Enhancing alfalfa resistance to Spodoptera herbivory by sequestering microRNA396 expression

Yan, Jianping; Qiu, Rumeng; Wang, Kexin; Liu, Yanrong; Zhang, Wanjun

doi:10.1007/s00299-023-02993-z

Cited by 8 publications

(4 citation statements)

References 70 publications

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“…The first microRNA (miRNA) identified to involve the immune response was miR393, which was induced by flg22 to repress auxin signaling by silencing its receptors [22]. In addition to miR393, other miRNAs, such as miR159, miR160, miR166, miR167, miR396, miR398, and miR408, also play essential roles in plant defense [21,[23][24][25][26][27].…”

Section: Discussionmentioning

confidence: 99%

“…Currently, the phenomenon of RNA interference (RNAi) and sRNAs are also considered important regulators of gene expression reprogramming in plant immune responses, pathogen or pest virulence, and communication in plant-microbial interactions [21][22][23][24][25][26][27]. Previous studies showed that miRNAs that induce RNAi are involved in plant response to sap-sucking insects.…”

Section: Introductionmentioning

confidence: 99%

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Bacillus subtilis 26D Triggers Induced Systemic Resistance against Rhopalosiphum padi L. by Regulating the Expression of Genes AGO, DCL and microRNA in Bread Spring Wheat

Rumyantsev,

Veselova,

Burkhanova

et al. 2023

Microorganisms

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Bacillus subtilis 26D is a plant growth-promoting endophytic bacteria capable of inducing systemic resistance through the priming mechanism, which includes plant genome reprogramming and the phenomenon of RNA interference (RNAi) and microRNA (miRNAs). The phloem-feeding insect bird cherry-oat aphid Rhopalosiphum padi L. is a serious pest that causes significant damage to crops throughout the world. However, the function of plant miRNAs in the response to aphid infestation remains unclear. The results of this work showed that B. subtilis 26D stimulated aphid resistance in wheat plants, inducing the expression of genes of hormonal signaling pathways ICS, WRKY13, PR1, ACS, EIN3, PR3, and ABI5. In addition, B. subtilis 26D activated the RNAi mechanism and regulated the expression of nine conserved miRNAs through activation of the ethylene, salicylic acid (SA), and abscisic acid (ABA) signaling pathways, which was demonstrated by using treatments with phytohormones. Treatment of plants with SA, ethylene, and ABA acted in a similar manner to B. subtilis 26D on induction of the expression of the AGO4, AGO5 and DCL2, DCL4 genes, as well as the expression of nine conserved miRNAs. Different patterns of miRNA expression were found in aphid-infested plants and in plants treated with B. subtilis 26D or SA, ethylene, and ABA and infested by aphids, suggesting that miRNAs play multiple roles in the plant response to phloem-feeding insects, associated with effects on hormonal signaling pathways, redox metabolism, and the synthesis of secondary metabolites. Our study provides new data to further elucidate the fine mechanisms of bacterial-induced priming. However, further extensive work is needed to fully unravel these mechanisms.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bacillus subtilis 26D Triggers Induced Systemic Resistance against Rhopalosiphum padi L. by Regulating the Expression of Genes AGO, DCL and microRNA in Bread Spring Wheat

Rumyantsev,

Veselova,

Burkhanova

et al. 2023

Microorganisms

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“…According to our earlier studies, those calluses lines were used as explants to produce MIM396 transgenic plants, respectively. The recombinant vector of overexpression MIM396 gene was reported in our previously reported method [ 48 ]. These transgenic plants were grown in a greenhouse with 16 h light/8 h dark at 25 °C.…”

Section: Methodsmentioning

confidence: 99%

Blocking miR396 activity by overexpression MIM396 improved switchgrass tiller number and biomass yield

