Enhancing sealing of fetal membrane defects using tissue engineered native amniotic scaffolds in the rabbit model

Fetoscopic Closure of Punctured Fetal Membranes With Acellular Human Amnion Plugs in a Rabbit Model

“…These experimental group sizes compared those used by us others in this rabbit model. 8,11,16 A 2-mm to 3-mm myometrial incision was created with microscissors as already described 7,16 to separate the chorion and allow the amniotic membrane to bulge through the chorionic incision after gentle uterine pressure (Fig. 3).…”

“…10 We tested acellular human amnion plugs in the midgestational rabbit model that constitutes a well-accepted, very challenging model with a loose relationship between fetal membranes and the uterine wall. As stated by Ochsenbein-Kolble et al, 11 although in rabbits the relationship of the fetal membranes to the decidua and myometrium is much different from that in humans or nonhuman primates, surgically induced membrane defects that are left uncovered will persist in most cases, leading to oligohydramnios and its consequences.…”

“…The sizes of experimental groups in the present study compared those used by us and others in this rabbit model. 8,11,16 For ethical as well as cost considerations, we kept number of animals in our initial exploration of decellularized term human amnion membrane plugs minimal. Although both treatments with decellularized term human amnion membrane and commercial collagen matrix foil appeared to be working treatments, we could not substantiate our observations by statistics.…”

Injectable candidate sealants for fetal membrane repair: bonding and toxicity in vitro

“…Of note, collagen fleece plugs (Lyostypt) are now routinely used for prophylactic plugging of iatrogenic membrane defects following fetoscopic endoluminal tracheal occlusion for in utero therapy of congenital diaphragmatic hernia in one center of the authors, in Leuven. Other prophylactic plugging techniques such as scaffold-type plugs manufactured directly from decellularized amnion tissue have been so far only evaluated in animal models 9,10. Further, also laser welding, pre-emptive placement of synthetic surgical sealants before fetoscopic access, direct injection into amnotic fluid of fibrinogen/thrombin-based tissue sealant, and sealing with platelet-rich plasma were evaluated in laboratory settings 11,12,13,14.…”

Traitement de la rupture iatrogène des membranes fœtales par « patch » amniotique

“…Nous avons dé montré que cela augmentait le nombre de cellules prolifé ratives (venant en partie du liquide amniotique) dans le plug [19,20]. Nous sommes toujours à la recherche de matrices plus efficaces, comme des matrices dé rivé es de membranes foetales, qui pourraient être ensemencé es de cellules pour en amé liorer la croissance [21,22].…”