Enhancement of xylanase expression by<i>Komagataella phaffii</i>through pexophagy inhibition

Liu, Taiyu; Zhao, Yixin; Zhang, Jianguo; Zhang, Jining

doi:10.1080/13102818.2019.1623717

Cited by 1 publication

(1 citation statement)

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“…In order to improve xylanase expression under sorbitol-methanol co-feeding conditions, the gene identified in K. phaffii as the initial acceptor of the pexophagy process (ATG30) was deleted to favour peroxisome retention in cells under the influence of the addition of carbon sources that trigger this cellular response. This modification improved xylanase production, reporting an activity of ~1140.7 U/mL in cultures supplemented with 2% sorbitol, representing an increase of ~11.4% compared to the control culture containing 0.5% sorbitol [41]. This demonstrates that by employing cell engineering strategies, the production of a highly in-demand enzyme in the food and paper industry can be successfully increased [53].…”

Section: Engineering Of Co-substrate Catabolic Pathwaysmentioning

confidence: 78%

Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production

et al. 2022

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Komagataella phaffii (formerly known as Pichia pastoris) has become an increasingly important microorganism for recombinant protein production. This yeast species has gained high interest in an industrial setting for the production of a wide range of proteins, including enzymes and biopharmaceuticals. During the last decades, relevant bioprocess progress has been achieved in order to increase recombinant protein productivity and to reduce production costs. More recently, the improvement of cell features and performance has also been considered for this aim, and promising strategies with a direct and substantial impact on protein productivity have been reported. In this review, cell engineering approaches including metabolic engineering and energy supply, transcription factor modulation, and manipulation of routes involved in folding and secretion of recombinant protein are discussed. A lack of studies performed at the higher-scale bioreactor involving optimisation of cultivation parameters is also evidenced, which highlights new research aims to be considered.

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Section: Engineering Of Co-substrate Catabolic Pathwaysmentioning

confidence: 78%