Enhancing cell seeding of scaffolds in tissue engineering through manipulation of hydrodynamic parameters

Bueno, Ericka M.; Laevsky, Gary; Barabino, Gilda A.

doi:10.1016/j.jbiotec.2007.01.005

Cited by 60 publications

(54 citation statements)

References 41 publications

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“…The first step targets single hESC adhesion onto the MC surface within 1-2 h, with a high degree of efficiency. This initial cell seeding, which is critical to the success of this process, needs to occur in the presence of hydrodynamic forces that are generated by agitation [30]. Delayed cell attachment generally leads to cell death and can induce the development of differentiating EB [25].…”

Section: Resultsmentioning

confidence: 99%

“…Binding interactions between endogenous integrins, found in the hESC membrane, and exogenous ECM protein, presented at the MC surface, lead to a cascade of signaling events. This results in cytoskeleton assembly and the subsequent initiation of cell division [30]. With shear gradients and putative MC collisions known to affect cell spreading [35,36,40,50], MC surface properties that generate sufficiently robust cell attachment and engender their efficient spreading in agitated cultures represent a critical enabling technology for efficient cell growth under agitation.…”

Section: Resultsmentioning

confidence: 99%

“…VN exhibits a significantly different structure. This multifunctional monomeric glycoprotein, which is found in both plasma and the ECM [29], adsorbs to surfaces [30]. PS substrates coated with VN promote hESC attachment [20,31] and support their longterm expansion [31][32][33][34], exhibiting performance on par with LN and Matrigel [18,[31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cationic Surface Charge Combined with Either Vitronectin or Laminin Dictates the Evolution of Human Embryonic Stem Cells/Microcarrier Aggregates and Cell Growth in Agitated Cultures

Lam

Li²,

Chen³

et al. 2014

Stem Cells and Development

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cationic Surface Charge Combined with Either Vitronectin or Laminin Dictates the Evolution of Human Embryonic Stem Cells/Microcarrier Aggregates and Cell Growth in Agitated Cultures

Lam

Li²,

Chen³

et al. 2014

Stem Cells and Development

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“…42 The synthetic scaffold should act to promote the optimization of growth and cell development in injured tissue. It must be implanted, seeded with cells, directly into the affected area of tissue to be regenerated, 43 or seeded with cells and cultured in vitro with the intention of forming the new tissue prior to implantation. 44 Then the entire process of reconstitution occurs, from the successful interaction between cells and cells and ECM, avoiding initiation of an immune response.…”

Section: Required Characteristics For a Desirable Scaffold And Its Immentioning

confidence: 99%

Carbon nanotube interaction with extracellular matrix proteins producing scaffolds for tissue engineering

Tonelli¹,

Santos²,

Gomes³

et al. 2012

IJN

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Abstract:In recent years, significant progress has been made in organ transplantation, surgical reconstruction, and the use of artificial prostheses to treat the loss or failure of an organ or bone tissue. In recent years, considerable attention has been given to carbon nanotubes and collagen composite materials and their applications in the field of tissue engineering due to their minimal foreign-body reactions, an intrinsic antibacterial nature, biocompatibility, biodegradability, and the ability to be molded into various geometries and forms such as porous structures, suitable for cell ingrowth, proliferation, and differentiation. Recently, grafted collagen and some other natural and synthetic polymers with carbon nanotubes have been incorporated to increase the mechanical strength of these composites. Carbon nanotube composites are thus emerging as potential materials for artificial bone and bone regeneration in tissue engineering.

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“…The unique hydrodynamic environment within the WWB was characterized using computational fluid dynamics simulation, which was further validated by particle image velocimetry methods. 10,11 Compared with the spinner flask, the WWB yields enhanced chondrocyte aggregation, 12 increased cellseeding efficiency, 13 and improved cell proliferation and extracellular matrix (ECM) deposition within constructs. 14,15 An artificial neural network model was established to correlate hydrodynamic parameters with functional properties of neocartilage grown within the WWB and suggests an optimal agitation rate of 50 rpm.…”

Section: Introductionmentioning

confidence: 99%

Differential Morphology and Homogeneity of Tissue-Engineered Cartilage in Hydrodynamic Cultivation with Transient Exposure to Insulin-Like Growth Factor-1 and Transforming Growth Factor-β1

Yang

Barabino

2013

Tissue Engineering Part A

Self Cite

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Successful tissue-engineering strategies for cartilage repair must maximize the efficacy of chondrocytes within their limited life span. To that end, the combination of exogenous growth factors with mechanical stimuli holds promise for development of clinically relevant cartilage tissue substitutes. The current study aimed to determine whether incorporation of transient exposure to growth factors into a hydrodynamic bioreactor system can improve the functional maturation of tissue-engineered cartilage. Chondrocyte-seeded polyglycolic acid scaffolds were cultivated within a wavy-walled bioreactor that imparts fluid flow-induced shear stress for 4 weeks. Constructs were nourished with 100 ng/mL insulin-like growth factor-1 (IGF-1) or 10 ng/mL transforming growth factor-b1 (TGF-b1) either for the first 15 days of the culture (transient) or throughout the entire cultivation (continuous). Transiently treated constructs were found to exhibit better functional properties than continuously nourished constructs. The limited development of engineered tissues continuously stimulated by IGF-1 or TGF-b1 was related to massive growth factor leftovers in the environments that downregulated the expression of the associated receptors. Treatment with TGF-b1 eliminated the formation of a fibrous capsule at the construct periphery possibly through suppression of Smad3 phosphorylation, yielding constructs with greater homogeneity. Furthermore, TGF-b1 reversely regulated Smad2 and Smad3 pathways in articular chondrocytes under hydrodynamic stimuli partially via Smad7. Collectively, transient exposure to growth factors is likely to maintain chondrocyte homeostasis, and thus promotes their anabolic activities under hydrodynamic stimuli. The present work suggests that robust hydrodynamically engineered neocartilage with a reduced fibrotic response and enhanced tissue homogeneity can be achieved through optimization of growth factor supplementation protocols and potentially through manipulation of intracellular signals such as Smad.

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Enhancing cell seeding of scaffolds in tissue engineering through manipulation of hydrodynamic parameters

Cited by 60 publications

References 41 publications

Cationic Surface Charge Combined with Either Vitronectin or Laminin Dictates the Evolution of Human Embryonic Stem Cells/Microcarrier Aggregates and Cell Growth in Agitated Cultures

Cationic Surface Charge Combined with Either Vitronectin or Laminin Dictates the Evolution of Human Embryonic Stem Cells/Microcarrier Aggregates and Cell Growth in Agitated Cultures

Carbon nanotube interaction with extracellular matrix proteins producing scaffolds for tissue engineering

Differential Morphology and Homogeneity of Tissue-Engineered Cartilage in Hydrodynamic Cultivation with Transient Exposure to Insulin-Like Growth Factor-1 and Transforming Growth Factor-β1

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