Enhancing Lipid Production of Dunaliella tertiolecta by Manipulating the Interactive Effect of Salinity and Nitrogen

Rizwan, Muhammad; Mujtaba, Ghulam; Rashid, Naim; Lee, K.

doi:10.15255/cabeq.2017.1092

Cited by 9 publications

(5 citation statements)

References 29 publications

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“…Marine microalgae can utilise inorganic nitrogen for their growth and metabolic activities and increase their biomass concentrations in conjunction with the nitrogen-enriched condition (Rizwan et al, 2017). The present study findings also revealed that the increment of nitrogen (NaNO 3 ) supply in the medium significantly increased biomass production of T. chuii, but the contrary result was found for decreased concentrations of nitrogen.…”

Section: Resultssupporting

confidence: 58%

Effects of sodium nitrate on the growth and proximate composition of the indigenous marine microalgae Tetraselmis chuii (Butcher, 1959)

Barman¹,

Khatoon²,

Rahman³

et al. 2021

ase

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Nitrogen is one of the fundamental nutrients for algal growth, underpinning the microalgal biochemical composition. Therefore, this study compared the growth and proximate compositions of Tetraselmis chuii (Butcher, 1959), cultured in different nitrate (NaNO 3 ) concentrations (25, 50, 100, 200 and 500 mg L -1 ). Thus, the cell density, optical density, specific growth rate and division rate of T. chuii were measured daily. Furthermore, protein and carbohydrate contents were also determined in the stationary phase. The results showed that T. chuii cultivated in a NaNO 3 concentration of 500 mg L -1 had significantly (p<0.05) higher growth in terms of cell density, biomass, optical density, specific growth rate and division rates compared with other concentrations. Likewise, protein content was also significantly higher under the NaNO 3 concentration of 500 mg L -1 , whereas significantly (p<0.05) higher carbohydrate content was found at 25 mg L -1 NaNO 3 compared with the other concentrations, showing a contrary trend between protein and carbohydrate concentrations, respectively. Since the primary focus has been on improving the quality of microalgal biomass in order to develop novel processes and products, this is the first study to use higher concentrations of modified nitrate on T. chuii isolated from the coastal area of Bangladesh. Thus the indigenous marine T. chuii had significantly utilised NaNO 3 concentrations with higher growth and proximate contents in this study. However, further study is needed on microalgal genetics and metabolic engineering to create a new molecular era of the indigenous marine microalgae isolated from the coastal water of Bengal.

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Section: Resultssupporting

confidence: 58%

Effects of sodium nitrate on the growth and proximate composition of the indigenous marine microalgae Tetraselmis chuii (Butcher, 1959)

Barman¹,

Khatoon²,

Rahman³

et al. 2021

ase

View full text Add to dashboard Cite

show abstract

“…Jahnke and White (2003) demonstrated that while different Dunaliella species can grow in a wide range of salinities (2.9 -175 PSU) only D. tertiolecta can grow in salinity lower than 3 PSU, as we also observed here. A decrease in D. tertiolecta growth under hyposalinity was previously observed (Jahnke and White 2003;Rizwan et al 2017). In addition, hyposalinity was found to lead to an increase in D. tertiolecta cell size, which had reduced F v /F m levels and a smaller ascorbate pool along with an increased pool of α-tocopherol and total glutathione (Jahnke and White 2003).…”

Section: Cell Growth In Response To Salinity Decreasesupporting

confidence: 59%

Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae)

et al. 2022

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Marine microalgae often live in a fluctuating environment including a decrease in salinity caused by global warming induced sea ice melting and freshwater inflows. Under conditions of fluctuating salinity, microalgae have evolved a variety of survival mechanisms such as lipid accumulation and remodeling. The purpose of this study was to investigated the membrane lipid remodeling of the marine green microalga Dunaliella tertiolecta as a short-term acclimation mechanism in response to hyposalinity (20 and 3 PSU) with respect to growth at optimal salinity (38 PSU). We identified 34 lipid species belonging to seven polar lipid classes. Dunaliella tertiolecta accumulates cell lipids and remodels polar lipid classes and their fatty acids composition as response to hypoosmotic stress at 3 PSU. We found that the unsaturation of most polar lipids decreases overall, indicating decreased membrane fluidity and altered permeability, whereas shortening the length of fatty acids of polar lipids is not one of the strategies of D. tertiolecta to cope with the decrease in salinity. Increase in relative content (%) and unsaturation of monogalactosyldiacylglycerols (MGDG) and decrease in relative content (%) and unsaturation of phosphatidylglycerols (PG), suggesting changes in photosynthetic membranes of thylakoids at 20 and 3 PSU. At a very low salinity of 3 PSU, the relative content (%) of phosphatidylinositols (PI) increases, suggesting increased lipid trafficking and signaling in the cells. These changes are statistically significant and we hypothesize that D. tertiolecta is genetically adapted to withstand large salinity fluctuations through polar lipid composition.

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“…7 A similar pattern of lipid accumulation at intermediate salinity was also described in the halotolerant Dunaliella tertiolecta. 92 Interestingly, in the halophile Dunaliella salina, cell lipid content increased with salinity up to 2 M NaCl. 50 The fluctuating response of total lipid between close strains may be partly influenced by the existence of high intraspecific variability 93 and eventual evolutionary adaptations to specific salinity niches.…”

Section: Theinter Ac Tingeffec Tof Salinit Yandculturecond Iti On Son Lipidcontentinmi Croalg Aementioning

confidence: 98%

An appraisal of the variable response of microalgal lipids to culture salinity

Cañavate

Fernández‐Díaz

2021

Reviews in Aquaculture

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Salinity is an important factor in microalgal mass production that has been less studied than other external factors such as temperature and irradiance. The greater attention paid to the effects of these two factors reflects the important influence that both exert on growth and production of microalgae. 1 However, the ability to control the effects of temperature and irradiance on microalgal production is inversely proportional to the size of the culture system. This especially affects outdoor mass production systems, under which conditions the options for regulating the effects of temperature and irradiance are very limited. Therefore, lower-cost outdoor open production systems are highly dependent on how environmental factors change. In this type of system, salinity takes on a key role as an easier factor to handle to optimize production. Production of freshwater microalgae at higher salinity is possible thanks to the availability of several salttolerant species. [2][3][4][5] Microalgal production in environments of variable salinity may be even more feasible if not only estuarine species

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Enhancing Lipid Production of Dunaliella tertiolecta by Manipulating the Interactive Effect of Salinity and Nitrogen

Cited by 9 publications

References 29 publications

Effects of sodium nitrate on the growth and proximate composition of the indigenous marine microalgae Tetraselmis chuii (Butcher, 1959)

Effects of sodium nitrate on the growth and proximate composition of the indigenous marine microalgae Tetraselmis chuii (Butcher, 1959)

Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae)

An appraisal of the variable response of microalgal lipids to culture salinity

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