Enhancing frequency of regeneration of somatic embryos of avocado (Persea americana Mill.) using semi-permeable cellulose acetate membranes

Palomo-Ríos, Elena; Perez, C Alexander; Mercado, José A.; Pliego‐Alfaro, Fernando

doi:10.1007/s11240-013-0352-9

Cited by 9 publications

(7 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Generally, the accumulation of storage products turns translucent embryos into white-opaque embryos. This change has been used as a maturity indicator in several species, including cork oak (Pintos et al 2010), olive (Cerezo et al 2011) and avocado (Palomo-Ríos et al 2013). The data presented here revealed that sucrose concentration was another important factor controlling SSE development, although different trends in response to this variable were shown by the two oak species.…”

Section: Discussionmentioning

confidence: 68%

Improved secondary embryo production in Quercus alba and Q. rubra by activated charcoal, silver thiosulphate and sucrose: influence of embryogenic explant used for subculture

Martínez

Viéitez

Corredoira

2015

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

In somatic embryogenic systems of various oak species, including Quercus alba and Q. rubra, embryogenic lines generally proliferate through generation of nodular embryogenic structures (NS) as a form of repetitive embryogenesis, without embryo development progressing to the cotyledonary stage, or with a low proportion of embryo formation, resulting in the inability to achieve suitable material for plant recovery. In experiments we used previously initiated embryogenic lines derived from 7-year-old trees of Q. alba and Q. rubra. The aim of this work was to improve embryo proliferation and histodifferentiation of lines from these oak species to obtain welldeveloped embryos and plant regeneration. The effects of activated charcoal, silver thiosulphate (STS) and sucrose concentration on secondary embryo production were investigated. Overall, the best embryo proliferation medium consisted of basal Murashige and Skoog medium containing 0.4 % charcoal and 20 lM STS, and supplemented with 6 % sucrose for Q. alba and 3 % sucrose for Q. rubra. In these conditions, well-developed and singularised torpedo-and cotyledonary-shaped embryos were produced. The developmental stage of the embryogenic explant used for subculture significantly influenced embryo production and differentiation of new secondary embryos, with NS being the most effective explant despite being the smallest explant type in terms of size and fresh weight.Addition of STS in the germination medium also had a positive effect on germination response giving rise to approximately 39 % embryo conversion in Q. alba and 11 % in Q. rubra.

show abstract

Section: Discussionmentioning

confidence: 68%

Improved secondary embryo production in Quercus alba and Q. rubra by activated charcoal, silver thiosulphate and sucrose: influence of embryogenic explant used for subculture

Martínez

Viéitez

Corredoira

2015

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…Based on these data, we conclude that only the increase of the sucrose concentration at the levels tested is not enough to reach the level of water stress needed to control the maturation of grapevine somatic embryos, which is important for the normal conversion of somatic embryos to plants, as previously shown [ 8 ]. In avocado, the use of a semipermeable cellulose membrane during the prematuration step of somatic embryos resulted in mature somatic embryos of better quality and germination ability [ 14 ].…”

Section: Resultsmentioning

confidence: 99%

“…This procedure has been previously used to control the maturation of somatic embryos in citrus and olive [ 12 , 13 ]. In avocado ( Persea americana ), the culture of embryogenic calli on top of cellulose acetate membranes increased the number of well-developed mature somatic embryos and improved their further germination [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Changes in abscisic acid metabolism in relation to the maturation of grapevine (Vitis vinifera L., cv. Mencía) somatic embryos

