Enhancing milk quality assessment with watermelon (Citrullus lanatus) urease immobilized on VS2-chitosan nanocomposite beads using response surface methodology

Kumar, Prince; Divya,; Patel, Amit Kumar; Srivastava, Anchal; Kayastha, Arvind M.

doi:10.1016/j.foodchem.2024.139447

Food Chemistry

2024

DOI: 10.1016/j.foodchem.2024.139447

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Enhancing milk quality assessment with watermelon (Citrullus lanatus) urease immobilized on VS2-chitosan nanocomposite beads using response surface methodology

Prince Kumar,

Divya,

Amit Kumar Patel

et al.

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Cited by 3 publications

References 43 publications

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Exploring the catalytic potential of watermelon urease: Purification, biochemical characterization, and heavy metal precipitation

Kumar,

Divya,

Kayastha

2024

International Journal of Biological Macromolecules

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Exploring the catalytic potential of watermelon urease: Purification, biochemical characterization, and heavy metal precipitation

Kumar,

Divya,

Kayastha

2024

International Journal of Biological Macromolecules

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Chitosan based core–shell microgel support for urease: Step up of enzyme activity, stability and storage

Chaudhary,

Pande,

Kumar

et al. 2025

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Watermelon Derived Urease Immobilized Gold Nanoparticles-Graphene Oxide Transducer for Direct Detection of Urea in Milk Samples

Kumar,

S. Dkhar,

Chandra

et al. 2024

ACS Appl. Bio Mater.

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Urea contamination in milk poses significant health risks, including kidney failure, urinary tract obstruction, fluid loss, shock, and gastrointestinal bleeding. This highlights the need for sensitive, rapid, and reliable methods to detect traces amount of urea in milk. In this study, we designed an electrochemical transducer for urea detection by utilizing purified watermelon urease (Urs), gold nanoparticles (AuNPs), and graphene oxide (GO). The nanomaterials and biosensor probe were characterized using UV−vis spectroscopy, XPS, TEM, XRD, FTIR, AFM, CV, EIS, and DPV. The engineered probe (GCE/AuNPs/GO/Urs) demonstrated a broad linear detection range of 5 to 90 mg/dL and a low limit of detection (LOD) of 0.037 (±0.012) mg/dL (RSD < 3.7%). The biosensor was tested for potential interferents that may be present in adulterated milk and an exceptionally low coefficient of selectivity (ksel <0.1) was obtained. Evaluation of milk samples from a local dairy farm showed good recovery rates from 93.13% to. 98.79% (RSD < 4.28%, n = 3), indicating reliable detection capabilities. Stability tests confirmed the sensor's reproducibility and consistent performance. Additionally, a comparison study of the system was carried out using the purified watermelon urease and the commercially available urease. Herein, the results obtained using the sensor probe was finally validated with the gold standard method.

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Enhancing milk quality assessment with watermelon (Citrullus lanatus) urease immobilized on VS2-chitosan nanocomposite beads using response surface methodology

Cited by 3 publications

References 43 publications

Exploring the catalytic potential of watermelon urease: Purification, biochemical characterization, and heavy metal precipitation

Exploring the catalytic potential of watermelon urease: Purification, biochemical characterization, and heavy metal precipitation

Chitosan based core–shell microgel support for urease: Step up of enzyme activity, stability and storage

Watermelon Derived Urease Immobilized Gold Nanoparticles-Graphene Oxide Transducer for Direct Detection of Urea in Milk Samples

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