Enhancing secondary metabolite production in plants: Exploring traditional and modern strategies

Selwal, Nidhi; Rahayu, Farida; Herwati, Anik; Latifah, Evy; Supriyono, Supriyono; Suhara, Cece; Suastika, Ida Bagus Kade; Mahayu, Weda Makarti; Wani, Atif Khurshid

doi:10.1016/j.jafr.2023.100702

Cited by 16 publications

(4 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results obtained for the optimization of the media for AgNPs proved that differences in the medium components affect the production of secondary metabolites and ultimately influence nanoparticle production using the isolates. Similar reports on MGYP for the biosynthesis of various NPs have been found [ 62 ]. So, there is a need to optimize the medium components using statistical design [ 63 ].…”

Section: Discussionsupporting

confidence: 86%

Exploring the Potential of Halotolerant Actinomycetes from Rann of Kutch, India: A Study on the Synthesis, Characterization, and Biomedical Applications of Silver Nanoparticles

Dayma,

Choudhary,

Ali

et al. 2024

Pharmaceuticals

View full text Add to dashboard Cite

A tremendous increase in the green synthesis of metallic nanoparticles has been noticed in the last decades, which is due to their unique properties at the nano dimension. The present research work deals with synthesis mediated by the actinomycete Streptomyces tendae of silver nanoparticles (AgNPs), isolated from Little and Greater Rann of Kutch, India. The confirmation of the formation of AgNPs by the actinomycetes was carried out by using a UV-Vis spectrophotometer where an absorbance peak was obtained at 420 nm. The X-ray diffraction pattern demonstrated five characteristic diffraction peaks indexed at the lattice plane (111), (200), (231), (222), and (220). Fourier transform infrared showed typical bands at 531 to 1635, 2111, and 3328 cm−1. Scanning electron microscopy shows that the spherical-shaped AgNPs particles have diameters in the range of 40 to 90 nm. The particle size analysis displayed the mean particle size of AgNPs in aqueous medium, which was about 55 nm (±27 nm), bearing a negative charge on their surfaces. The potential of the S. tendae-mediated synthesized AgNPs was evaluated for their antimicrobial, anti-methicillin-resistant Staphylococcus aureus (MRSA), anti-biofilm, and anti-oxidant activity. The maximum inhibitory effect was observed against Pseudomonas aeruginosa at (8 µg/mL), followed by Escherichia coli and Aspergillus niger at (32 µg/mL), and against Candida albicans (64 µg/mL), whereas Bacillus subtilis (128 µg/mL) and Staphylococcus aureus (256 µg/mL) were much less sensitive to AgNPs. The biosynthesized AgNPs displayed activity against MRSA, and the free radical scavenging activity was observed with an increase in the dosage of AgNPs from 25 to 200 µg/mL. AgNPs in combination with ampicillin displayed inhibition of the development of biofilm in Pseudomonas aeruginosa and Streptococcus pneumoniae at 98% and 83%, respectively. AgNPs were also successfully coated on the surface of cotton to prepare antimicrobial surgical cotton, which demonstrated inhibitory action against Bacillus subtilis (15 mm) and Escherichia coli (12 mm). The present research integrates microbiology, nanotechnology, and biomedical science to formulate environmentally friendly antimicrobial materials using halotolerant actinomycetes, evolving green nanotechnology in the biomedical field. Moreover, this study broadens the understanding of halotolerant actinomycetes and their potential and opens possibilities for formulating new antimicrobial products and therapies.

show abstract

Section: Discussionsupporting

confidence: 86%

Exploring the Potential of Halotolerant Actinomycetes from Rann of Kutch, India: A Study on the Synthesis, Characterization, and Biomedical Applications of Silver Nanoparticles

Dayma,

Choudhary,

Ali

et al. 2024

Pharmaceuticals

View full text Add to dashboard Cite

show abstract

“…Plant cell technology has been explored for the production of natural or recombinant compounds of commercial interest and has received increasing consideration currently as well as in the past decades. It is a potential tool for triggering the plant metabolism for the production of valuable bioactive compounds (Selwal et al, 2023). Cell suspension culture is more preferable than any other techniques for the large scale production of secondary metabolites naturally due to its rapid growth cycles.…”

Section: Discussionmentioning

confidence: 99%

Enhanced production of ‘spilanthol’ through elicitation of cell suspension cultures in Acmella ciliata (Kunth) Cass. and spilanthol characterization by HPLC-HRMS analysis

