Enhancing Luminol Electrochemiluminescence by Combined Use of Cobalt-Based Metal Organic Frameworks and Silver Nanoparticles and Its Application in Ultrasensitive Detection of Cardiac Troponin I

Abstract

Electrochemiluminescence (ECL) has emerged as one of the most important methods for in vitro diagnosis and detection, but it is still limited in sensitivity for ultrasensitive biodetections. Fast and ultrasensitive detection of biomolecules is critical, especially for the clinical detection of cardiac troponin I (cTnI) for cardiac infarction diagnosis. In this study, an effective tactic was developed to enhance ECL efficiency of the luminol system, by combined use of Co 2+ -based metal organic frameworks (MOF), zeolitic imidazolate frameworks (ZIF-67), and luminolcapped Ag nanoparticles (luminol-AgNPs). The integration leads to a pronounced ∼115-fold enhancement in luminol ECL. On the basis of this fascinating sensing platform, a robust label-free ECL immunosensor was constructed for ultrasensitive detection of cTnI, the main marker of myocardial infarction, with good stability and a detection limit as low as 0.58 fg mL −1 (S/N = 3).

Nanoscale Technologies in Highly Sensitive Diagnosis of Cardiovascular Diseases

“…Recently, various research groups have made great progress in CVD diagnosis using ECL assay. Wang et al (2019) combined Co 2+ -based metal organic frameworks (MOF), zeolitic imidazolate frameworks (ZIF-67), and luminol-capped Ag nanoparticles (luminol-AgNPs) for fast and ultrasensitive detection of cTnI, with a 0.58 fg/mL detection limit. Zou et al (2019) developed an ECL immunosensor to detect MB in human serum.…”

A Versatile Optoelectronic Tweezer System for Micro-Objects Manipulation: Transportation, Patterning, Sorting, Rotating and Storage

“…The observation and manipulation of objects at the micro and nano scales is also one of the core technologies to study the micro/nano-science [1]. Accurate, rapid, and nondestructive manipulation of micro/nanoparticles is the basis for a range of applications such as biomedicine [2,3] and micro/nano-fabrication [4][5][6]. A number of micro-/nano-scaled methods have been proposed to manipulate [7][8][9] and fabricate micro/nanomaterials [10], including acoustic [11], optical tweezers [12,13], electrokinetic [14], magnetic [15][16][17] tweezers, and microrobot-assisted [18].…”

A Novel Graphene-Based Nanomaterial Modified Electrochemical Sensor for the Detection of Cardiac Troponin I

“…The radioimmunoassay is equal to the advantages due to its good utility and specificity, as well as its high sensitivity. However, the method has disadvantages such as the existence of radioactive contamination and short half-life of isotopes, which to a certain extent limit the development and application of radioimmunoassay (Qiao et al, 2018;Sarangadharan et al, 2018;Fu et al, 2019a;Wang et al, 2019;Mokhtari et al, 2020;Xu et al, 2020). Fluorescent immunoassay is mainly used for the diagnosis of infectious diseases due to its high sensitivity, and it is one of the oldest labeled immunoassays.…”