Enhancement of water-solubilities of protein-bound polysaccharides contained in the basidiocarps ofGanoderma lucidum by hydrolyzing with chymotrypsin

Chromatographic and electrophoretic methods for Lingzhi pharmacologically active components

“…For example, Park et al [121] reported the use of chymotrypsin for the hydrolysis of water-insoluble protein-bound polysaccharides present in G. lucidum, leading to an enhancement of their water solubilities (possibly due to the enzymatic cleavage of the attached hydrophobic polypeptide chains). Also, Peng and Zhang [118] reported the use of trypsin to hydrolyze a water-soluble polysaccharide-protein complex extracted from the mycelium of G. tsugae and determined the protein and carbohydrate contents using size exclusion chromatography.…”

“…Common gel filtration columns that have been employed included Sephadex G-100, G-75, G-50, G-25 [74,86,87,90,91,99,104,132], Sepahrose CL-4B, CL-6B [73,87,89,105,116,117,121,125,127,129,133], Toyopearl HW-65F [85,98], Sephacryl S-200, S-300 [82,86,87,[111][112][113][114] and Fractogel HW-65 [134]. The columns were usually eluted with water or 0.1-0.2 M NaCl.…”