NeethuMohan,

Hemanthakumar,

Preetha

2024

Preprint

View full text Add to dashboard Cite

The study presented here has established a cell suspension culture system for the in vitro production of the high value bioactive alkamide ‘spilanthol’ in Acmella ciliata. White, purple, friable callus procured from leaf explants in MS medium containing 0.5 mgl-1 BA and 1.0 mgl-1 NAA have succeeded in fourfold increase in cell mass after 25 days of culture upon 120 rpm agitation in a gyratory shaker and the presence of ‘spilanthol’ in the harvested cells was detected using HPLC coupled with HRMS. Here, high spilanthol content (239.512 µgg-1) was noticed in embryogenic callus cultures established in NAA-BA combination followed by in vitro plant (193.935 µgg-1) as well as cells harvested from suspension culture (173.702 µgg-1). In A. ciliata flower heads 102.730 µgg-1 spilanthol content was recorded, while the leaves and stem contained 96.69 and 61.146 µgg-1 spilanthol respectively. The least quantity of spilanthol was noticed in the in vivo plant (92.198 µgg-1) that was the absolute control. Thus quantification of spilanthol in the in vitro system revealed more amount of the same in it especially in embryogenic callus than in vivo plant (control). Elicitation was attempted using both biotic and abiotic elicitors to scale up the production of spilanthol in cell suspension culture system. Elicitation using biotic elicitors showed maximum 34.61-fold increase with Yeast extract (YE) in 24 hrs exposure; while the abiotic elicitor MeJA (Methyl jasmonate) treatment evoked the production of spilanthol to 41.02 fold in 72 hrs treatment. Comparatively more time of exposure was required in abiotic elicitation than with biotic elicitors. By considering the merit of perceiving maximum yield in short duration, treatment with YE for 24 hrs period can be suggested as the choice of elicitation for improved production of the alkamides particularly spilanthol in cell suspension cultures of A. ciliata. The established system can be extended for the bioproduction of the bioactive alkamide ‘spilanthol’ using bioreactor technology with suitable refinement thus benefiting the phyto-pharma needs.

show abstract

“…However, the yield of secondary metabolites obtained from natural populations of plants is insufficient to meet commercial demands due to their limited accumulation [81]. Various in vitro culture techniques, including callus, hairy root, shoot, and suspension cultures, can be utilized to enhance the biosynthesis of specific metabolites in commercially important plants [82]. Among these techniques, callus culture is regarded as a promising approach for the biosynthesis of bioactive compounds in endangered species of most medicinal plants [83].…”

Section: Discussionmentioning

confidence: 99%

Effect of Different Culture Conditions on Anthocyanins and Related Genes in Red Pear Callus

Yao,

Lei,

Zhou

et al. 2023

Agronomy

View full text Add to dashboard Cite

Red pears are appreciated for their abundant nutritional benefits and visually striking red hue, rendering them a favored option among consumers and stimulating substantial market demand. The present study employs the flesh of a red pear as the explant, subjecting the flesh callus to varying sugar sources, MS concentrations, light qualities, and temperatures to investigate the alterations in secondary metabolites, including anthocyanins, within the callus. It was found that sucrose can induce more anthocyanins, and its related metabolites and genes also increase as the sucrose and MS concentrations increase. Under the conditions of red-blue light and a temperature of 15 °C, it can further induce the production of more anthocyanins and secondary metabolites and can also upregulate the synthesis of anthocyanin-related genes. As such, this investigation serves to elucidate the factors that contribute to anthocyanin accumulation in red pears, thereby providing a theoretical foundation for understanding the mechanisms underlying color change.

show abstract

Enhancing secondary metabolite production in plants: Exploring traditional and modern strategies

Cited by 16 publications

References 72 publications

Exploring the Potential of Halotolerant Actinomycetes from Rann of Kutch, India: A Study on the Synthesis, Characterization, and Biomedical Applications of Silver Nanoparticles

Exploring the Potential of Halotolerant Actinomycetes from Rann of Kutch, India: A Study on the Synthesis, Characterization, and Biomedical Applications of Silver Nanoparticles

Enhanced production of ‘spilanthol’ through elicitation of cell suspension cultures in Acmella ciliata (Kunth) Cass. and spilanthol characterization by HPLC-HRMS analysis

Effect of Different Culture Conditions on Anthocyanins and Related Genes in Red Pear Callus

Contact Info

Product

Resources

About