2016
DOI: 10.1016/j.jbiotec.2015.11.002
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Enhancement of yellow pigment production by intraspecific protoplast fusion of Monascus spp. yellow mutant (ade−) and white mutant (prototroph)

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Cited by 24 publications
(14 citation statements)
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“…The strain's MYP production has been further improved to 2224.63 AU 370 /g dry weight (Yongsmith et al 2013). Klinsupa et al (2016) have identified a mutant strain with a high MYP yield (451.0 AU 370 /ml) by fusing intraspecific protoplasts of a yellow mutant with a white prototroph. Beyond that, the mutant strain M. ruber 10910, screened through UV mutagenesis, shows improved production of extracellular hydrophilic MYPs, by [10-fold, with a maximum yield of 134.90 AU 350 /ml (Wu et al 2015a).…”
Section: Strain Improvementmentioning
confidence: 99%
See 1 more Smart Citation
“…The strain's MYP production has been further improved to 2224.63 AU 370 /g dry weight (Yongsmith et al 2013). Klinsupa et al (2016) have identified a mutant strain with a high MYP yield (451.0 AU 370 /ml) by fusing intraspecific protoplasts of a yellow mutant with a white prototroph. Beyond that, the mutant strain M. ruber 10910, screened through UV mutagenesis, shows improved production of extracellular hydrophilic MYPs, by [10-fold, with a maximum yield of 134.90 AU 350 /ml (Wu et al 2015a).…”
Section: Strain Improvementmentioning
confidence: 99%
“…Because of their excellent resistance to photodegradation and their pH and thermal stability (Mapari et al 2009;Su et al 1998;Yang et al 2008), MYPs have been receiving much attention. Except for uses as colorants, MYPs have been reported to possess health benefits, such as in reducing diabetes and obesity (Hsu et al 2014), and have wider applications than MRPs in the food industry (Klinsupa et al 2016). Research continues in the development of improved MYPs yields, as well as in identifying new MYPs (Krairak et al 2000;Chen et al 2015).…”
Section: Introductionmentioning
confidence: 99%
“…Compared with other screening methods such as nutrient deficiency type and drug resistance, parental inactivated protoplast fusion has significantly improved the screening efficiency for fusion recombinants. So far, the inactivated protoplast fusion had been applied in many organisms, such as Solanum tuberosum [14], Candida albicans [15], Bacillus subtilis [16], Saccharomyces cerevisiae [17], and Monascus purpureus [18]. In this way, diploids were formed and the diploids were verified to be stably genetic in some fungi [15,17,18].…”
Section: Introductionmentioning
confidence: 99%
“…So far, the inactivated protoplast fusion had been applied in many organisms, such as Solanum tuberosum [14], Candida albicans [15], Bacillus subtilis [16], Saccharomyces cerevisiae [17], and Monascus purpureus [18]. In this way, diploids were formed and the diploids were verified to be stably genetic in some fungi [15,17,18]. Meanwhile, unfused protoplasts and homokaryotic fusion products will not regenerate.…”
Section: Introductionmentioning
confidence: 99%
“…The pigments produced by Monascus have good coloring property, heat stability, stable oxidizing and reducing effects, acid-base properties, stability when exposed to different metal ions and pH, and other advantages. [14][15][16][17][18][19] The pigments are widely used as a natural coloring agent for fermented food, especially in the food industry of China, Japan, and other southeast Asian countries. 19 However, in the process of fermentation, Monascus can also produce a mycotoxin, citrinin, which is toxic to kidneys, causing teratogenesis, mutagenesis, and carcinogenesis.…”
Section: Introductionmentioning
confidence: 99%