2024
DOI: 10.1021/cbe.4c00012
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Enhancing Substrate Preference of Iridoid Synthase via Focused Polarity-Steric Mutagenesis Scanning

Huifen Yu,
Cuifang Ye,
Yong Wang
et al.

Abstract: Nepetalactol serves as the scaffold for most iridoids, which exhibit a wide range of biological and pharmacological activities. Iridoid synthase (ISY) plays a crucial role in the in vivo synthesis of nepetalactol from 8-oxogeranial. However, the substrate promiscuity of ISY could result in a deviation of flux toward off-target routes. In this work, the substrate preference (SP, the ratio of activity for 8-oxogeranial to geranial) of ISY for nepetalactol was improved by directed evolution. First, the strategy o… Show more

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