Enhancing Effect of Manganese on L‐DOPA‐Induced Apoptosis in PC12 Cells

Migheli, Rossana; Godani, C; Sciola, L.; Delogu, Maria Rosaria; Serra, Pier Andrea; Zangani, Danilo; Natale, G. De; Miele, E; Desole, Maria Speranza

doi:10.1046/j.1471-4159.1999.0731155.x

Cited by 94 publications

(67 citation statements)

References 38 publications

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“…L-DOPA at concentrations higher than 100 µM also produces the intracellular cytotoxicity for 24 h, which is mediated by oxidative stress (Migheli, 1999;Lee et al, 2003). In this study, harman (10-30 µM) and norharman (50-150 µM) reduced L-DOPA (20-50 µM)-induced increases of dopamine content for 24 h in PC12 cells.…”

Section: Discussionmentioning

confidence: 56%

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The Harman and Norharman Reduced Dopamine Content and Induced Cytotoxicity in PC12 Cells

Yang¹,

Lim²,

Lee³

2008

Biomolecules and Therapeutics

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-The effects of harman and norharman on dopamine content and L-DOPA-induced cytotoxicity were investigated in PC12 cells. Harman and norharman decreased the intracellular dopamine content for 24 h. The IC 50 values of harman and norharman were 20.4 µM and 95.8 µM, respectively. Tyrosine hydroxylase (TH) activity and TH mRNA levels were also decreased by 20 µM harman and 100 µM norharman. Under the same conditions, the intracellular cyclic AMP levels were decreased by harman and norharman. In addition, harman and norharman at concentrations higher than 80 µM and 150 µM caused cytotoxicity at 24 h in PC12 cells. Non-cytotoxic ranges of 10-30 µM harman and 50-150 µM norharman inhibited L-DOPA (20-50 µM)-induced increases of dopamine content at 24 h. Harman at 20-150 µM and norharman at 100-300 µM also enhanced L-DOPA (20-100 µM)-induced cytotoxicity at 24 h. These results suggest that harman and norharman decrease dopamine content by reducing TH activity and aggravate L-DOPA-induced cytotoxicity in PC12 cells.

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Section: Discussionmentioning

confidence: 56%

“…L-DOPA at 20-100 µM increases dopamine content after 24 or 48 h of incubation in PC12 cells (Migheli, 1999;Lee et al, 2003). L-DOPA at concentrations higher than 100 µM also produces the intracellular cytotoxicity for 24 h, which is mediated by oxidative stress (Migheli, 1999;Lee et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

The Harman and Norharman Reduced Dopamine Content and Induced Cytotoxicity in PC12 Cells

Yang¹,

Lim²,

Lee³

2008

Biomolecules and Therapeutics

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“…L-DOPA treatment in MPTP-induced PD rodents increased the striatal 6-OHDA levels, which may be sensitized by monoamine oxidase inhibitor (Borah & Mohanakumar, 2010). Long-term treatment with L-DOPA caused disabling motor side effects in PD and alleviated oxidative stress-induced neurotoxicity by ROS formation against striatal dopaminergic neurons and PC12 cells (Basma et al, 1995;Walkinshaw & Waters, 1995;Migheli et al, 1999). Subchronic or chronic L-DOPA treatment resulted in increased levels of dopamine and hydroxyl-free radicals in the striatum (Pandey et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective Effects of Herbal Butanol Extracts from Gynostemma pentaphyllum on the Exposure to Chronic Stress in a 6-Hydroxydopamine- Lesioned Rat Model of Parkinson's Disease Treated with or Without L-DOPA

Lee¹,

Choi²,

Chen³

et al. 2012

Mechanisms in Parkinson's Disease - Models and Treatments

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“…Chromatographic analysis of dialysates from PC12 cell suspension DA was quantified in dialysates of selected experiments (1.5 Â 10 6 cells) by HPLC-EC, as described previously [6][7][8]19 …”

Section: Methodsmentioning

confidence: 99%

“…A fluid mixer (FM) (a and b in Fig. 5, top left schematic) was made using one 23-G needle (a) and 15 mm of plastic-coated silica tubing (b, CAL input in Cell culture PC12 cells were maintained at 37°C in 60-mm plastic culture plates in a humidified atmosphere of 5% CO 2 /95% air as described previously [19]. The formulation of the culture medium was as follows: Dulbecco's modified Eagle's medium (DMEM)-F12 supplemented with 10% horse serum and 5% fetal calf serum.…”

Section: Drug Reservoir and Fluid Mixermentioning

confidence: 99%

Novel integrated microdialysis–amperometric system for in vitro detection of dopamine secreted from PC12 cells: Design, construction, and validation

Migheli¹,

Puggioni²,

Dedola³

et al. 2008

Analytical Biochemistry

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a b s t r a c tA novel dual channel in vitro apparatus, derived from a previously described design, has been coupled with dopamine (DA) microsensors for the flow-through detection of DA secreted from PC12 cells. The device, including two independent microdialysis capillaries, was loaded with a solution containing PC12 cells while a constant phosphate-buffered saline (PBS) medium perfusion was carried out using a dual channel miniaturized peristaltic pump. One capillary was perfused with normal PBS, whereas extracellular calcium was removed from extracellular fluid of the second capillary. After a first period of stabilization and DA baseline recording, KCl (75 mM) was added to the perfusion fluid of both capillaries. In this manner, a simultaneous ''treatment-control" experimental design was performed to detect K + -evoked calcium-dependent DA secretion. For this purpose, self-referencing DA microsensors were developed, and procedures for making, testing, and calibrating them are described in detail. The electronic circuitry was derived from previously published schematics and optimized for dual sensor constant potential amperometry applications. The microdialysis system was tested and validated in vitro under different experimental conditions, and DA secretion was confirmed by high-performance liquid chromatography with electrochemical detection (HPLC-EC). PC12 cell viability was quantified before and after each experiment. The proposed apparatus serves as a reliable model for studying the effects of different drugs on DA secretion through the direct comparison of extracellular DA increase in treatment-control experiments performed on the same initial PC12 cell population.Ó A wide variety of techniques have been used for studying in vitro DA secretion/release, and most of them use PC12 cells because they are capable of synthesizing, secreting, and metabolizing DA. The PC12 cell line, obtained from rat pheochromocytoma of the adrenal medulla, is also used as a tool to understand the biochemical mechanisms underlying the physiology and degeneration of central dopamine neurons [12][13][14]. In previous studies, we successfully used PC12 cells to investigate the mechanism of nitric oxide (NO) donor-induced DA secretion [7] and DA oxidative homeostasis in vitro [6,8]. In such studies, DA was quantified in microdialysates by high-performance liquid chromatography with electrochemical detection (HPLC-EC). The aim of the current research was to combine a modified device for in vitro microdialysis with a novel, dual channel amperometric system for the online detection of DA secreted from PC12 cells. The new electronics de-0003-2697/$ -see front matter Ó

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Enhancing Effect of Manganese on L‐DOPA‐Induced Apoptosis in PC12 Cells

Cited by 94 publications

References 38 publications

The Harman and Norharman Reduced Dopamine Content and Induced Cytotoxicity in PC12 Cells

The Harman and Norharman Reduced Dopamine Content and Induced Cytotoxicity in PC12 Cells

Neuroprotective Effects of Herbal Butanol Extracts from Gynostemma pentaphyllum on the Exposure to Chronic Stress in a 6-Hydroxydopamine- Lesioned Rat Model of Parkinson's Disease Treated with or Without L-DOPA

Novel integrated microdialysis–amperometric system for in vitro detection of dopamine secreted from PC12 cells: Design, construction, and validation

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