells: finite-difference time-domain simulations and goniometric measurements," Appl. Opt. 38(16), 3651-3661 (1999). 8. K. Sokolov, R. Drezek, K. Gossagee, and R. Richards-Kortum, "Reflectance spectroscopy with polarized light:is it sensitive to cellular and nuclear morphology," Opt. Express 5, 302-317 (1999). 9. D. W. Leonard, and K. M. Meek, "Refractive indices of the collagen fibrils and extrafibrillar material of the corneal stroma," Biophysical J. 72, 1382-1387 (1997). 10. A. G. Borovoi, E. I. Naats, and U. G. Oppel, "Scattering of light by a red blood cell," J. Biomed. Opt. 3, 364-372 (1998). 11. A. N. Yaroslavsky, A. V. Priezzhev, J. Rodriguez, I. V. Yaroslavsky, and H. Battarbee, "Optics of blood,"Chap. 2 in Handbook of Optical Biomedical Diagnostics, V. V. Tuchin, Ed., pp. 169-216, PM107 SPIE Press, Bellingham, WA, USA (2002). 12. G. Mazarevica, T. Freivalds, and A. Jurka, "Properties of erythrocyte light refraction in diabetic patients," J.Biomed. Opt. 7, 244-247 (2002). 13. M. Friebel, and M. Meinke, "Model function to calculate the refractive index of native hemoglobin in the wavelength range of 250-1100 nm dependent on concentration," Appl. Opt. 45(12), 2838-2842 (2006). Appl. Opt. 35(19), 3413-3420 (1996). 34. D. Zhu, J. Wang, Z. Zhi, X. Wen, and Q. Luo, "Imaging dermal blood flow through the intact rat skin with an optical clearing method," J. Biomed. Opt. 15, 026008 (2010 241. K. König, G. Flemming, and R. Hibst, "Laser-induced autofluorescence spectroscopy of dental caries lesion," Cell. Mol. Biol. 44, 1293-1300. 242. E. G. Borisova, T. T. Uzunov, and L. A. Avramov, "Early differentiation between caries and tooth demineralization using laser-induced autofluorescence spectroscopy," Lasers Surg. Med. 34, 249-253 (2004 -20(12), 1512-1516 (1984). 245. K. Konig, H. Schneckenburger, and R. Hibst, "Time-gated in vivo autofluorescence imaging of dental caries,"Cell. Mol. Biol. 45, 233-239 (1999). 246. E. Borisova, P. Tr...

show abstract