Enhancing Specific Binding of L929 Fibroblasts: Effects of Multi‐Scale Topography of GRGDY Peptide Modified Surfaces

Abstract

Surface chemical composition and roughness caused by micro/nano-topographical structures are two predominant factors in determining cellular response. A series of gold nanoparticle layers (GNPLs) with increasing surface roughness are prepared by depositing gold nanoparticles onto planar gold films. Glycine-arginine-glycine-aspartic acid-tyrosine (GRGDY) peptide is then conjugated to the surface through poly[oligo(ethylene glycol) methacrylate] polymer brushes as spacers. The pristine micro/nano-roughness structures significantly inhibit L929 cell growth. After modification with POEGMA-GRGDY, however, the micro/nano-structures greatly enhance L929 cell-specific interactions while maintaining superior low-fouling ability compared to planar gold.

Maintaining the pluripotency of mouse embryonic stem cells on gold nanoparticle layers with nanoscale but not microscale surface roughness

“…Previous studies have already demonstrated that gold nanoparticle layers (GNPLs) can be easily prepared using a chemical gold plating method, and their surface roughnesses can be regulated by adjusting the mole number of hydrogen tetrachloroaurate acid (from 1.8 mmol to 6.0 mmol) in the gold plating solution. 27,32,33 To investigate the biological effects of the GNPL surface on a larger surface roughness scale in this study, we increased the mole number of hydrogen tetrachloroaurate acid to 9.6 mmol in the gold plating solution. Five types of GNPL-modied Au surfaces were fabricated and termed GL-1, GL-2, GL-3, GL-4 and GL-5 in the order of increasing hydrogen tetrachloroaurate mole number.…”

“…The aminated surfaces were then rinsed with ethanol (at least 3 times) and dried in a stream of nitrogen. The GNPLs were prepared as described by Zhou et al 27 Briey, the aminated surfaces were placed in the wells of a 48-well plate; then, 80, 160, 300, 500 or 800 mL plating solutions (12 mM HAuCl 4 $4H 2 O, 0.5 M KHCO 3 , and 25 mM glucose) containing 0.96, 1.92, 3.60, 6.00 or 9.60 mmol hydrogen tetrachloroaurate acid, respectively, were added to each well and incubated at 37 C for 3 h to form gold nanoparticle layers. The slides were removed from the solution, rinsed with deionized water, and dried in a stream of nitrogen.…”

Direct micrometer patterning and functionalization of polymer blend surfaces by using hot embossing

“…Among others, two main factors have been identified to play a crucial role in the final surface properties and therefore have to be included in the design of a material for a targeted application: surface morphology and chemical composition [47][48][49][50][51]. Herein we propose an alternative to multistep patterning and functionalization procedures to vary both properties in a straightforward manner.…”

Preventing infection of osseointegrated transcutaneous implants: Incorporation of silver into preconditioned fibronectin-functionalized hydroxyapatite coatings suppresses Staphylococcus aureus colonization while promoting viable fibroblast growth in vitro

“…38,39 Fibroblasts are known to be sensitive to surface topography and previous studies have shown that reductions in roughness even on a nanometric scale are associated with improved fibroblast attachment, proliferation, or viability. [40][41][42][43][44][45] However, many factors such as wettability and surface chemistry influence fibroblast responses and may change the way fibroblasts to respond to surfaces. This may enable cells to grow on rougher surfaces despite the fact that they usually respond better to smoother surfaces.…”