Enhancing a clenbuterol immunosensor based on poly(3,4-ethylenedioxythiophene)/multi-walled carbon nanotube performance using response surface methodology

Abstract

A clenbuterol immunosensor was developed with a poly(3,4-ethylenedioxythiophene)/multi-walled carbon nanotube-modified screen-printed carbon electrode and optimized using response surface methodology.

Development of Highly Sensitive Immunosensor for Clenbuterol Detection by Using Poly(3,4-ethylenedioxythiophene)/Graphene Oxide Modified Screen-Printed Carbon Electrode

“…The unbound linker was removed through the washing process by rinsing with washing buffer. The electrode was further incubated with 10 µL of Ab solution for 1 h at 46 °C [34], followed by rinsing with washing buffer, resulting SPCE/PEDOT/GO/Ab. The optimum immunoassay conditions i.e., antibody concentration, pH, % blocking, immobilization temperature, and immobilization time have been optimized in our previous study [34].…”

“…20 µL of 0.03% dry milk was incubated on the modified surface at 46 °C for 30 min, followed by rinsing with washing buffer. The direct competitive immunoassay was applied for detection of CLB by incubating 10 µL of standard CLB (5 to 150 ng mL −1 ) and the equal amount of CLB-HRP at 46 °C for 33 min [34]. The unbound CLB was removed by rinsing with washing buffer.…”

Nanomaterials for IoT Sensing Platforms and Point-of-Care Applications in South Korea

“…By the layer-by-layer coating, 1D MWCNTs were intercalated to 2D MXene-Ti 3 C 2 T x nanosheets to provide a "bridging effect" as shown in the SEM image (Figure 12d) [154]. As a result, the hybrid structure of MWCNT/MXene-Ti 3 C 2 T x exhibited excellent electrical conductivity and a large surface area.…”

A label-free aptasensor for rapid detection of clenbuterol based on SYBR Green I

“…In order to ensure the safety of meat-based food products, it is necessary to monitor the residues of clenbuterol regularly. Various methods, including immunoassays, 7,8 chromatographybased techniques, [9][10][11] fluorescence assays, 12,13 electrochemical methods 14,15 and combinatorial techniques, 16,17 have been established for the detection of CLB. Immunoassays and chromatography-based methods are the main analytical methods.…”