Enhancing biocompatibility and neuronal anti-inflammatory activity of polymyxin B through conjugation with gellan gum

Peng, Xingqiao; Zhu, Lan; Guo, Jiayun; Sun, Zhenglong; Zhao, Mingming; Zhan, Xi

doi:10.1016/j.ijbiomac.2019.12.200

Cited by 14 publications

(3 citation statements)

References 31 publications

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“…After cooling to room temperature, the supernatant were centrifuged at 10,000 × g for 20 min to be measured as described in the manufacturer’s manual (Comin, Suzhou, China). Fungal cultures on SYA at 28°C for hours were collected and washed three times with sterile water for NO content detection using a visible spectrophotometric NO content detection kit (Solarbio, Beijing, China) as described previously ( 60 ).…”

Section: Methodsmentioning

confidence: 99%

MaNCP1, a C2H2 Zinc Finger Protein, Governs the Conidiation Pattern Shift through Regulating the Reductive Pathway for Nitric Oxide Synthesis in the Filamentous Fungus Metarhizium acridum

et al. 2022

Microbiol Spectr

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Section: Methodsmentioning

confidence: 99%

MaNCP1, a C2H2 Zinc Finger Protein, Governs the Conidiation Pattern Shift through Regulating the Reductive Pathway for Nitric Oxide Synthesis in the Filamentous Fungus Metarhizium acridum

et al. 2022

Microbiol Spectr

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“…Hence, it is necessary to develop an appropriate drug delivery system to improve the selectivity of MDPs. To improve the selectivity of PMB and reduce its nephro- and neurotoxicity, several polymyxin formulations have been reported, such as SLNs 202 , polyion complexes 203 , liposomes 204 , and polysaccharide materials 205 . Recently, Arpanaei's group 206 reported that anionic, functionally mesoporous silica nanoparticles could act as potential carriers for PMB delivery, because they could encapsulate a high load of PMB and retain antibacterial activity with enhanced biocompatibility.…”

Section: Mdps-based Formulations and Their Applicationsmentioning

confidence: 99%

Membrane-disruptive peptides/peptidomimetics-based therapeutics: Promising systems to combat bacteria and cancer in the drug-resistant era

Lin

Chi

Yan

et al. 2021

Acta Pharmaceutica Sinica B

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Membrane-disruptive peptides/peptidomimetics (MDPs) are antimicrobials or anticarcinogens that present a general killing mechanism through the physical disruption of cell membranes, in contrast to conventional chemotherapeutic drugs, which act on precise targets such as DNA or specific enzymes. Owing to their rapid action, broad-spectrum activity, and mechanisms of action that potentially hinder the development of resistance, MDPs have been increasingly considered as future therapeutics in the drug-resistant era. Recently, growing experimental evidence has demonstrated that MDPs can also be utilized as adjuvants to enhance the therapeutic effects of other agents. In this review, we evaluate the literature around the broad-spectrum antimicrobial properties and anticancer activity of MDPs, and summarize the current development and mechanisms of MDPs alone or in combination with other agents. Notably, this review highlights recent advances in the design of various MDP-based drug delivery systems that can improve the therapeutic effect of MDPs, minimize side effects, and promote the co-delivery of multiple chemotherapeutics, for more efficient antimicrobial and anticancer therapy.

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“…It was hypothesized that functionalization with vitamin B12 would enhance intestinal permeability and absorption of the polymeric conjugates through receptor-mediated endocytosis. The concept of conjugating CT to polymers to improve its biopharmaceutical properties has been used previously [25][26][27][28]. However, to the best of our knowledge, this study was the first attempt to develop an oral form of CT that can be absorbed in the GI tract by grafting vitamin B12 as a targeting ligand.…”

Section: Introductionmentioning

confidence: 99%

Cyanocobalamin-Modified Colistin–Hyaluronan Conjugates: Synthesis and Bioactivity

Dubashynskaya

Bokatyi

Sall

et al. 2023

IJMS

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Polymeric drug delivery systems enhance the biopharmaceutical properties of antibiotics by increasing their bioavailability, providing programmable and controlled-release properties, and reducing toxicity. In addition, drug delivery systems are a promising strategy to improve the intestinal permeability of various antimicrobial agents, including colistin (CT). This study describes the modification of conjugates based on CT and hyaluronic acid (HA) with cyanocobalamin (vitamin B12). Vitamin B12 was chosen as a targeting ligand because it has its own absorption pathway in the small intestine. The resulting polysaccharide conjugates contained 95 μg/mg vitamin B12 and the CT content was 335 μg/mg; they consisted of particles of two sizes, 98 and 702 nm, with a ζ-potential of approximately −25 mV. An in vitro release test at pH 7.4 and pH 5.2 showed an ultra-slow release of colistin of approximately 1% after 10 h. The modified B12 conjugates retained their antimicrobial activity at the level of pure CT (minimum inhibitory concentration was 2 μg/mL). The resulting delivery systems also reduced the nephrotoxicity of CT by 30–40% (HEK 293 cell line). In addition, the modification of B12 improved the intestinal permeability of CT, and the apparent permeability coefficient of HA–CT–B12 conjugates was 3.5 × 10−6 cm/s, corresponding to an in vivo intestinal absorption of 50–100%. Thus, vitamin-B12-modified conjugates based on CT and HA may be promising oral delivery systems with improved biopharmaceutical properties.

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Enhancing biocompatibility and neuronal anti-inflammatory activity of polymyxin B through conjugation with gellan gum

Cited by 14 publications

References 31 publications

MaNCP1, a C2H2 Zinc Finger Protein, Governs the Conidiation Pattern Shift through Regulating the Reductive Pathway for Nitric Oxide Synthesis in the Filamentous Fungus Metarhizium acridum

MaNCP1, a C2H2 Zinc Finger Protein, Governs the Conidiation Pattern Shift through Regulating the Reductive Pathway for Nitric Oxide Synthesis in the Filamentous Fungus Metarhizium acridum

Membrane-disruptive peptides/peptidomimetics-based therapeutics: Promising systems to combat bacteria and cancer in the drug-resistant era

Cyanocobalamin-Modified Colistin–Hyaluronan Conjugates: Synthesis and Bioactivity

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