Enhancing the concordance of two commercial dengue IgG ELISAs by exchange of the calibrator sample

Schüttoff, Tom; Adam, Awadalkareem; Reiche, Sven; Jassoy, Christian

doi:10.1016/j.jcv.2019.07.004

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…The strong correlation of the two assays indicates that the diagnostic specificity of the two tests can easily be adjusted by raising the threshold level for positive results in the NovaLisa ELISA. Methods of optimizing the cutoff have previously been described [ 6 , 18 ]. In regions where people are vaccinated against YFV, an assay with a higher threshold value is advantageous.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Specificity of Two Dengue Virus IgG ELISAs after Yellow Fever and Japanese Encephalitis Virus Vaccination

et al. 2022

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Dengue virus (DENV) antibody assays frequently cross-react with sera from individuals who have been infected with or vaccinated against related flaviviruses. The goal of this study was to determine the specificity of two DENV ELISAs with sera from individuals vaccinated against yellow fever virus (YFV) and Japanese encephalitis virus (JEV). The Panbio and the Novatec Dengue IgG ELISAs were tested with sera obtained 3–4 weeks or 0.5–6 years after YFV or JEV vaccination and the diagnostic specificity of the assays was determined. As controls, the sera were tested using DENV, YFV, JEV, Zika and West Nile virus neutralization assays. The diagnostic specificity of the Panbio and the Novatec ELISA with sera from YFV-vaccinated subjects was 98.2% and 88.2%, respectively. Cross-reactions were rare in the first 4 weeks despite high YFV-neutralizing antibody titers and were mostly found later. The specificity of the Panbio and Novatec assays with sera from JEV-vaccinated individuals was 100% and 92.9%. Cross-reactions occurred in the early time period after vaccination. The measurement values of the two ELISAs correlated strongly. Thus, the Panbio ELISA showed higher diagnostic specificity and may be suitable for seroprevalence studies in areas with high disease prevalence.

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Section: Discussionmentioning

confidence: 99%

Diagnostic Specificity of Two Dengue Virus IgG ELISAs after Yellow Fever and Japanese Encephalitis Virus Vaccination

et al. 2022

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“…All serum specimens extracted from the venous blood samples of all participants were tested on all the above reference tests from 6 December 2018 to 19 April 2019 by trained laboratory researchers blinded to the results of the index test. Commercial tests were conducted and interpreted according to the manufacturer’s instructions [ 33 , 34 , 35 ], while in-house tests were performed and interpreted as described previously [ 30 , 31 , 32 , 36 , 37 , 38 , 39 , 40 , 41 ]. ( Supplement 1 ).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the Diagnostic Accuracy of a New Biosensors-Based Rapid Diagnostic Test for the Point-Of-Care Diagnosis of Previous and Recent Dengue Infections in Malaysia

et al. 2021

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Dengue is a major threat to public health globally. While point-of-care diagnosis of acute/recent dengue is available to reduce its mortality, a lack of rapid and accurate testing for the detection of previous dengue remains a hurdle in expanding dengue seroepidemiological surveys to inform its prevention, especially vaccination, to reduce dengue morbidity. This study evaluated ViroTrack Dengue Serostate, a biosensors-based semi-quantitative anti-dengue IgG (immunoglobulin G) immuno-magnetic agglutination assay for the diagnosis of previous and recent dengue in a single test. Blood samples were obtained from 484 healthy participants recruited randomly from two communities in Petaling district, Selangor, Malaysia. The reference tests were Panbio Dengue IgG indirect and capture enzyme-linked immunosorbent assays, in-house hemagglutination inhibition assay, and focus reduction neutralization test. Dengue Serostate had a sensitivity and specificity of 91.1% (95%CI 87.8–93.8) and 91.1% (95%CI 83.8–95.8) for the diagnosis of previous dengue, and 90.2% (95%CI 76.9–97.3) and 93.2% (95%CI 90.5–95.4) for the diagnosis of recent dengue, respectively. Its positive predictive value of 97.5% (95%CI 95.3–98.8) would prevent most dengue-naïve individuals from being vaccinated. ViroTrack Dengue Serostate’s good point-of-care diagnostic accuracy can ease the conduct of dengue serosurveys to inform dengue vaccination strategy and facilitate pre-vaccination screening to ensure safety.

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West Nile Virus Seroprevalence and Cross-Neutralization in Sera from Eastern and Central Sudan

Beier

Adam

Jassoy

2022

Vector-Borne and Zoonotic Diseases

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Enhancing the concordance of two commercial dengue IgG ELISAs by exchange of the calibrator sample

Cited by 3 publications

References 8 publications

Diagnostic Specificity of Two Dengue Virus IgG ELISAs after Yellow Fever and Japanese Encephalitis Virus Vaccination

Diagnostic Specificity of Two Dengue Virus IgG ELISAs after Yellow Fever and Japanese Encephalitis Virus Vaccination

Evaluation of the Diagnostic Accuracy of a New Biosensors-Based Rapid Diagnostic Test for the Point-Of-Care Diagnosis of Previous and Recent Dengue Infections in Malaysia

West Nile Virus Seroprevalence and Cross-Neutralization in Sera from Eastern and Central Sudan

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