Xu,

Li,

Yan

et al. 2024

Biotechnol Biofuels

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Background MicroRNA396 (miR396) plays an important role in the regulation of plant growth and development by repressing the expression level of its target growth-regulating factor (GRF) family genes. In our previous study, we found that overexpression of miR396 negatively regulated both tillering and biomass yield in switchgrass (Panicum virgatum L.). We, therefore, speculated that blocking the expression of miR396 could enhance switchgrass tillering and biomass yield. Here, we produced transgenic switchgrass plants overexpressing a target mimicry form of miR396 (MIM396) in wild type (WT) and Os-MIR319b overexpressing switchgrass plant (with higher enzymatic hydrolysis efficiency, but reduced tillering), in which the expression of miR396 was blocked. The phenotype and biological yields of these plants were analyzed. Results Blocking miR396 to improve its target PvGRFs expression in switchgrass improved the tiller number and dry weight of transgenic plants. Further morphological analysis revealed that MIM396 plants increased the number of aerial branches and basal tillers compared to those of wild-type plants. The enzymatic efficiency of MIM396 plants was reduced; however, the total sugar production per plant was still significantly higher than that of wild-type plants due to the increase in biomass. In addition, blocking miR396 in a transgenic switchgrass plant overexpressing Os-MIR319b (TG21-Ms) significantly increased the PvGRF1/3/5 expression level and tiller number and biomass yield. The miR156-target gene PvSPL4, playing a negative role in aerial and basal buds outgrowth, showed significant downregulated in MIM396 and TG21-Ms. Those results indicate that miR396-PvGRFs, through disrupting the PvSPL4 expression, are involved in miR319-PvPCFs in regulating tiller number, at least partly. Conclusions MIM396 could be used as a molecular tool to improving tiller number and biomass yield in switchgrass wild type and miR319b transgenic plants. This finding may be applied to other graminaceous plants to regulate plant biological yield.

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“…Furthermore, miR396 and GRF8-OE plants exhibit increased flavonoid content, which is associated with enhanced pathogen resistance, revealing a novel pathogen resistance mechanism mediated by the miR396-GRF8-F3H-flavonoid pathway [102]. In contrast, overexpression of miR396 in alfalfa enhances resistance to Spodoptera litura larvae; this may be attributed to the increased lignin content and enhanced biosynthesis of lowmolecular-weight flavonoids and glucosinolates [103].…”

Section: Mirnas Regulate Reactive Oxygen Species Accumulation and Pla...mentioning

confidence: 99%

microRNAs: Key Regulators in Plant Responses to Abiotic and Biotic Stresses via Endogenous and Cross-Kingdom Mechanisms

Ding,

Li,

et al. 2024

IJMS

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Dramatic shifts in global climate have intensified abiotic and biotic stress faced by plants. Plant microRNAs (miRNAs)—20–24 nucleotide non-coding RNA molecules—form a key regulatory system of plant gene expression; playing crucial roles in plant growth; development; and defense against abiotic and biotic stress. Moreover, they participate in cross-kingdom communication. This communication encompasses interactions with other plants, microorganisms, and insect species, collectively exerting a profound influence on the agronomic traits of crops. This article comprehensively reviews the biosynthesis of plant miRNAs and explores their impact on plant growth, development, and stress resistance through endogenous, non-transboundary mechanisms. Furthermore, this review delves into the cross-kingdom regulatory effects of plant miRNAs on plants, microorganisms, and pests. It proceeds to specifically discuss the design and modification strategies for artificial miRNAs (amiRNAs), as well as the protection and transport of miRNAs by exosome-like nanovesicles (ELNVs), expanding the potential applications of plant miRNAs in crop breeding. Finally, the current limitations associated with harnessing plant miRNAs are addressed, and the utilization of synthetic biology is proposed to facilitate the heterologous expression and large-scale production of miRNAs. This novel approach suggests a plant-based solution to address future biosafety concerns in agriculture.

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Enhancing alfalfa resistance to Spodoptera herbivory by sequestering microRNA396 expression

Cited by 8 publications

References 70 publications

Bacillus subtilis 26D Triggers Induced Systemic Resistance against Rhopalosiphum padi L. by Regulating the Expression of Genes AGO, DCL and microRNA in Bread Spring Wheat

Bacillus subtilis 26D Triggers Induced Systemic Resistance against Rhopalosiphum padi L. by Regulating the Expression of Genes AGO, DCL and microRNA in Bread Spring Wheat

Blocking miR396 activity by overexpression MIM396 improved switchgrass tiller number and biomass yield

microRNAs: Key Regulators in Plant Responses to Abiotic and Biotic Stresses via Endogenous and Cross-Kingdom Mechanisms

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