2003
DOI: 10.1126/science.1079512
|View full text |Cite
|
Sign up to set email alerts
|

Enhancing Gene Targeting with Designed Zinc Finger Nucleases

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1

Citation Types

7
498
1
3

Year Published

2006
2006
2021
2021

Publication Types

Select...
7
3

Relationship

1
9

Authors

Journals

citations
Cited by 757 publications
(509 citation statements)
references
References 16 publications
7
498
1
3
Order By: Relevance
“…R GENs are derived from the adaptive immune system in bacteria and archaea known as clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPRassociated protein (Cas) 1 ; they now belong to a family of genome-editing engineered nucleases that induce site-specific DNA cleavages in cells and organisms, whose repair via endogenous DNA double-strand break (DSB) repair systems gives rise to targeted mutagenesis 2,3 and chromosomal rearrangements 4,5 . Unlike zinc-finger nucleases (ZFNs) or transcription activator-like effector (TALE) nucleases (TALENs), whose DNA sequence specificities are determined by protein moieties, RGENs recognize and cleave DNA in a targeted manner using two separate components, that is, a small RNA component termed CRISPR RNA (crRNA) or single-chain guide RNA (sgRNA) that hybridizes with a 20-base pair (bp) long target DNA sequence and the Cas9 protein, derived from Streptococcus pyogenes, which recognizes the NGG (or, to a lesser extent, NAG 6 ) trinucleotide sequence known as the protospacer-adjacent motif (PAM).…”
mentioning
confidence: 99%
“…R GENs are derived from the adaptive immune system in bacteria and archaea known as clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPRassociated protein (Cas) 1 ; they now belong to a family of genome-editing engineered nucleases that induce site-specific DNA cleavages in cells and organisms, whose repair via endogenous DNA double-strand break (DSB) repair systems gives rise to targeted mutagenesis 2,3 and chromosomal rearrangements 4,5 . Unlike zinc-finger nucleases (ZFNs) or transcription activator-like effector (TALE) nucleases (TALENs), whose DNA sequence specificities are determined by protein moieties, RGENs recognize and cleave DNA in a targeted manner using two separate components, that is, a small RNA component termed CRISPR RNA (crRNA) or single-chain guide RNA (sgRNA) that hybridizes with a 20-base pair (bp) long target DNA sequence and the Cas9 protein, derived from Streptococcus pyogenes, which recognizes the NGG (or, to a lesser extent, NAG 6 ) trinucleotide sequence known as the protospacer-adjacent motif (PAM).…”
mentioning
confidence: 99%
“…Meganucleases such as I-SceI are valuable cleavage reagents because of their long recognition sites (19-21), but their sites must be introduced before they can be attacked. Because zinc fingers exist for many of the DNA triplets, nucleases of this type can theoretically be created to target almost any preexisting locus.Studies performed in Drosophila and in human cells have shown that ZFNs can be designed to effectively cleave specific sequences (22)(23)(24)(25)(26)(27)(28). Additional studies with synthetic targets have demonstrated the efficiency of ZFN-directed cleavage in Xenopus, in mammalian cells and in plants (16,(29)(30)(31).…”
mentioning
confidence: 99%
“…It is also possible to hijack the repair of the DSB induced by a ZFN or TALEN to introduce alternations (point mutations, deletions) at the cut site [61]. From their beginnings in Drosophila [62], ZFNs have recently been used to perform targeted mutagenesis in mouse zygotes (one-cell embryos) ( Figure 1C) [63,64]. Modification of somatic cells in vivo and gene correction at the haemophilia B locus have also been achieved using ZFNs [65].…”
Section: Engineered Nucleases and Tools For Genome Editingmentioning
confidence: 99%