Enhancing the efficacy of entomopathogenic nematodes by gamma radiation in controlling Spodoptera littoralis larvae

Sayed, R. M.; Shairra, Souad

doi:10.1016/j.jrras.2016.12.005

Cited by 9 publications

(5 citation statements)

References 21 publications

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“…Research on the effect of gamma radiation on entomopathogenic nematodes showed that infective juveniles of Steinernema scapterisci Nguyen and Smart (Steinernema: Steinermatidae) and H. bacteriophora irradiated with 2 Gy gamma radiation were more effective in biocontrol of Egyptian cotton leafworm, Spodoptera littoralis Boisduval (Lepidoptera: Noctuidae), than were nonirradiated nematodes. Similar results were obtained for S. carpocapsae BA2 [169,170].…”

Section: Radiationsupporting

confidence: 87%

“…Significant deterioration in survival and infectivity [164] Radiation Wavelength of 366 nm No noticeable changes [166,167] Wavelength of 254 nm, with an exposure time of >1.75 min Reduced pathogenicity and reproduction and increased mortality [166,167] Gamma radiation Improved biological effectiveness against plant pests [169,170] Soil aeration 1-20% High survivability and density [164,173] Low oxygen content Significant increase in mortality [171,172] Metal ions Cd(II) and Pb(II)…”

Section: <4mentioning

confidence: 99%

See 1 more Smart Citation

Impact of Abiotic and Biotic Environmental Conditions on the Development and Infectivity of Entomopathogenic Nematodes in Agricultural Soils

Matuska-Łyżwa,

Duda,

Nowak

et al. 2024

Insects

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Many organisms, including beneficial entomopathogenic nematodes (EPNs), are commonly found in the soil environment. EPNs are used as biopesticides for pest control. They have many positive characteristics and are able to survive at sites of application for a long time, producing new generations of individuals. The occurrence of populations depends on many environmental parameters, such as temperature, moisture, soil texture, and pH. Extreme temperatures result in a decrease in the survival rate and infectivity of EPNs. Both high humidity and acidic soil pH reduce populations and disrupt the biological activity of EPNs. Nematodes are also exposed to anthropogenic agents, such as heavy metals, oil, gasoline, and even essential oils. These limit their ability to move in the soil, thereby reducing their chances of successfully finding a host. Commonly used fertilizers and chemical pesticides are also a challenge. They reduce the pathogenicity of EPNs and negatively affect their reproduction, which reduces the population size. Biotic factors also influence nematode biology. Fungi and competition limit the reproduction and survival of EPNs in the soil. Host availability enables survival and affects infectivity. Knowledge of the influence of environmental factors on the biology of EPNs will allow more effective use of the insecticidal capacity of these organisms.

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Section: Radiationsupporting

confidence: 87%

Section: <4mentioning

confidence: 99%

Impact of Abiotic and Biotic Environmental Conditions on the Development and Infectivity of Entomopathogenic Nematodes in Agricultural Soils

Matuska-Łyżwa,

Duda,

Nowak

et al. 2024

Insects

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“…Salem et al (2014) concluded that gamma irradiated (2Gy) S. carpocapsae may attribute as a major control method of G. mellonella. Sayed and Shairra (2017) and Sayed et al (2018) stated that 2Gy irradiated S. carpocapsae was more pathogenic than non-irradiated one against S. littoralis larvae. In addition, Sayed et al (2018) suggested that 2 Gy irradiated S. scapterisci can serve as a bio-control method for controlling B. zonata.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, Sayed et al (2015) declared that 2 Gy irradiated S. carpocapsae disrupted the antioxidant contents of S. littoralis larvae that led to larval death faster than normal S. carpocapsae. Also, Sayed and Shairra (2017) reported changes in phenoloxidase activity, lysozyme activity, and LDH activity of S. littoralis larvae treated with irradiated and non-irradiated S. scapterisci.…”

