Enhancement of violaxanthin accumulation in Nannochloropsis oceanica by overexpressing a carotenoid isomerase gene from Phaeodactylum tricornutum

Sun, Yan; Yi, Xin; Zhang, Luyao; Wang, Ying; Liu, Ruolan; Li, Xiaohui; Zhou, Chengxu; Zhang, Lin; Han, Jichang

doi:10.3389/fmicb.2022.942883

Cited by 8 publications

(2 citation statements)

References 92 publications

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“…The most strongly upregulated gene was CRTISO4 (log 2 FC = 2.57), which encodes carotenoid isomerase. Recently, this gene was also proven to participate in the cis-trans isomerization of phytoene, a key gene for lycopene formation (Sun et al, 2022). In addition, most of the enzymes in the fucoxanthin synthesis pathway were upregulated (Figure 5A), which was consistent with increased fucoxanthin content.…”

Section: Transcriptome Data Analysismentioning

confidence: 60%

Overexpression of plastid lipid-associated protein in marine diatom enhances the xanthophyll synthesis and storage

et al. 2023

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Plastoglobules, which are lipoprotein structures surrounded by a single hydrophobic phospholipid membrane, are subcellular organelles in plant chromoplasts and chloroplasts. They contain neutral lipids, tocopherols, quinones, chlorophyll metabolites, carotenoids and their derivatives. Proteomic studies indicated that plastoglobules are involved in carotenoid metabolism and storage. In this study, one of the plastid lipid-associated proteins (PAP), the major protein in plastoglobules, was selected and overexpressed in Phaeodactylum tricornutum. The diameter of the plastoglobules in mutants was decreased by a mean of 19.2% versus the wild-type, while the fucoxanthin level was increased by a mean of 51.2%. All mutants exhibited morphological differences from the wild-type, including a prominent increase in the transverse diameter. Moreover, the unsaturated fatty acid levels were increased in different mutants, including an 18.9–59.3% increase in eicosapentaenoic acid content. Transcriptomic analysis revealed that PAP expression and the morphological changes altered xanthophyll synthesis and storage, which affected the assembly of the fucoxanthin chlorophyll a/c-binding protein and expression of antenna proteins as well as reduced the non-photochemical quenching activity of diatom cells. Therefore, metabolic regulation at the suborganelle level can be achieved by modulating PAP expression. These findings provide a subcellular structural site and target for synthetic biology to modify pigment and lipid metabolism in microalgae chassis cells.

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Section: Transcriptome Data Analysismentioning

confidence: 60%

Overexpression of plastid lipid-associated protein in marine diatom enhances the xanthophyll synthesis and storage

et al. 2023

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“…This resulted in a 1.68‐fold increase in violaxanthin production. [ 181 ] Similarly, overexpression of acetyl‐CoA c‐acetyltransferase in marine microalgae Schizochytrium sp. HX‐308 caused induction of squalene, β‐carotene and astaxanthin biosynthesis.…”

Section: Gene Engineering and Selection Of Microalga For Food And Pig...mentioning

confidence: 99%

Recent advances and fundamentals of microalgae cultivation technology

Rozenberg,

Sorokin,

Mukhambetova

et al. 2024

Biotechnology Journal

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Microalgae are considered to be a promising group of organisms for fuel production, waste processing, pharmaceutical applications, and as a source of food components. Unicellular algae are worth being considered because of their capacity to produce comparatively large amounts of lipids, proteins, and vitamins while requiring little room for growth. They can also grow on waste and fix CO2 and nitrogen compounds. However, production costs limit the industrial use of microalgae to the most profitable applications including micronutrient production and fish farming. Therefore, novel microalgae based technologies require an increase of the production efficiencies or values. Here we review the recent studies focused on getting strains with novel characteristics or cultivating techniques that improve production's robustness or efficiency and categorize these findings according to the fundamental factors that determine microalgae growth. Improvements of light and nutrient delivery, as well as other aspects of photobioreactor design, have shown the highest average increase in productivity. Other methods, such as an improvement of phosphorus or CO2 fixation and temperature adaptation have been found to be less effective. Furthermore, interactions with particular bacteria may promote the growth of microalgae, although bacterial and grazer contaminations must be managed to avoid culture failure. The competitiveness of the algal products will increase if these discoveries are applied to industrial settings.

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An axenic strain reveals the responses of Phaeodactylum tricornutum to external organic carbon

Zhu,

He,

et al. 2024

J. Ocean. Limnol.

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Enhancement of violaxanthin accumulation in Nannochloropsis oceanica by overexpressing a carotenoid isomerase gene from Phaeodactylum tricornutum

Cited by 8 publications

References 92 publications

Overexpression of plastid lipid-associated protein in marine diatom enhances the xanthophyll synthesis and storage

Overexpression of plastid lipid-associated protein in marine diatom enhances the xanthophyll synthesis and storage

Recent advances and fundamentals of microalgae cultivation technology

An axenic strain reveals the responses of Phaeodactylum tricornutum to external organic carbon

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