Enhancing Peptide Biomaterials for Biofabrication

Abstract

Biofabrication using well-matched cell/materials systems provides unprecedented opportunities for dealing with human health issues where disease or injury overtake the body’s native regenerative abilities. Such opportunities can be enhanced through the development of biomaterials with cues that appropriately influence embedded cells into forming functional tissues and organs. In this context, biomaterials’ reliance on rigid biofabrication techniques needs to support the incorporation of a hierarchical mimicry of local and bulk biological cues that mimic the key functional components of native extracellular matrix. Advances in synthetic self-assembling peptide biomaterials promise to produce reproducible mimics of tissue-specific structures and may go some way in overcoming batch inconsistency issues of naturally sourced materials. Recent work in this area has demonstrated biofabrication with self-assembling peptide biomaterials with unique biofabrication technologies to support structural fidelity upon 3D patterning. The use of synthetic self-assembling peptide biomaterials is a growing field that has demonstrated applicability in dermal, intestinal, muscle, cancer and stem cell tissue engineering.

Short Peptide Nanofiber Biomaterials Ameliorate Local Hemostatic Capacity of Surgical Materials and Intraoperative Hemostatic Applications in Clinics

“…[ 10 ] Designer self‐assembling peptide (dSAP) nanomaterials intend to produce biomimics of tissue‐specific nanostructures and support the inherent incorporation of local and bulk biological cues via hierarchical mimicry. [ 11 ] This fabrication technology at the atomic scale or molecular level mainly focuses on designing various peptide nanofiber biomaterial devices, [ 12 ] which is becoming an important trend in biomedical nanomaterial strategy and aims to resolve current limitations of hemostatic biomaterial research in surgical use, especially in surgical procedures on tissues that are difficult to access, impractical to mechanical and thermal treatments, or too delicate to conduct suturing. From a biochemical perspective, these dSAP building blocks are remarkably innovative nanomaterials with notable biocompatibility and defined tissue‐sealing efficiency in NCTH and IMB scenarios.…”

Rational design of potent ultrashort antimicrobial peptides with programmable assembly into nanostructured hydrogels

“…Such supramolecular hydrogels can be exploited to create biomaterials with various applications, including as drug delivery vehicles, tissue engineering scaffolds, or as precursors for creating nanostructured surfaces (Das and Gavel, 2020;Cardoso et al, 2021;Shi et al, 2021). Specifically, the use of a fluorenylmethyloxycarbonyl (Fmoc) group located at the N-terminal of the sequence was previously reported to drive peptide supramolecular assembly into biocompatible hydrogels (Fleming et al, 2013;Tao et al, 2016;Firipis et al, 2021).…”

Peptide–Protein Coassemblies into Hierarchical and Bioactive Tubular Membranes

“…14 However, these systems alone remain far from recreating the diverse multiscale signaling observed in the native extracellular matrix (ECM). 15 Multicomponent self-assembly faciliates controlled integration of multiple types of building-blocks leading to materials with properties that not only combine those of the individual components but also emergent ones. 16 Through this approach, O'Reilly and co-workers developed a temperature-responsive bioconjugate system of superfolder green fluorescent protein (sfGFP) and poly[(oligo ethylene glycol) methyl ether methacrylate] (PEGMA).…”

“…The versatility of these molecules also enables structural modifications by incorporating matrix metalloproteinase cleavage sites in order to reveal a hidden bioactive region after controlled degradation, integrating host–guest moieties to tailor interfiber interactions, − or enabling hierarchical assembly into aligned nanofibers . However, these systems alone remain far from recreating the diverse multiscale signaling observed in the native extracellular matrix (ECM) …”