Enhancing the efficiency of lung cancer cell capture using microfluidic dielectrophoresis and aptamer‐based surface modification

Lin, Shu‐Hui; Su, Tzu‐Cheng; Huang, Shuo Jie; Jen, Chun‐Ping

doi:10.1002/elps.202300206

Cited by 2 publications

(1 citation statement)

References 35 publications

(52 reference statements)

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“…For example, it can be applied to the design of point-of-care (POC) devices for timely diagnosis and treatment for patients [13,14]. It can also be potentially helpful especially in developing LOC devices to mimic human vascular networks, and blood-air barriers in the human respiratory system to investigate lung disease progression, diagnosis, and treatment [8,12,[14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Analytical Solution for Transient Electroosmotic and Pressure-Driven Flows in Microtubes

Feng,

Yi,

Liu

2024

Fluids

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This study focuses on deriving and presenting an infinite series as the analytical solution for transient electroosmotic and pressure-driven flows in microtubes. Such a mathematical presentation of fluid dynamics under simultaneous electric field and pressure gradients leverages governing equations derived from the generalized continuity and momentum equations simplified for laminar and axisymmetric flow. Velocity profile developments, apparent slip-induced flow rates, and shear stress distributions were analyzed by varying values of the ratio of microtube radius to Debye length and the electroosmotic slip velocity. Additionally, the “retarded time” in terms of hydraulic diameter, kinematic viscosity, and slip-induced flow rate was derived. A simpler polynomial series approximation for steady electroosmotic flow is also proposed for engineering convenience. The analytical solutions obtained in this study not only enhance the fundamental understanding of the electroosmotic flow characteristics within microtubes, emphasizing the interplay between electroosmotic and pressure-driven mechanisms, but also serve as a benchmark for validating computational fluid dynamics models for electroosmotic flow simulations in more complex flow domains. Moreover, the analytical approach aids in the parametric analysis, providing deeper insights into the impact of physical parameters on electroosmotic and pressure-driven flow behavior, which is critical for optimizing device performance in practical applications. These findings also offer insightful implications for diagnostic and therapeutic strategies in healthcare, particularly enhancing the capabilities of lab-on-a-chip technologies and paving the way for future research in the development and optimization of microfluidic systems.

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Section: Introductionmentioning

confidence: 99%

Analytical Solution for Transient Electroosmotic and Pressure-Driven Flows in Microtubes

Feng,

Yi,

Liu

2024

Fluids

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Separation of Lung Cancer Cells From Mixed Cell Samples Using Aptamer‐Modified Magnetic Beads and Permalloy Micromagnets

Lin,

Tsai,

et al. 2024

Electrophoresis

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This study involved the design and fabrication of a microfluidic chip integrated with permalloy micromagnets. The device was used with aptamer‐modified magnetic beads (MBs) of various sizes to successfully separate lung cancer cells from a mixture of other cells. The overall separation efficiency was evaluated based on the ratios of cells in the different outlets and inlets of the chip. The results showed efficiencies ranging from 43.4% to 50.2% for MB sizes between 1.36 and 4.50 µm. Interestingly, efficiency slightly decreased as the size of the MBs increased, contrary to predictions. Further examination revealed that larger MBs exerted gravitational force on the cell‐bound MBs at low flow rates, causing the targets to settle before reaching the main microchannel region. This was attributed to fluidic resistance caused by a size mismatch between the inlet tube and the microfluidic conduit. An increase in cell accumulation at the inlet was observed with larger MB sizes due to gravity. Therefore, the definition of effective separation efficiency was revised to exclude the effect of cell accumulation at the inlet. Effective separation efficiencies were found to be 71.6%, 76.4%, and 79.4% for MB sizes of 1.36, 3.00, and 4.50 µm, respectively. The study concluded that larger MBs interacted more with the magnetic force, resulting in better separation. However, cells with smaller MBs were more likely to evade the magnetic force. The investigation provides valuable insights into isolating lung cancer cells using this method, with the potential for clinical application in cancer diagnosis and treatment.

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Enhancing the efficiency of lung cancer cell capture using microfluidic dielectrophoresis and aptamer‐based surface modification

Cited by 2 publications

References 35 publications

Analytical Solution for Transient Electroosmotic and Pressure-Driven Flows in Microtubes

Analytical Solution for Transient Electroosmotic and Pressure-Driven Flows in Microtubes

Separation of Lung Cancer Cells From Mixed Cell Samples Using Aptamer‐Modified Magnetic Beads and Permalloy Micromagnets

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