Enhancing anaerobic digestion of lignocellulosic materials in excess sludge by bioaugmentation and pre-treatment

Hu, Yuansheng; Hao, Xiaodi; Wang, Jimin; Cao, Yingjie

doi:10.1016/j.wasman.2015.12.006

Cited by 77 publications

(35 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Biogas intensification by bioaugmentation with cellulolytic bacteria for improving hydrolysis has been reported in several biogas studies from untreated lignocellulosic substrates [ 15 , 16 ] and only few studies from pretreated materials [ 41 , 42 ]. In a batch experiment fed with untreated straw, bioaugmentation increased the methane yield by 27% in a 4% amended enrichment culture obtained from sheep rumen fluid containing cellulolytic bacteria [ 15 ].…”

Section: Resultsmentioning

confidence: 99%

“…For instance, most of the bioaugmentation studies described above used untreated lignocellulosic substrates and obtained only a maximum of 40% improvement in methane yield as a result of bioaugmentation. However, combined pretreatment and bioaugmentation to enhance methane production has achieved 210–246% increase in methane yields (Hu et al [ 42 ]). Therefore, pretreatment followed by bioaugmentation seems like a very promising strategy to produce methane from lignocellulosic biomass.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enhancing methane production from lignocellulosic biomass by combined steam-explosion pretreatment and bioaugmentation with cellulolytic bacterium Caldicellulosiruptor bescii

Mulat

Huerta

Kalyani

et al. 2018

Biotechnol Biofuels

View full text Add to dashboard Cite

BackgroundBiogas production from lignocellulosic biomass is generally considered to be challenging due to the recalcitrant nature of this biomass. In this study, the recalcitrance of birch was reduced by applying steam-explosion (SE) pretreatment (210 °C and 10 min). Moreover, bioaugmentation with the cellulolytic bacterium Caldicellulosiruptor bescii was applied to possibly enhance the methane production from steam-exploded birch in an anaerobic digestion (AD) process under thermophilic conditions (62 °C).ResultsOverall, the combined SE and bioaugmentation enhanced the methane yield up to 140% compared to untreated birch, while SE alone contributed to the major share of methane enhancement by 118%. The best methane improvement of 140% on day 50 was observed in bottles fed with pretreated birch and bioaugmentation with lower dosages of C. bescii (2 and 5% of inoculum volume). The maximum methane production rate also increased from 4-mL CH4/g VS (volatile solids)/day for untreated birch to 9–14-mL CH4/g VS/day for steam-exploded birch with applied bioaugmentation. Bioaugmentation was particularly effective for increasing the initial methane production rate of the pretreated birch yielding 21–44% more methane than the pretreated birch without applied bioaugmentation. The extent of solubilization of the organic matter was increased by more than twofold when combined SE pretreatment and bioaugmentation was used in comparison with the methane production from untreated birch. The beneficial effects of SE and bioaugmentation on methane yield indicated that biomass recalcitrance and hydrolysis step are the limiting factors for efficient AD of lignocellulosic biomass. Microbial community analysis by 16S rRNA amplicon sequencing showed that the microbial community composition was altered by the pretreatment and bioaugmentation processes. Notably, the enhanced methane production by pretreatment and bioaugmentation was well correlated with the increase in abundance of key bacterial and archaeal communities, particularly the hydrolytic bacterium Caldicoprobacter, several members of syntrophic acetate oxidizing bacteria and the hydrogenotrophic Methanothermobacter.ConclusionOur findings demonstrate the potential of combined SE and bioaugmentation for enhancing methane production from lignocellulosic biomass.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhancing methane production from lignocellulosic biomass by combined steam-explosion pretreatment and bioaugmentation with cellulolytic bacterium Caldicellulosiruptor bescii

Mulat

Huerta

Kalyani

et al. 2018

Biotechnol Biofuels

View full text Add to dashboard Cite

show abstract

“…As reported earlier [ 22 , 43 , 44 ], B. cellulosolvens grows on cellulose and cellobiose as sole carbon sources but is also able to degrade xylan and has high xylanase activity in secreted and cell-associated fractions (Additional file 2 : Figure S1). Recently, it was shown that B. cellulosolvens is a highly active lignocellulolytic microorganism able to efficiently digest cellulose, hemicellulose, and lignin together with Clostridium stercorarium [ 45 ]. Here, CAZy analysis revealed 147 GH modules, with a wide array of cellulolytic and hemicellulolytic enzymes, either cellulosomal (about 60% containing dockerins) or free enzymes (40%).…”