et al. 2020

View full text Add to dashboard Cite

Background Somatic embryogenesis in grapevines is a complex process that depends on many physiological and genetic factors. One of its main limitations is the process of precocious germination of the somatic embryos in differentiation medium. This process lowers plant conversion rates from the somatic embryos, and it is probably caused by a low endogenous abscisic acid (ABA) content. Results Precocious germination of the somatic embryos was successfully avoided by culturing grapevine cv. Mencía embryogenic aggregates over a semipermeable membrane extended on top of the differentiation medium. The weekly analysis of the endogenous ABA and ABA-glucosyl ester (ABA-GE) contents in the aggregates showed their rapid accumulation. The expression profiles of 9-cis-epoxycarotenoid dioxygenase (VvNCED1), 8′-hydroxylase (VvHyd2), UDP-glucosyltransferase (VvUGT) and β-glucosidase (VvBG2) genes in grapevine revealed that the occurrence of a first accumulation peak of endogenous ABA in the second week of culture over the semipermeable membrane was mainly dependent on the expression of the VvNCED1 gene. A second increase in the endogenous ABA content was observed in the fourth week of culture. At this point in the culture, our results suggest that of those genes involved in ABA accumulation, one (VvNCED1) was repressed, while another (VvBG2) was activated. Similarly, of those genes related to a reduction in ABA levels, one (VvUGT) was repressed while another (VvHyd2) was activated. The relative expression level of the VvNCED1 gene in embryogenic aggregates cultured under the same conditions and treated with exogenous ABA revealed the significant downregulation of this gene. Conclusions Our results demonstrated the involvement of ABA metabolism in the control of the maturation of grapevine somatic embryos cultured over a semipermeable membrane and two important control points for their endogenous ABA levels. Thus, subtle differences in the expression of the antagonistic genes that control ABA synthesis and degradation could be responsible for the final level of ABA during the maturation of grapevine somatic embryos in vitro. In addition, the treatment of somatic embryos with exogenous ABA suggested the feedback-based control of the expression of the VvNCED1 gene by ABA during the maturation of grapevine somatic embryos.

show abstract

“…By and large, BA together with 2,4-D [164] remain the most commonly used synergistic CK-auxin combination in the plant propagation of important fruit tree species such as banana, apple, grapes, litchi, sweet orange, passion fruit, avocado, and guava through somatic embryogenesis (Table 2). Moreover, plant recovery stages depend primarily on a CK-GA 3 combination [135,139,[145][146][147]. However, despite early promising results of N 6 -(3-hydroxybenzyl) adenine (meta-topolin, mT) as a possible alternative to BA in plant tissue culture [165,166] and its subsequent application in the organogenesis of numerous plant species [167], there is a dearth of information on the use of meta-topolins in the somatic embryogenesis of fruit crops.…”

Section: Influence Of Cytokinins In Micropropagation Of Horticultural Fruit Cropsmentioning

confidence: 99%

Applications of Cytokinins in Horticultural Fruit Crops: Trends and Future Prospects

et al. 2020

View full text Add to dashboard Cite

Cytokinins (CKs) are a chemically diverse class of plant growth regulators, exhibiting wide-ranging actions on plant growth and development, hence their exploitation in agriculture for crop improvement and management. Their coordinated regulatory effects and cross-talk interactions with other phytohormones and signaling networks are highly sophisticated, eliciting and controlling varied biological processes at the cellular to organismal levels. In this review, we briefly introduce the mode of action and general molecular biological effects of naturally occurring CKs before highlighting the great variability in the response of fruit crops to CK-based innovations. We present a comprehensive compilation of research linked to the application of CKs in non-model crop species in different phases of fruit production and management. By doing so, it is clear that the effects of CKs on fruit set, development, maturation, and ripening are not necessarily generic, even for cultivars within the same species, illustrating the magnitude of yet unknown intricate biochemical and genetic mechanisms regulating these processes in different fruit crops. Current approaches using genomic-to-metabolomic analysis are providing new insights into the in planta mechanisms of CKs, pinpointing the underlying CK-derived actions that may serve as potential targets for improving crop-specific traits and the development of new solutions for the preharvest and postharvest management of fruit crops. Where information is available, CK molecular biology is discussed in the context of its present and future implications in the applications of CKs to fruits of horticultural significance.

show abstract

Enhancing frequency of regeneration of somatic embryos of avocado (Persea americana Mill.) using semi-permeable cellulose acetate membranes

Cited by 9 publications

References 32 publications

Improved secondary embryo production in Quercus alba and Q. rubra by activated charcoal, silver thiosulphate and sucrose: influence of embryogenic explant used for subculture

Improved secondary embryo production in Quercus alba and Q. rubra by activated charcoal, silver thiosulphate and sucrose: influence of embryogenic explant used for subculture

Changes in abscisic acid metabolism in relation to the maturation of grapevine (Vitis vinifera L., cv. Mencía) somatic embryos

Applications of Cytokinins in Horticultural Fruit Crops: Trends and Future Prospects

Contact Info

Product

Resources

About