Enhancing Skin Cancer Diagnosis with Dermoscopy

Wolner, Zachary; Yélamos, Oriol; Liopyris, Konstantinos; Rogers, Tova; Marchetti, Michael A.; Marghoob, Ashfaq A.

doi:10.1016/j.det.2017.06.003

Cited by 87 publications

(76 citation statements)

References 76 publications

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“…Early detection: Detection of melanoma by self-examination is common and very important, clinical recognition, though, allows for the earlier detection of melanoma and improved clinical management (Carli et al, 2004, Wolner et al, 2017. The "ugly duckling" sign (Grob & Bonerandi, 1998)…”

Section: Signs and Symptomsmentioning

confidence: 99%

Identification and clinical evaluation of senescence-associated markers to distinguish melanocytic nevi from melanomas

Orfanidis

2020

Linköping University Medical Dissertations

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Melanoma is a form of cancer that develops in melanocytes. While it represents only 5% of skin malignancies, it is the most aggressive and lethal. Benign proliferation of these cells form the melanocytic nevi. The definitive diagnosis of melanocytic nevi or melanoma lesions is histopathologic. However, it is estimated that a correct diagnosis is established by means of standard skin biopsy in only 83% of the melanocytic lesions; of the remaining cases 8% and 9% are overinterpreted (false positives) and under-interpreted (false negatives), respectively. This underscores the importance of additional diagnostic tests. Since cellular senescence is considered to be a tumor suppressive mechanism, immuno-histochemistry using senescence markers has been suggested for the evaluation of difficult melanocytic lesions; however, the routinely used senescence markers lack the ability to distinguish nevi from melanoma. The general aim of this thesis is therefore to identify novel senescence markers that may aid in melanoma diagnosis.In study I, we established a cellular model with nevus-mimicking characteristics consisting in primary melanocytes that become senescent. Transcriptomic analysis allowed expanding the set of senescence-associated markers that could distinguish nevi from melanoma and identifying tubulin β-3 as a potential diagnostic marker. Depletion of tubulin β-3 and pretreatment with tubulin destabilizing drugs in melanocytes and melanoma cells induced a senescence-like phenotype in vitro. In particular, reduced migration capacity and induction of cell cycle arrest in G2/M phase of the cell cycle was demonstrated.In study II, a potential inter-cellular signaling pathway between melanoma cells and stromal fibroblasts, that might facilitate melanoma invasion, was investigated. Ultraviolet (UV) radiation was shown, both in melanoma cells and fibroblasts, to promote the release and activation of TGF-β1 and subsequent increase in expression of the serine protease FAP-α, a protein that plays role in extracellular matrix degradation and therefore facilitates the invasion of melanoma cells. Such mechanism was not functional in senescent melanocytes.In study III, it was shown that tubulin β-3 immunostaining aids in the diagnosis of nevi and melanomas. The diagnostic criterium was the tubulin β-3 gradient within the melanocytic nevi that was no longer apparent in melanoma. Different patterns of tubulin β-3 immunostaining in melanoma were described, dermoscopy-immunohistochemistry associations were found, specific dermoscopic features highlighted, and the prognostic value of this tubulin β-3 marker was examined. The progression rate in patients whose melanomas had areas with loss of tubulin β-3 was 4 times higher than in patients without this feature, although statistical significance could not be reached (p=0.06).In conclusion, transcriptomic analysis expanded the set of senescence-associated markers that could distinguish nevi from melanoma and identified tubulin β-3 as novel immunohistochemistry marker shown to have diagn...

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Section: Signs and Symptomsmentioning

confidence: 99%

Identification and clinical evaluation of senescence-associated markers to distinguish melanocytic nevi from melanomas

Orfanidis

2020

Linköping University Medical Dissertations

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“…Hátránya, hogy a dermatoszkópos vizsgálat során észlelhető jellegzetességek, gyakran nem specifikusak, így eltérő eredetű elváltozások is mutathatnak hasonló képet, így a diagnosztikus pontosság nagyban függ a vizsgáló jártasságától. Emellett a dermatoszkópia nem alkalmas a tumorok mélységbeli kiterjedésének meghatározására, amely melanoma esetén a legfontosabb prognosztikus faktor és elengedhetetlen a terápiás terv kialakításához (15).…”

Section: Táblázatunclassified

Novel imaging techniques and their clinical applications in the field of dermatology

Kiss¹,

Dermatooncology²,

Anker³

et al. 2019

BVSZ

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ÖSSZEFOGLALÁSAz elmúlt évtizedekben az orvosi képalkotás fejlődése jelentős előrelépéseket hozott a bőrgyógyászatban. Bár jelenleg a hisztopatológiai vizsgálat az "arany standard" diagnosztikus módszer, a már-már szövettani felbontást lehetővé tévő in vivo, non-invazív képalkotási módszerek számos bőrgyógyászati kórkép vizsgálatára hatékonyan alkalmazhatók. A szerzők összefoglalják a bőrgyógyászat területén már széleskörűen alkalmazott, valamint az ígéretes, de egyelőre kísérleti stádiumban lévő bőrgyógyászati képalkotó módszereket. Az egyes modalitások elméleti hátterének ismertetése mellett részletezésre kerülnek a különböző technikák klinikai alkalmazásának lehetőségei. Kulcsszavak: bőrgyógyászati képalkotásoptikai koherencia tomográfia -reflektancia konfokális mikroszkópianemlineáris mikroszkópia SUMMARYThe development of novel medical imaging tools has resulted in significant progression in the field of dermatology. However, histopathological evaluation still remains the gold standard diagnostic method. Novel, in vivo non-invasive imaging modalities can provide near histological resolution and can be applied in the assessment of various dermatological diseases. In this paper, the authors review the clinically applied imaging techniques among the newest experimental devices. After the description of the basic principles and imaging properties of these techniques, the authors also discuss their clinical applications.

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“…To the Editor: Dermoscopy improves diagnostic accuracy and reduces biopsy rates. 1 In resourcelimited settings, where anatomic pathology services are scarce, dermoscopy has the potential to enhance quality of care with a relatively modest capital investment. Further, there are emerging uses for dermoscopy in pediatric dermatology.…”

Section: Teaching Pediatric Dermoscopy In a Resource-limited Settingmentioning

confidence: 99%

Teaching pediatric dermoscopy in a resource-limited setting

Gurnee

Obeng

Stoff

2018

Journal of the American Academy of Dermatology

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Enhancing Skin Cancer Diagnosis with Dermoscopy

Cited by 87 publications

References 76 publications

Identification and clinical evaluation of senescence-associated markers to distinguish melanocytic nevi from melanomas

Identification and clinical evaluation of senescence-associated markers to distinguish melanocytic nevi from melanomas

Novel imaging techniques and their clinical applications in the field of dermatology

Teaching pediatric dermoscopy in a resource-limited setting

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