Enhancing oxygen solubility using hemoglobin- and perfluorocarbon-based carriers

Centis,; Vermette, Patrick

doi:10.2741/3272

Cited by 21 publications

(24 citation statements)

References 99 publications

(131 reference statements)

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“…For instance, artificial oxygen carriers, like perfluorocarbon or modified hemoglobin, have been used to increase oxygen transport and transfer within biomaterials [25]. However, their use is associated with systemic side effects such as vasoconstriction [26][27][28] and the toxic build-up of compounds due to failure of metabolic removal [28].…”

Section: Discussionmentioning

confidence: 99%

Photosynthetic biomaterials: A pathway towards autotrophic tissue engineering

et al. 2015

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Section: Discussionmentioning

confidence: 99%

Photosynthetic biomaterials: A pathway towards autotrophic tissue engineering

et al. 2015

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“…7,8 The use of O 2 carriers in the cell culture system, on the other hand, provides a biomimetic approach to recapitulate the in vivo oxygenation environment for many tissue engineering applications. [9][10][11] In particular, hepatic hollow fiber (HF) bioreactors that constitute one type of bioartificial liver assist device (BLAD) suffer from O 2 limited transport mainly due to the low solubility of O 2 in the cell culture medium, long diffusion pathlengths, and high demand for O 2 by the hepatocytes cultured in the extracapillary space (ECS). [12][13][14] These devices are expected to bridge patients suffering from acute liver failure toward native liver regeneration or orthotopic liver transplantation by providing sufficient global liver functions.…”

Section: Introductionmentioning

confidence: 99%

Hemoglobin Regulates the Metabolic, Synthetic, Detoxification, and Biotransformation Functions of Hepatoma Cells Cultured in a Hollow Fiber Bioreactor

Chen

Palmer

2010

Tissue Engineering Part A

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Hepatic hollow fiber (HF) bioreactors constitute one type of extracorporeal bioartificial liver assist device (BLAD). Ideally, cultured hepatocytes in a BLAD should closely mimic the in vivo oxygenation environment of the liver sinusoid to yield a device with optimal performance. However, most BLADs, including hepatic HF bioreactors, suffer from O 2 limited transport toward cultured hepatocytes, which reduces their performance. We hypothesize that supplementation of hemoglobin-based O 2 carriers into the circulating cell culture medium of hepatic HF bioreactors is a feasible and effective strategy to improve bioreactor oxygenation and performance. We examined the effect of bovine hemoglobin (BvHb) supplementation (15 g/L) in the circulating cell culture medium of hepatic HF bioreactors on hepatocyte proliferation, metabolism, and varied liver functions, including biosynthesis, detoxification, and biotransformation. It was observed that BvHb supplementation supported the maintenance of a higher cell mass in the extracapillary space, improved hepatocyte metabolic efficiency (i.e., hepatocytes consumed much less glucose), improved hepatocyte capacity for drug metabolism, and conserved both albumin synthesis and ammonia detoxification functions compared to controls (no BvHb supplementation) under the same experimental conditions.

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“…Based on equimolar intracellular biochemical reactions of monosaccharides oxidation, oxygen is typically consumed at approximately the same rate as glucose, but oxygen solubility is much lower than the availability of glucose in a typical culture medium (i.e., ca. 20 -200 mM depending on type of cultured cells) (Centis and Vermette, 2009;Martin and Vermette, 2005).…”

Section: Introductionmentioning

confidence: 99%

Liquid Perfluorochemicals as Flexible and Efficient Gas Carriers Applied in Bioprocess Engineering: An Updated Overview and Future Prospects

Pilarek¹

2014

Chemical and Process Engineering

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Fully synthetic, biochemically inert and water-immiscible liquid perfluorochemicals (PFCs) are recognised as flexible liquid carriers/scavengers of gaseous compounds (respiratory gases mainly, i.e. O 2 and CO 2 ) and increasingly applied in bioprocess engineering. A range of unmatched physicochemical properties of liquid PFCs, i.e. outstanding chemo-and thermostability, extremely low surface tension, simultaneous hydro-and lipophobicity, which result from carbon chain substitution with fluorine atoms (the most electronegative chemical element) and the presence of intramolecular C-F bonds (the strongest single bond known in organic chemistry) have been described in detail. Exceptional propensity to solubility of respiratory gases in liquid perfluorinated compounds has been widely discussed. Advantages and disadvantages of bioprocess applications of liquid PFCs in the form of a pure PFC as well as in an emulsified form have been pointed out. A liquid PFC-mediated mass transfer intensification in various types of microbial, plant cell and animal cell culture systems: from miniaturised microlitre-scale cultures, via biomaterial-based scaffolds containing culture systems, to litre-scale bioreactors, has been reviewed and elaborated on bearing in mind the benefits of bioprocesses.

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Enhancing oxygen solubility using hemoglobin- and perfluorocarbon-based carriers

Cited by 21 publications

References 99 publications

Photosynthetic biomaterials: A pathway towards autotrophic tissue engineering

Photosynthetic biomaterials: A pathway towards autotrophic tissue engineering

Hemoglobin Regulates the Metabolic, Synthetic, Detoxification, and Biotransformation Functions of Hepatoma Cells Cultured in a Hollow Fiber Bioreactor

Liquid Perfluorochemicals as Flexible and Efficient Gas Carriers Applied in Bioprocess Engineering: An Updated Overview and Future Prospects

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