Enhancing stability and activity of β-galactosidase from Kluyveromyces lactis through immobilization on polymethacrylate monolith and optimisation using response surface methodology
Dahari, R. F.,
Misson, M.,
Ongkudon, C. M.
et al.
Abstract:Aims: This study investigates the potential of porous polymethacrylate monoliths as enzyme support materials for largescale enzyme commercialization. Methodology and results: It focuses on their preparation and various immobilization techniques, such as adsorption, covalent-binding and cross-linking, specifically applied to β-galactosidase for bioprocess applications. The research assesses immobilization performance, operational stability, reusability and optimization using response surface methodology (RSM). … Show more
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