Section: Resultsmentioning

confidence: 99%

Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system

Zhivin

Dassa

Moraïs

et al. 2017

Biotechnol Biofuels

View full text Add to dashboard Cite

Background (Pseudo) Bacteroides cellulosolvens is an anaerobic, mesophilic, cellulolytic, cellulosome-producing clostridial bacterium capable of utilizing cellulose and cellobiose as carbon sources. Recently, we sequenced the B. cellulosolvens genome, and subsequent comprehensive bioinformatic analysis, herein reported, revealed an unprecedented number of cellulosome-related components, including 78 cohesin modules scattered among 31 scaffoldins and more than 200 dockerin-bearing ORFs. In terms of numbers, the B. cellulosolvens cellulosome system represents the most intricate, compositionally diverse cellulosome system yet known in nature.ResultsThe organization of the B. cellulosolvens cellulosome is unique compared to previously described cellulosome systems. In contrast to all other known cellulosomes, the cohesin types are reversed for all scaffoldins i.e., the type II cohesins are located on the enzyme-integrating primary scaffoldin, whereas the type I cohesins are located on the anchoring scaffoldins. Many of the type II dockerin-bearing ORFs include X60 modules, which are known to stabilize type II cohesin–dockerin interactions. In the present work, we focused on revealing the architectural arrangement of cellulosome structure in this bacterium by examining numerous interactions between the various cohesin and dockerin modules. In total, we cloned and expressed 43 representative cohesins and 27 dockerins. The results revealed various possible architectures of cell-anchored and cell-free cellulosomes, which serve to assemble distinctive cellulosome types via three distinct cohesin–dockerin specificities: type I, type II, and a novel-type designated R (distinct from type III interactions, predominant in ruminococcal cellulosomes).ConclusionsThe results of this study provide novel insight into the architecture and function of the most intricate and extensive cellulosomal system known today, thereby extending significantly our overall knowledge base of cellulosome systems and their components. The robust cellulosome system of B. cellulosolvens, with its unique binding specificities and reversal of cohesin–dockerin types, has served to amend our view of the cellulosome paradigm. Revealing new cellulosomal interactions and arrangements is critical for designing high-efficiency artificial cellulosomes for conversion of plant-derived cellulosic biomass towards improved production of biofuels.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-017-0898-6) contains supplementary material, which is available to authorized users.

show abstract

“…Lignins [1][2][3][4][5][6][7][8][9][10], in particular, and in general lignocellulosic materials (LCMs) [11][12][13][14][15][16] and biomass [17][18][19][20], respectively, have historically been utilised since time memorial, but a new conversation is emerging on the role of these materials in modern bioeconomies [21][22][23][24][25]. Due to the nature of the molecule(s) as a novel commodity for many interesting natural and manufactured products [26][27][28][29], a modern bioeconomy [30][31][32][33] is not simply a rerun of former ones.…”

Section: Introductionmentioning

confidence: 99%

Utilisation of Lignins in the Bioeconomy: Projections on Ionic Liquids and Molecularly Imprinted Polymers for Selective Separation and Recovery of Base Metals and Gold

Ndibewu¹,

Tchieta²

2018

Lignin - Trends and Applications

View full text Add to dashboard Cite

A brief review has been herein done of technologies involved in the exploitation of lignin, in order to provide an introduction to the subject from the perspective of a fast technologically advancing economy. Lignocellulosic materials and biomass have historically been utilised from since time memorial, but a new conversation is emerging on the role of these materials in modern bioeconomies. This new discourse needs to help us understand how technologies for managing and processing lignocellulosic materials both as biosynthetic moieties, biogenic wastes or simply renewable biopolymer-both established and novel-should be deployed and integrated (or not) to meet developmental requirements of the sustainability paradigm. The world is caught in the middle of green technology advocating for more and more focus on renewable sources of manufacturing raw materials and that of the molecularly imprinted/synthesised or genetically engineered ones. The utilisation of lignins (from renewable sources) in both the industry as the base for the formulation of ionic liquids (with yet a wider industrial applications), and also is a potential scaffold material for functionally modified imprinted polymers (LCIPS) for the selective recovery of base metals and gold, respectively, evidently incorporates the bioeconomy aspirations.

show abstract

Enhancing anaerobic digestion of lignocellulosic materials in excess sludge by bioaugmentation and pre-treatment

Cited by 77 publications

References 21 publications

Enhancing methane production from lignocellulosic biomass by combined steam-explosion pretreatment and bioaugmentation with cellulolytic bacterium Caldicellulosiruptor bescii

Enhancing methane production from lignocellulosic biomass by combined steam-explosion pretreatment and bioaugmentation with cellulolytic bacterium Caldicellulosiruptor bescii

Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system

Utilisation of Lignins in the Bioeconomy: Projections on Ionic Liquids and Molecularly Imprinted Polymers for Selective Separation and Recovery of Base Metals and Gold

Contact Info

Product

Resources

About