Enhancing the growth of Physcomitrella patens by combination of monochromatic red and blue light – a kinetic study

Abstract

In the current work we demonstrate the relevance of monochromatic light conditions in moss plant cell culture. Light intensity and illumination wavelength are important cultivation parameters due to their impact on growth and chlorophyll formation kinetics of the moss Physcomitrella patens. This moss was chosen as a model organism due to its capability to produce complex recombinant pharmaceutical proteins. Filamentous moss cells were cultivated in mineral medium in shaking flasks. The flasks were illuminated by light emitting diodes (LED) providing nearly monochromatic red and blue light as well as white light as a reference. A maximum growth rate of 0.78 day((1) was achieved under additional CO(2) aeration and no growth inhibition was observed under high light illumination. The application of dual red and blue light is the most effective way to reach high growth and chlorophyll formation rates while minimizing energy consumption of the LEDs. These observations are discussed as effects of photo sensory pigments in the moss. The combination of monochromatic red and blue light should be considered when a large scale process is set up.

Moss‐made pharmaceuticals: from bench to bedside

“…As in algae, the dominant phase of moss development is haploid (gametophytic), while the dominant phase in all seed plants is at least diploid (sporophytic). Mosses can be grown in self‐contained systems like Petri dishes, Erlenmeyer flasks or bioreactors in pure mineral media without any organic additions such as antibiotics, carbon sources or growth regulators and under highly controlled environmental conditions (Cerff and Posten, ; Hohe and Reski, ; Hohe et al ., ). Even under these conditions, the moss P. patens can complete its life cycle with the release of persistent spores.…”

Photobioreactors in Life Support Systems

“…Depending on the light color that is used, different photoconversion efficiencies (PCE) will result. One work concerning LED illumination with different colors is described by Cerff et al [32] concerning the growth of the moss Physcomitrella patens as a representative of plants. Several studies have also been published on the influence of light colors on the growth of microalgae which is discussed in the following chapter.…”

“…Their long lifetime (about one year in continuous PBR operation) [74] especially makes them the best choice among different light sources. Concerning internal efficiency of LEDs the color chosen is also important inasmuch as red LEDs, for example, need 30 % less energy than blue or white LEDs [32].…”

Editorial: Enhancing productivity of CHO, plant and algal cells

“…Much research is being conducted to improve culture conditions and optimize photobiorectors (see recent examples in [4][5][6][7][8]). In this issue, Martin Cerff and Clemens Posten optimize the growth of the moss Physcomitrella patens by a combination of monochromatic red and blue light [9]. Modeling of microalgae cultivation growth and productivities in different geographic regions with varying solar irradiation and temperatures profiles is You may have noticed that this issue of Biotechnology Journal does not feature a particular theme, as we have done for the majority of our issues to date [1].…”