Enhancing or inhibiting apoptosis? The effects of ucMSC-Ex in the treatment of different degrees of traumatic pancreatitis

Zhao, Zhirong; Li, Han; Yiqun, He; He, Chunyang; Lichen, Zhou; Tan, Zhenghuai; Wang, Tao; Dai, Ruiwu

doi:10.1007/s10495-022-01732-1

Cited by 4 publications

(10 citation statements)

References 32 publications

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“…In addition, the unclear expression level of genes related to post‐traumatic stress and repair presents are additional challenges in understanding the development of this disease. Our previous study established a stable TP model and confirmed ucMSC‐Ex exerts reparative effects on TP 4,5,19 . In this study, we further dissected the transcriptomics after pancreatic trauma and ucMSC‐Ex therapy by high‐throughput sequencing.…”

Section: Discussionmentioning

confidence: 69%

“…In order to reduce the pancreatic tissue damage after TP and improve the follow‐up pancreatic acinar cells repair, our previous study confirmed that umbilical cord mesenchymal stem cells‐exosomes (ucMSC‐Ex) exert reparative effects on TP damaged pancreatic tissues by attenuating the inflammatory response through inhibiting the expression of inflammatory factors 4 . Furthermore, most importantly we demonstrated that ucMSC‐Ex inhibit apoptosis of pancreatic acinar cells to exert protective effects 5 . However, current research focuses on exosome mediated functional repair and tissue remodelling, and we convince that the research of exosomes should be multilevel, interdisciplinary and comprehensive 6 .…”

Section: Introductionmentioning

confidence: 76%

“…Our previous study established a stable TP model and confirmed ucMSC‐Ex exerts reparative effects on TP. 4 , 5 , 19 In this study, we further dissected the transcriptomics after pancreatic trauma and ucMSC‐Ex therapy by high‐throughput sequencing. KEGG enrichment analysis and differential analysis identified that autophagy played a crucial role in the pathophysiological process of TP and ucMSC‐Ex therapy.…”

Section: Discussionmentioning

confidence: 99%

“… 4 Furthermore, most importantly we demonstrated that ucMSC‐Ex inhibit apoptosis of pancreatic acinar cells to exert protective effects. 5 However, current research focuses on exosome mediated functional repair and tissue remodelling, and we convince that the research of exosomes should be multilevel, interdisciplinary and comprehensive. 6 Further research is required to determine the specific mechanisms by which exosomes exert therapeutic effects and the interactive effects on the regulation of cellular life activities.…”

Section: Introductionmentioning

confidence: 88%

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Still water run deep: Therapeutic TP effect of ucMSC‐Ex via regulating mTOR to enhance autophagy

Zhao,

Han,

Xin

et al. 2024

J Cellular Molecular Medi

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Our previous study confirmed that umbilical cord mesenchymal stem cells‐exosomes (ucMSC‐Ex) inhibit apoptosis of pancreatic acinar cells to exert protective effects. However, the relationship between apoptosis and autophagy in traumatic pancreatitis (TP) has rarely been reported. We dissected the transcriptomics after pancreatic trauma and ucMSC‐Ex therapy by high‐throughput sequencing. Additionally, we used rapamycin and MHY1485 to regulate mTOR. HE, inflammatory factors and pancreatic enzymatic assays were used to comprehensively determine the local versus systemic injury level, fluorescence staining and electron microscopy were used to detect the effect of autophagy, and observe the expression levels of autophagy‐related markers at the gene and protein levels. High‐throughput sequencing identified that autophagy played a crucial role in the pathophysiological process of TP and ucMSC‐Ex therapy. The results of electron microscopy, immunofluorescence staining, polymerase chain reaction and western blot suggested that therapeutic effect of ucMSC‐Ex was mediated by activation of autophagy in pancreatic acinar cells through inhibition of mTOR. ucMSC‐Ex can attenuate pancreas injury by inhibiting mTOR to regulate acinar cell autophagy after TP. Future studies will build on the comprehensive sequencing of RNA carried by ucMSC‐Ex to predict and verify specific non‐coding RNA.

