This research aims to develop a monitoring system and temperature prediction model in neonatal premature infant incubators by applying the internet of things (IoT) concept and the 1-dimensional convolutional neural network (1D-CNN) method. The system is designed by integrating sensors, actuators, and microcontrollers connected through Wi-Fi network with message queue telemetry transport (MQTT) protocol. Sensor data in the incubator is stored in a database and displayed in real-time on a web application. The data in the database is also used for creating a temperature prediction model in the incubator. Test results indicate that the best model configuration consists of 5 neurons in the first layer, 20 neurons in the second layer, and a dense layer with 100. The evaluation of this model yields a high level of accuracy with an root mean square error (RMSE) of 0.200 °C, MSE of 0.004 °C, mean absolute error (MAE) of 0.152 °C, and mean absolute percentage error (MAPE) of 0.4%. Based on the error values obtained between the predicted and actual values from each evaluation technique in the model, it can be concluded that the range between the real and predicted values is approximately 0.2 °C. Overall, this research contributes to improving the quality of care for premature infants.