Enhancing In Vitro Multiplication of some Olive Cultivars Using Silver, Selenium And Chitosan Nanoparticles

Abstract

Olive trees are commercially propagated by cuttings or grafting on clonal or seedling rootstocks; recently, olive micropropagation has emerged as a powerful technique for mass production of true to type and pathogen free plants. The current study was carried out to evaluate the effect of silver, chitosan and selenium nanoparticles as microbial decontamination agents, as well as evaluate its possible effects on in vitro shoot growth and multiplication of three olive cultivars namely Koroneiki, Picual and Manzanillo. Validation and characterization of the biosynthesized nanoparticles was carried out by Transmission Electron Microscopy. The produced nanoparticles were added to the olive culture medium, and the growth parameters were determined. The tested nanoparticles showed varied degree of antimicrobial activity, silver nanoparticles were highly effective to inhibit in vitro microbial contamination. The effect of genotypes on shoot growth and multiplication was significant; Koroneiki and Picual cultivars showed better growth measurements compared with Manzanillo. The addition of nanoparticles to the culture medium had a significant outcome on growth and multiplication rate of in vitro growing olive shoots. Silver nanoparticles produced higher values of number of shoots, shoots length, leaf number and multiplication rate compared with the other treatments. In conclusion; the current results showed that nanoparticles were efficient as in vitro disinfectant agent and the nanoparticles addition to culture medium especially silver NPs had a positive effect on growth and multiplication rate of in vitro growing olive shoots.

Genetic Transformation of two Turkish rice (Oryza sativa L.) cutivars through Agrobacterium tumefaciens

“…It is very important to determine the most effective and the lowest disinfection dose for the surface sterilization of an explant [37]. Although several chemicals like hydrogen peroxide, mercury, silver nitrate, and antibiotics can be used for seed surface sterilization, commercial sodium hypochlorite (bleach) is commonly used for the purpose [38,39]; yet the NaOCl is the most preferred disinfectant used World over [40, 41,], this study showed no signi cant distinction in seed germination behavior of the two rice cultivars. and showed that NaOCl-based seed disinfection was rice seed friendly [42].…”

Exposure of Caralluma tuberculata to biogenic selenium nanoparticles as in vitro rooting agent: Stimulates morpho-physiological and antioxidant defense system

“…In a study conducted by Darwesh and colleagues in 2023, an investigation was undertaken to assess the impact of silver, chitosan, and selenium nanoparticles on the in vitro growth of plantlets from three distinct olive cultivars: Koroneiki, Picual, and Manzanillo. The introduction of nanoparticles into the growth medium yielded noteworthy effects on the proliferation and growth rate of in vitro olive plants [44]. Therefore, we may suggest that phyto-mediated SeNPs elicited the rooting development of C. tuberculata by interfering with endogenous auxin…”

Anticancer Activity of the in Vitro-Derived Extract of Cactus (Opuntia ficus-indica L. Mill) Treated with Selenium Nanoparticles

“…Selenium nanoparticle solutions at two different concentrations (2.5 and 5 mg l −1 ) were obtained through personal communication with Professor Osama Darwish. Both solutions have been previously prepared by Darwish et al [10]. Stock solutions of both 100 mM sodium selenite and 50 mM ascorbic acid were prepared using sterile deionized water.…”