Section: Resultsmentioning

confidence: 99%

Control of Culex pipiens (Diptera: Culicidae), the vector of lymphatic filariasis, using irradiated and non-irradiated entomopathogenic nematode, Steinernema scapterisci (Rhabditida: Steinernematidae)

Sayed

Abdalla

Rizk

et al. 2018

Egypt J Biol Pest Control

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The goal of this work was to investigate the effect of gamma irradiated entomopathogenic nematode EPN; Steinernema scapterisci on third and fourth larval instars of Culex pipiens under laboratory conditions and its effect on the elemental composition of the treated larvae. Results showed that normal and irradiated tested nematodes differed in their efficacies against third and fourth larval instars. The mortality rates increased with increasing the nematode concentration and the period after treatment, in most cases the mortality in fourth instar larvae was more than in third instar larvae under the same treatments, and the percentage of mortality in irradiated nematodes was more than that in unirradiated ones. Studying the elemental composition showed that phosphorus (P) was the highest concentration in untreated larvae or those treated with normal S. scapterisci, while calcium (Ca) was the highest concentration in gamma irradiated S. scapterisci. The lowest concentration in untreated larvae was zinc (Zn), while copper (Cu) was the lowest concentration in unirradiated and 2 Gy gamma irradiated S. scapterisci. In conclusion, the gamma irradiation of S. scapterisci enhanced its efficacy against C. pipiens, therefore it could serve as safe control method for C. pipiens.

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“…They found that, the efficiency was enhanced against G. mellonella. (Sayed, 2011) and Spodoptera littoralis (Sayed and Shairra, 2017).…”

Section: Discussionmentioning

confidence: 99%

Effect of Gamma Irradiation on Viability, Pathogenicity, Reproductivity of Heterorhabditis bacteriophora and H. indica and detection of DNA Damage

Taha

Shoaib

Abo-Shady

2021

Journal of Plant Protection and Pathology

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Entomopathogenic nematodes (EPNs) are a promising alternative method for insect pest control. The idea of the work is to investigate the effect of gamma irradiation on EPNs pathogenicity under laboratory conditions aiming to enhance their lethal effect against insect pests. Heterorhabditis bacteriophora (Hb) as well as H. indica (Hi) were irradiated directly in nematode suspension and indirectly within the insect cadavers by gamma irradiation dosages of 0, 100, 250, 500, 750 and 1000 Gy. The mortality percentage of nematodes were recorded, the extracted nematodes from the irradiated insect hosts were used to investigate their pathogenicity and reproductivity against Galleria mellonella larvae. The damage of DNA was analyzed using the comet assay and the LC50 of the two nematode species was recorded. The data demonstrated that, the low dosage (100 Gy) enhanced the pathogenicity of EPNs and didn't affect the initial populations (Pi), the final population of emerged IJs (Pf) and the rate of reproduction (Rr%). Moreover, H. bacteriophora was more tolerant to gamma irradiation than H. indica which recorded more DNA damage %, DNA in tail, tail length, tail moment and olive tail moment in the all different irradiated dosages. In conclusion, low dosages of gamma irradiation can be used to enhance the pathogenicity and reproductivity of entomopathogenic nematodes in order to use as a biocontrol agent.

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Enhancing the efficacy of entomopathogenic nematodes by gamma radiation in controlling Spodoptera littoralis larvae

Cited by 9 publications

References 21 publications

Impact of Abiotic and Biotic Environmental Conditions on the Development and Infectivity of Entomopathogenic Nematodes in Agricultural Soils

Impact of Abiotic and Biotic Environmental Conditions on the Development and Infectivity of Entomopathogenic Nematodes in Agricultural Soils

Control of Culex pipiens (Diptera: Culicidae), the vector of lymphatic filariasis, using irradiated and non-irradiated entomopathogenic nematode, Steinernema scapterisci (Rhabditida: Steinernematidae)

Effect of Gamma Irradiation on Viability, Pathogenicity, Reproductivity of Heterorhabditis bacteriophora and H. indica and detection of DNA Damage

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