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Section: Discussionmentioning

confidence: 69%

Section: Introductionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 88%

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Still water run deep: Therapeutic TP effect of ucMSC‐Ex via regulating mTOR to enhance autophagy

Zhao,

Han,

Xin

et al. 2024

J Cellular Molecular Medi

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“…Three studies utilized MSCs from allogeneic mice/rats [ 33 , 36 , 37 ], while others introduced human MSCs [ 32 , 34 , 35 ]. The types of MSC used were dispersed from umbilical cord- derived mesenchymal stem cells (UC-MSCs) [ 32 ], hair follicle-derived mesenchymal stem cells (HF-MSCs) [ 33 ], adipose-derived mesenchymal stem cells (AD-MSCs) [ 35 , 36 ], and bone marrow mesenchymal stem cells (BM-MSCs) [ 37 ], to induced stem cells (iMSCs) [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

Prospect of Mesenchymal Stem-Cell-Conditioned Medium in the Treatment of Acute Pancreatitis: A Systematic Review

Pang,

Kong,

2023

Biomedicines

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Mesenchymal stem cells (MSCs) have demonstrated potential in both clinical and pre-clinical research for mitigating tissue damage and inflammation associated with acute pancreatitis (AP) via paracrine mechanisms. Hence, there has been a recent surge of interest among researchers in utilizing MSC cultured medium (CM) and its components for the treatment of AP, which is recognized as the primary cause of hospitalization for gastrointestinal disorders globally. A systematic review was conducted by searching the MEDLINE, EMBASE, and Web of Science databases. Studies that involve the administration of MSC-CM, extracellular vesicles/microvesicles (EVs/MVs), or exosomes to AP animal models are included. A total of six research studies, including eight experiments, were identified as relevant. The findings of this study provide evidence in favor of a beneficial impact of MSC-CM on both clinical and immunological outcomes. Nevertheless, prior to clinical trials, large animal models should be used and prolonged observation periods conducted in pre-clinical research. Challenges arise due to the lack of standardization and consensus on isolation processes, quantifications, and purity testing, making it difficult to compare reports and conduct meta-analyses in MSC-CM-based therapies.

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The dual role of mesenchymal stem cells in apoptosis regulation

Chen,

Xia,

Yao

et al. 2024

Cell Death Dis

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Mesenchymal stem cells (MSCs) are widely distributed pluripotent stem cells with powerful immunomodulatory capacity. MSCs transplantation therapy (MSCT) is widely used in the fields of tissue regeneration and repair, and treatment of inflammatory diseases. Apoptosis is an important way for tissues to maintain cell renewal, but it also plays an important role in various diseases. And many studies have shown that MSCs improves the diseases by regulating cell apoptosis. The regulation of MSCs on apoptosis is double-sided. On the one hand, MSCs significantly inhibit the apoptosis of diseased cells. On the other hand, MSCs also promote the apoptosis of tumor cells and excessive immune cells. Furthermore, MSCs regulate apoptosis through multiple molecules and pathways, including three classical apoptotic signaling pathways and other pathways. In this review, we summarize the current evidence on the regulation of apoptosis by MSCs.

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Enhancing or inhibiting apoptosis? The effects of ucMSC-Ex in the treatment of different degrees of traumatic pancreatitis

Cited by 4 publications

References 32 publications

Still water run deep: Therapeutic TP effect of ucMSC‐Ex via regulating mTOR to enhance autophagy

Still water run deep: Therapeutic TP effect of ucMSC‐Ex via regulating mTOR to enhance autophagy

Prospect of Mesenchymal Stem-Cell-Conditioned Medium in the Treatment of Acute Pancreatitis: A Systematic Review

The dual role of mesenchymal stem cells in apoptosis regulation

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