Enhancing cellulases through synergistic β-glucosidases for intensifying cellulose hydrolysis

Fernandes, Custan G.; Sawant, Sneha; Mule, Tejal A.; Khadye, Vishwanath S.; Lali, Arvind; Odaneth, Annamma A.

doi:10.1016/j.procbio.2022.06.011

Cited by 15 publications

(3 citation statements)

References 32 publications

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“…The test results showed that the strains used in our experiment had the potential to degrade cellulose, indicating that the tested strains could degrade cellulose. Studies have shown that the combined action of these three enzymes is more effective in degrading cellulose (Fernandes et al, 2022;Krisztina et al, 2022). Our findings showed that three L. plantarum strains used in the project had low cellulose degradation potential which appears to be further reduced over fermentation time.…”

Section: Discussionmentioning

confidence: 99%

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A study revealing mechanisms behind the stone cell of Yali pear degradation by mixed‐culture fermentation of lactic acid bacteria and yeast

Gao,

Xu,

Tian

et al. 2024

eFood

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The presence of stone cells in pears is recognized as a problem for the pear processing industry. Bacillus and mold can degrade stone cells because of their potential to digest cellulose and lignin, but they cannot be used for the degradation test of pear stone cells. In this study Lactoplantibacillus plantarum JYLP‐326 was used in single culture or in the mixed culture with Saccharomyces cerevisiae Y2 to study their potential to degrade stone cells during fermentation and the mechanism of degradation was further explored. Synergy in cellulase activity was observed in the mixed‐culture where the maximum activity was observed at 96th hour of fermentation. Activities of endoglucanase, exoglucanase and β‐glucosidase were 1.75, 4.58, and 2.31. The degradation rate of stone cells in the mixed‐culture was 37.67%, which was significantly higher than that the results obtained for single cultures. The results of scanning electron microscopy (SEM) showed that the surface of the cultured stone cells became rough. Metabolomics studies confirmed the presence of specific metabolites related to the degradation of stone cells after the fermentation. It was concluded that the mixed‐culture fermentation using the above‐mentioned strains could be exploited by the pear processing industry to degrade stone cells.

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Section: Discussionmentioning

confidence: 99%

“…Studies have shown that the combined action of these three enzymes is more effective in degrading cellulose (Fernandes et al, 2022; Krisztina et al, 2022). Our findings showed that three L. plantarum strains used in the project had low cellulose degradation potential which appears to be further reduced over fermentation time.…”

Section: Discussionmentioning

confidence: 99%

A study revealing mechanisms behind the stone cell of Yali pear degradation by mixed‐culture fermentation of lactic acid bacteria and yeast

Gao,

Xu,

Tian

et al. 2024

eFood

View full text Add to dashboard Cite

show abstract

“…In order to obtain an efficient cocktail, some parameters must be taken into account, such as microorganisms’ behavior when cultivated on different substrates, identifying the types of enzymes they produce in each case, and which enzymes are necessary for every kind of substrate degradation [ 60 ]. Various approaches can be used for enzymatic cocktails production, such as combinations of distinct species of fungi [ 248 , 249 , 250 , 251 ], bacteria [ 252 , 253 , 254 ], or even fungi and bacteria [ 255 , 256 , 257 ]. Furthermore, strategies for optimizing an enzymatic cocktail may include supplementation with a specific enzyme, be it hydrolytic or not, or supplementation with surfactants or other chemicals [ 247 ].…”

Section: Lignocellulolytic Enzymes Productionmentioning

confidence: 99%

Lignocellulolytic Biocatalysts: The Main Players Involved in Multiple Biotechnological Processes for Biomass Valorization

Benatti

Polizeli

2023

Microorganisms

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Human population growth, industrialization, and globalization have caused several pressures on the planet’s natural resources, culminating in the severe climate and environmental crisis which we are facing. Aiming to remedy and mitigate the impact of human activities on the environment, the use of lignocellulolytic enzymes for biofuel production, food, bioremediation, and other various industries, is presented as a more sustainable alternative. These enzymes are characterized as a group of enzymes capable of breaking down lignocellulosic biomass into its different monomer units, making it accessible for bioconversion into various products and applications in the most diverse industries. Among all the organisms that produce lignocellulolytic enzymes, microorganisms are seen as the primary sources for obtaining them. Therefore, this review proposes to discuss the fundamental aspects of the enzymes forming lignocellulolytic systems and the main microorganisms used to obtain them. In addition, different possible industrial applications for these enzymes will be discussed, as well as information about their production modes and considerations about recent advances and future perspectives in research in pursuit of expanding lignocellulolytic enzyme uses at an industrial scale.

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Technological Advancements in Enzyme Production for 2G Ethanol

Sukumaran,

Sankar,

Adarsh

et al. 2024

Value Addition and Product Diversification in Sugarcane

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Enhancing cellulases through synergistic β-glucosidases for intensifying cellulose hydrolysis

Cited by 15 publications

References 32 publications

A study revealing mechanisms behind the stone cell of Yali pear degradation by mixed‐culture fermentation of lactic acid bacteria and yeast

A study revealing mechanisms behind the stone cell of Yali pear degradation by mixed‐culture fermentation of lactic acid bacteria and yeast

Lignocellulolytic Biocatalysts: The Main Players Involved in Multiple Biotechnological Processes for Biomass Valorization

Technological Advancements in Enzyme Production for 2G Ethanol

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