Enhancing Metagenomics Investigations of Microbial Interactions with Biofilm Technology

McLean, Robert; Kakirde, Kavita S.

doi:10.3390/ijms141122246

Cited by 17 publications

(9 citation statements)

References 74 publications

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“…Other novel methods for biofilm detection studies, including metagenomics and metatranscriptomics, can shed light on the complex interactions within a biofilm community ( McLean and Kakirde, 2013 ; Jahid and Sang-Do, 2014 ; Mukherjee et al, 2016 ). For example, S. aureus subspecies typing was possible using the multiple locus variable number of tandem repeats analysis (MLVA) in samples of food industry products.…”

Section: Methods For Controlling Biofilm Formation In the Food Industmentioning

confidence: 99%

Biofilms in the Food Industry: Health Aspects and Control Methods

et al. 2018

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Diverse microorganisms are able to grow on food matrixes and along food industry infrastructures. This growth may give rise to biofilms. This review summarizes, on the one hand, the current knowledge regarding the main bacterial species responsible for initial colonization, maturation and dispersal of food industry biofilms, as well as their associated health issues in dairy products, ready-to-eat foods and other food matrixes. These human pathogens include Bacillus cereus (which secretes toxins that can cause diarrhea and vomiting symptoms), Escherichia coli (which may include enterotoxigenic and even enterohemorrhagic strains), Listeria monocytogenes (a ubiquitous species in soil and water that can lead to abortion in pregnant women and other serious complications in children and the elderly), Salmonella enterica (which, when contaminating a food pipeline biofilm, may induce massive outbreaks and even death in children and elderly), and Staphylococcus aureus (known for its numerous enteric toxins). On the other hand, this review describes the currently available biofilm prevention and disruption methods in food factories, including steel surface modifications (such as nanoparticles with different metal oxides, nanocomposites, antimicrobial polymers, hydrogels or liposomes), cell-signaling inhibition strategies (such as lactic and citric acids), chemical treatments (such as ozone, quaternary ammonium compounds, NaOCl and other sanitizers), enzymatic disruption strategies (such as cellulases, proteases, glycosidases and DNAses), non-thermal plasma treatments, the use of bacteriophages (such as P100), bacteriocins (such us nisin), biosurfactants (such as lichenysin or surfactin) and plant essential oils (such as citral- or carvacrol-containing oils).

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Section: Methods For Controlling Biofilm Formation In the Food Industmentioning

confidence: 99%

Biofilms in the Food Industry: Health Aspects and Control Methods

et al. 2018

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“…Biofilm‐forming microorganisms can change their phenotype and genotype, and a viable but nonculturable state of microorganisms can lead to misinterpretation of results (reviewed in Trevors ). To solve this problem, metagenomics of pyrosequencing and Illumina‐based sequencing form better alternatives to study the mixed‐species present in food niches and their fitness in unknown samples using culture‐independent methods (reviewed in McLean and Kakirde ). Quantitative PCR based on SYBR Green I fluorescence along with microorganism‐specific primers is another alternative method to differentiate between the known mixed‐species biofilms population (Ren and others ).…”

Section: Detection Methods Of Mixed‐species Biofilmsmentioning

confidence: 99%

The Paradox of Mixed‐Species Biofilms in the Context of Food Safety

Jahid

2014

Comp Rev Food Sci Food Safe

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Formation of mixed-species biofilms constitutes a common adaptation of foodborne pathogens and indigenous microbiota for prolonged survival in their food niche. Nevertheless, the potential role of mixed-species biofilms in food safety remains to be elucidated. The formation of mixed-species biofilms on food and food processing surfaces depends on various physical, chemical, and biological processes including species composition, especially of the indigenous microbiota and nutrients, food types, temperature, quorum sensing, extracellular polymeric substance (EPS) production, biofilms maturation, and dispersal steps. Compared to monospecies, mixed-species are highly resistant to antimicrobials, possibly due to higher EPS production, internalization into food, fitness of species, denser and thicker biofilms maturation, and interspecific protection of 1 species by others, although there are much debate among studies. The fitness of mixed-species biofilms populations is suggested to be of a cooperative, competitive, or neutral nature based on the genetic background of the involved species. Currently, various methods using microarray, confocal microscopy, proteomics, and selective media are being explored for the detection of mixed-species biofilms to resolve the conflict issues. Here, we review recent progress in this emerging field in the context of food safety and propose that novel and alternative techniques like antiquorum sensing, antibiofilms, enzymes, hurdle techniques, and bacteriophages will significantly help to control the formation of mixed-species biofilms for enhanced food safety. The next challenge will be to integrate the fitness and resistance patterns of mixed-species biofilms in the laboratory with those of natural settings.

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“…Therefore, in recent years there has gradually been a shift in focus toward examining the complexity and interactions in multi-species biofilms. The use of high throughput and high resolution methods has facilitated this development in revealing complex microbial interactions including genetic, metabolite exchange and signaling to occur between microorganisms in biofilm communities ( Møller et al, 1998 ; Madsen et al, 2012 ; McLean and Kakirde, 2013 ). Such interactions may influence growth and survival of biofilm community members and also their potential virulence properties which could on their turn influence the overall pathogenicity of such structures ( Peters et al, 2012a ).…”

Section: Introductionmentioning

confidence: 99%

Intra- and inter-species interactions within biofilms of important foodborne bacterial pathogens

Giaouris

Heir

Desvaux³

et al. 2015

Front. Microbiol.

268

167

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A community-based sessile life style is the normal mode of growth and survival for many bacterial species. Under such conditions, cell-to-cell interactions are inevitable and ultimately lead to the establishment of dense, complex and highly structured biofilm populations encapsulated in a self-produced extracellular matrix and capable of coordinated and collective behavior. Remarkably, in food processing environments, a variety of different bacteria may attach to surfaces, survive, grow, and form biofilms. Salmonella enterica, Listeria monocytogenes, Escherichia coli, and Staphylococcus aureus are important bacterial pathogens commonly implicated in outbreaks of foodborne diseases, while all are known to be able to create biofilms on both abiotic and biotic surfaces. Particularly challenging is the attempt to understand the complexity of inter-bacterial interactions that can be encountered in such unwanted consortia, such as competitive and cooperative ones, together with their impact on the final outcome of these communities (e.g., maturation, physiology, antimicrobial resistance, virulence, dispersal). In this review, up-to-date data on both the intra- and inter-species interactions encountered in biofilms of these pathogens are presented. A better understanding of these interactions, both at molecular and biophysical levels, could lead to novel intervention strategies for controlling pathogenic biofilm formation in food processing environments and thus improve food safety.

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Enhancing Metagenomics Investigations of Microbial Interactions with Biofilm Technology

Cited by 17 publications

References 74 publications

Biofilms in the Food Industry: Health Aspects and Control Methods

Biofilms in the Food Industry: Health Aspects and Control Methods

The Paradox of Mixed‐Species Biofilms in the Context of Food Safety

Intra- and inter-species interactions within biofilms of important foodborne bacterial pathogens

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