Enhancing Design of Immobilized Enzymatic Microbioreactors Using Computational Simulation

Bailey, Robert T.; Jones, Frank; Fisher, Ben; Elmore, Bill B.

doi:10.1007/978-1-59259-991-2_54

Cited by 2 publications

(3 citation statements)

References 11 publications

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“…For modeling microbioreactors, where the intraparticle and external diffusion resistance are significant, multicompartment models are required to accomplish adequate model accuracy [35]. However, monocompartment models, in which the internal mass transport by diffusion and substrate conversion is considered, are still used in various applications due to the simplicity of the model [40]. In addition, substrate conversion is often studied only when enzyme kinetics approach first-or zero-order kinetics [33,41].…”

Section: Kineticsmentioning

confidence: 99%

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10 Continuous-flow biocatalysis with enzymes and cells

Paradisi¹,

Poppe²

2021

Flow Chemistry – Applications

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The advantages of synthetic chemistry performed in continuous flow synergize with the benefits conferred by biocatalysis, including reactions with greener, milder, lower temperatures, and aqueous conditions. Furthermore, the fine control over reaction conditions in continuous flow can solve inherent challenges associated with catalysis by enzymes, such as substrate and product inhibition. Cells and enzymes also benefit from the improved mixing, mass transfer, thermal control, pressurized processing, decreased variation, automation, along with in-line product analysis and purification conferred through continuous flow. Thus, the combination of continuous flow and biocatalysis has emerged as a highly effective approach for creating diverse synthetic targets. Examples include immobilized enzymes and whole cells in continuous flow for the synthesis of pharmaceuticals, chemicals, and materials. Challenges to continuous-flow biocatalysis surveyed in this chapter include immobilization methods, the necessity to regenerate cofactors for cell-free biocatalysis, and optimization of biosynthetic steps with highly divergent conditions in flow.

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Section: Kineticsmentioning

confidence: 99%

“…The simulation approach allows to estimate certain kinetic parameters and to optimize the microfluidic reactor system with significantly decreased time and cost [35,39,40,42].…”

Section: Kineticsmentioning

confidence: 99%

10 Continuous-flow biocatalysis with enzymes and cells

Paradisi¹,

Poppe²

2021

Flow Chemistry – Applications

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“…This has led to extensive studies of the behavior in microscale fluidic channels. Reaction behavior in these channels was physically studied and later simulated using CFD-ACE+ computational software (6). Investigators are striving to acquire the ability to fully use the inherent advantages of the small scale.…”

Section: Introductionmentioning

confidence: 99%

The effects of engineering design on heterogeneous biocatalysis in microchannels

Jones

Bailey

Wilson

et al. 2007

Appl Biochem Biotechnol

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The results of a numerical study of the fundamental interactions of engineering design and micromixing on conversion in packed microchannels are presented. Previously, channel-based microreactors made of molded silicon plastic were designed, fabricated, and experimentally tested. These reactors have enzymes immobilized on the channel walls by various methods including layer-by-layer nano self-assembly techniques. They also contain molded packing features to add reactive surface area and to redistribute the fluid. An arbitrary but intuitively sensible packing arrangement was initially chosen and used in experimental studies. The current computer simulation study was undertaken to understand how static laminar mixing affects the conversion efficiency. The reactors previously used experimentally have been simulated using CFD-ACE+multiphysics software (ESI CFD Inc., Huntsville, AL). It is found that packing significantly increases conversion when compared with empty channels over the entire flow rate range of the study (0.25

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Enhancing Design of Immobilized Enzymatic Microbioreactors Using Computational Simulation

Cited by 2 publications

References 11 publications

10 Continuous-flow biocatalysis with enzymes and cells

10 Continuous-flow biocatalysis with enzymes and cells

The effects of engineering design on heterogeneous biocatalysis in microchannels

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