Enhancing the AI-2/LuxS quorum sensing system in Lactiplantibacillus plantarum: Effect on the elimination of biofilms grown on seafoods

Qian, Yilin; Zhao, Chao; Cai, Xinya; Zeng, Mingyong; Liu, Zunying

doi:10.1016/j.ijfoodmicro.2023.110102

Cited by 6 publications

(4 citation statements)

References 48 publications

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“…Studies on the positive and negative regulation of strain QS have shown that QS, various physiological and metabolic activities of strains, can be regulated by the addition of exogenous substances 24–26 . However, there are fewer existing studies on the positive regulation of strain QS.…”

Section: Introductionmentioning

confidence: 99%

“…However, there are fewer existing studies on the positive regulation of strain QS. Currently, positive regulation is mainly achieved through the exogenous addition of signal molecules or the overexpression of related genes 24,27 . However, fewer studies are using the organic selenium to positively and negatively regulate the QS of strains, and studies on the regulatory mechanism have not been reported.…”

Section: Introductionmentioning

confidence: 99%

“…23 Studies on the positive and negative regulation of strain QS have shown that QS, various physiological and metabolic activities of strains, can be regulated by the addition of exogenous substances. [24][25][26] However, there are fewer existing studies on the positive regulation of strain QS. Currently, positive regulation is mainly achieved through the exogenous addition of signal molecules or the overexpression of related genes.…”

mentioning

confidence: 99%

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Regulation and mechanism of organic selenium on quorum sensing, biofilm, and antioxidant effects of Lactobacillus paracasei

Lou,

Dong,

Tao

et al. 2024

Cell Biochemistry & Function

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Different organic compounds can have varying degrees of impact on the activity of Lactobacillus paracasei. The study focused on the impact and action mechanism of different organic selenium products on the bioactivity of two strains of L. paracasei. The growth, antioxidant activity, extracellular polysaccharide secretion, quorum sensing (QS), and biofilm formation of the strains before and after the addition of organic selenium crude products and three organic selenium standard were evaluated. The results showed that the addition of crude organic selenium promoted the various activities of the strain. l‐selenocysteine had the strongest regulatory effect, with maximum GIM1.80 biofilm formation when it reached a critical concentration of 0.4 μg/mL; l‐selenomethionine resulted in the highest activity of the signal molecule Auto inducer‐2 of GDMCC1.155, when it reached a critical concentration of 0.4 μg/mL. The results of scanning electron microscopy demonstrated that the addition of organic selenium effectively improved the morphological structure of the two bacterial cells. Molecular docking revealed that the mechanism by which organic selenium regulates QS in Lactobacillus was achieved by binding two crucial receptor proteins (histidine protein kinase HKP and periplasmic binding protein LuxP) from specific sites. Furthermore, organic selenium products have a beneficial regulatory effect on the biological activity of L. paracasei. Overall, these findings provide a new alternative (organic selenium) for regulating the viability and beneficial activity of L. paracasei.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Regulation and mechanism of organic selenium on quorum sensing, biofilm, and antioxidant effects of Lactobacillus paracasei

Lou,

Dong,

Tao

et al. 2024

Cell Biochemistry & Function

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“…It is not clear how the probiotic properties of L. plantarum are mediated by quorum sensing [25]. A recent study suggests that the enhancement of the AI-2/LuxS quorum sensing of L. plantarum enables the regulation of the interspecific bacterial communication useful to reduce and then eradicate biofilms of putrefying bacteria [26].…”

Section: Introductionmentioning

confidence: 99%

A Response Surface Methodological Approach for Large-Scale Production of Antibacterials from Lactiplantibacillus plantarum with Potential Utility against Foodborne and Orthopedic Infections

Prema,

Ali,

Nguyen

et al. 2024

Antibiotics

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A variety of bacteria, including beneficial probiotic lactobacilli, produce antibacterials to kill competing bacteria. Lactobacilli secrete antimicrobial peptides (AMPs) called bacteriocins and organic acids. In the food industry, bacteriocins, but even whole cell-free supernatants, are becoming more and more important as bio-preservatives, while, in orthopedics, bacteriocins are introducing new perspectives in biomaterials technologies for anti-infective surfaces. Studies are focusing on Lactiplantibacillus plantarum (previously known as Lactobacillus plantarum). L. plantarum exhibits great phenotypic versatility, which enhances the chances for its industrial exploitation. Importantly, more than other lactobacilli, it relies on AMPs for its antibacterial activity. In this study, Response Surface Methodology (RSM) through a Box–Behnken experimental design was used to estimate the optimal conditions for the production of antibacterials by L. plantarum. A temperature of 35 °C, pH 6.5, and an incubation time of 48 h provided the highest concentration of antibacterials. The initial pH was the main factor influencing the production of antibacterials, at 95% confidence level. Thanks to RSM, the titer of antibacterials increased more than 10-fold, this result being markedly higher than those obtained in the very few studies that have so far used similar statistical methodologies. The Box–Behnken design turned out to be a valid model to satisfactorily plan a large-scale production of antibacterials from L. plantarum.

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Metagenomic insights into quorum sensing-associated microbial profiling and its correlations with flavor compounds of Maotai-flavor liquor: A case study of stacking fermented grains

Li,

Cao,

et al. 2024

Food Research International

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Enhancing the AI-2/LuxS quorum sensing system in Lactiplantibacillus plantarum: Effect on the elimination of biofilms grown on seafoods

Cited by 6 publications

References 48 publications

Regulation and mechanism of organic selenium on quorum sensing, biofilm, and antioxidant effects of Lactobacillus paracasei

Regulation and mechanism of organic selenium on quorum sensing, biofilm, and antioxidant effects of Lactobacillus paracasei

A Response Surface Methodological Approach for Large-Scale Production of Antibacterials from Lactiplantibacillus plantarum with Potential Utility against Foodborne and Orthopedic Infections

Metagenomic insights into quorum sensing-associated microbial profiling and its correlations with flavor compounds of Maotai-flavor liquor: A case study of stacking fermented grains

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