Enhancing Oriented Immobilization Efficiency: A One-for-Two Organism-Bispecific Nanobody Scaffold for Highly Sensitive Detection of Foodborne Pathogens
Yueqi Wang,
Liang Zhang,
Peng Wang
et al.
Abstract:Nanobodies
have gained widespread application in immunoassays.
However, their small size presents a significant challenge in achieving
effective immobilization and optimal sensitivity. Here, we present
a novel “one-for-two”-oriented immobilization platform
based on an organism-bispecific nanobody (O-BsNb) scaffold, enabling
highly sensitive detection of two bacterial pathogens. Through genetic
engineering, a bispecific nanobody (BsNb) was engineered, targeting Salmonella spp. and Vibrio parahaemolyticus. The O… Show more
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