Enhancement of viral transformation of hamster embryo cells by pretreatment with 4-chloromethylbiphenyl

Hatch, George G.; Mamay, Patricia D.; Nesnow, Stephen

doi:10.1016/0165-1218(82)90051-9

Cited by 5 publications

(2 citation statements)

References 8 publications

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“…Complete adenovirus transformation procedures employed were those previously described [Casto, 1973;Hatch et al, 1982. Following chemical treatment, SHE cells were rinsed and inoculated with SA7 virus ( 3 4 x lo7 plaqueforming units per dish) in 0.2 ml of MEM supplemented with 2% FBS and NaHC03 (0.11 g/100 ml).…”

Section: Transformation Assaysmentioning

confidence: 99%

Analysis of the interlaboratory and intralaboratory reproducibility of the enhancement of simian adenovirus SA7 transformation of syrian hamster embryo cells by model carcinogenic and noncarcinogenic compounds

Schechtman

Hatch²,

Anderson³

et al. 1986

Environ. mutagen

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The intralaboratory and interlaboratory reproducibility of a DNA virus (SA7) transformation enhancement assay was investigated using nine carcinogenic and noncarcinogenic compounds representing a variety of chemical classes. By the use of standardized procedures designed to limit assay variables, replicate assay data were collected in two independent laboratories and analyzed for concurrence. The carcinogens, 7,12-dimethylbenz(a)anthracene, benzo(a)pyrene, and N-methyl-N'-nitro-N-nitrosoguanidine yielded reproducible dose-dependent cytotoxicity and positive transformation effects (defined as statistically significant [p less than or equal to 0.05] enhancement of virus transformation at two or more consecutive dose levels) in all experiments in both laboratories. The carcinogens lead chromate, diethylnitrosamine, 4-nitroquinoline-N-oxide, and 2-acetylaminofluorene demonstrated enhancement of SA7 transformation at two or more dose levels in 40-50% of the assays. The noncarcinogenic structural analogs anthracene and pyrene consistently did not produce positive assay responses when tested at dose levels up to the limits of solubility. Good interlaboratory concurrence was demonstrated for these model compounds in the Syrian hamster embryo cell-SA7 assay.

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Section: Transformation Assaysmentioning

confidence: 99%

Analysis of the interlaboratory and intralaboratory reproducibility of the enhancement of simian adenovirus SA7 transformation of syrian hamster embryo cells by model carcinogenic and noncarcinogenic compounds

Schechtman

Hatch²,

Anderson³

et al. 1986

Environ. mutagen

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“…After EO treatment, SHE cells were rinsed with complete MDM and inoculated with SA7 virus. Adenovirus transformation procedures have been previously reported [Casto, 1968[Casto, , 1973Hatch et al, 1982bHatch et al, , 1983a. SA7 virus was adsorbed for 3 hr (3-4 X lo7 SA7 plaque-forming units per 60-mm Petri dish containing 5-7 x lo6 cells).…”

Section: Quantitation Of Enhanced Sa7 Virus Transformationmentioning

confidence: 99%

Mutation and enhanced virus transformation of cultured hamster cells by exposure to gaseous ethylene oxide

et al. 1986

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Ethylene oxide is a classical mutagen and a carcinogen based on evidence from studies in experimental animals. It is widely distributed in industrial, research, hospital, and food environments. In an effort to explore the use of newly developed methods for exposing mammalian cells to gaseous or volatile mutagens/carcinogens, Chinese hamster V79 cells were treated for 2 hr with gaseous ethylene oxide, in sealed treatment chambers, and assayed for survival and mutagenic response by analysis of induced resistance to 6-thioguanine or ouabain. Significant numbers of mutants were produced at both genetic markers by 1,250-7,500 ppm ethylene oxide. Similarly, primary Syrian hamster embryo cells were treated for 2 or 20 hr with gaseous ethylene oxide in sealed treatment chambers and subsequently assayed for survival and increased sensitivity to SA7 virus transformation. Treatment concentrations extended from toxic to several nontoxic concentrations. After 2-hr ethylene oxide treatment at 625-2,500 ppm a significant enhancement of virus transformation was observed. At 20 hr after treatment no enhancement was observed. Treatment of hamster cells with ethylene oxide in both bioassay systems yielded concentration-related, quantitative results.

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4‐Chlormethylbiphenyl [MAK Value Documentation in German language, 1986]

2012

The MAK‐Collection for Occupational Health and Safety

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Veröffentlicht in der Reihe Gesundheitsschädliche Arbeitsstoffe , 11. Lieferung, Ausgabe 1986 Der Artikel enthält folgende Kapitel: Allgemeiner Wirkungscharakter Erfahrungen beim Menschen Tierexperimentelle Befunde Akute Toxizität Mutagenität Kanzerogenität Zur Frage des MAK‐Wertes

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Enhancement of viral transformation of hamster embryo cells by pretreatment with 4-chloromethylbiphenyl

Cited by 5 publications

References 8 publications

Analysis of the interlaboratory and intralaboratory reproducibility of the enhancement of simian adenovirus SA7 transformation of syrian hamster embryo cells by model carcinogenic and noncarcinogenic compounds

Analysis of the interlaboratory and intralaboratory reproducibility of the enhancement of simian adenovirus SA7 transformation of syrian hamster embryo cells by model carcinogenic and noncarcinogenic compounds

Mutation and enhanced virus transformation of cultured hamster cells by exposure to gaseous ethylene oxide

4‐Chlormethylbiphenyl [MAK Value Documentation in German language, 1986]

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