Enhancing the Intestinal Permeation of the Chondroprotective Nutraceuticals Glucosamine Sulphate and Chondroitin Sulphate Using Conventional and Modified Liposomes

Agiba, Ahmed M.; Nasr, Maha; Abdel-Hamid, Sameh; Eldin, Ahmed Badr; Geneidi, Ahmed S.

doi:10.2174/1567201815666180123100148

Cited by 38 publications

(13 citation statements)

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“…Briefly, the chromatographic conditions were C18 column (Hypersil, Thermo Scientific), mobile phase methanol:water containing 0.5% acetic acid 94:6, and a detection wavelength 279 nm. The encapsulated drug was computed as follows 30,31 :…”

Section: Methodsmentioning

confidence: 99%

Polymeric nanocapsular baicalin: Chemometric optimization, physicochemical characterization and mechanistic anticancer approaches on breast cancer cell lines

El-Gogary

Gaber

Nasr

2019

Sci Rep

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Baicalin is a multi-purpose flavonoid known for its anticancer properties, but its application is hindered by its low water solubility and bioavailability. Polymeric nanocapsules were proposed in this work as a promising system for enhancing baicalin delivery, and potentiating its anticancer properties. The characterization of nanocapsules was augmented with chemometric analysis, and the selected formulations were tested on two breast cancer cell lines (MCF-7 and MDA-MB-231), with mechanistic anticancer elucidation using MTT assay, confocal microscopy uptake, flow cytometry, mechanism of cell death, reactive oxygen species production, caspase 3/7 activity and death biomarker expression using quantitative real time PCR. Results showed that baicalin nanocapsules displayed favorable pharmaceutical properties; with the formulation variables affecting their properties elucidated using chemometric factorial analysis. Nanocapsules enhanced the anticancer activity of baicalin up to 216 times for MCF-7 cells and 31 times for MDA-MB-231 after 24 hr incubation. Cellular internalization of the fluorescently labeled nanocapsules was confirmed after 4 hr incubation for both cell lines. Apoptosis was the dominant cell death mechanism, with significant up-regulation of P53 in baicalin nanocapsules treated cells. Data here presented drive to further preclinical studies to investigate the delivery of baicalin polymeric nanocapsules and their anti-cancer activity.

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Section: Methodsmentioning

confidence: 99%

Polymeric nanocapsular baicalin: Chemometric optimization, physicochemical characterization and mechanistic anticancer approaches on breast cancer cell lines

El-Gogary

Gaber

Nasr

2019

Sci Rep

Self Cite

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“…CS being a negatively charged polymer with a molecular weight ranging from 15 to 50 kDa cannot diffuse passively through the skin layers because of its negative charge, hydrophilicity, and size and therefore needs active administration. In order to overcome this challenge, there are several efforts toward encapsulating CS in liposomes, scaffolds, microspheres, or gold nanoparticles ,, for delivery to the bone and other organs. However, topical administration of CS to skin in a noninvasive manner has not been attempted.…”

Section: Discussionmentioning

confidence: 99%

“…CS is more sulfated than HA, which makes it even more difficult to deliver it to or through skin. In the case of osteoarthritis, CS is replenished primarily through oral or intraarticular administrations, while few attempts of topical administration have also been made. , There are isolated examples in the literature of different drug delivery systems made of polymers such as poly(lactic- co -glycolic acid), gelatin, gold nanoparticles, and liposomal formulations in conjunction with permeation enhancers primarily for delivery of CS to bones. − However, delivery of CS to skin through topical administration is elusive.…”

Section: Introductionmentioning

confidence: 99%

Topical Application of Peptide–Chondroitin Sulfate Nanoparticles Allows Efficient Photoprotection in Skin

Mishra

Pal

et al. 2021

ACS Appl. Mater. Interfaces

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In this article, we describe a method of delivery of chondroitin sulfate to skin as nanoparticles and demonstrate its anti-inflammatory and antioxidant role using UV irradiation as a model condition. These nanoparticles, formed through electrostatic interactions of chondroitin sulfate with a skin-penetrating peptide, were found to be homogenous with positive surface charges and stable at physiological and acidic pH under certain conditions. They were able to enter into the human keratinocyte cell line (HaCaT), artificial skin membrane (mimicking the human skin), and mouse skin tissue unlike free chondroitin sulfate. The preapplication of nanoparticles also exhibited reduced levels of oxidative stress, cyclobutane pyrimidine dimer formation, TNF-α, and so on in UV-B-irradiated HaCaT cells. In an acute UV-B irradiation mouse model, their topical application resulted in reduced epidermal thickness and sunburn cells, unlike in the case of free chondroitin sulfate. Thus, a completely noninvasive method was used to deliver a bio-macromolecule into the skin without using injections or abrasive procedures.

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“…Thus, this work extended our knowledge on apatinib, and injection of Apa-MS into HCC tissues of patients guided by CT or digital subtraction angiography would be a promising combination strategy to enhance the effect of RFA. 37 …”

Section: Discussionmentioning

confidence: 99%

A new apatinib microcrystal formulation enhances the effect of radiofrequency ablation treatment on hepatocellular carcinoma

Xie¹,

Tian²,

Yu³

et al. 2018

OTT

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IntroductionRadiofrequency ablation (RFA) is the foremost treatment option for advanced hepatocellular carcinoma (HCC), however, rapid and aggressive recurrence of HCC often occurs after RFA due to epithelial–mesenchymal transition process. Although combination of RFA with sorafenib, a molecular targeted agent, could attenuate the recurrence of HCC, application of this molecular targeted agent poses a heavy medical burden and oral administration of sorafenib also brings severe side effects.Materials and methodsIn this study, we prepared an apatinib microcrystal formulation (Apa-MS) that sustainably releases apatinib, a novel molecular targeted agent, for advanced HCC treatment. We injected apatinib solution or Apa-MS into subcutaneous HCC tumors.ResultsIt was found that Apa-MS exhibited slow apatinib release in vivo and in turn inhibited the epithelial–mesenchymal transition of HCC cells for extended time. Moreover, in rodent HCC model, Apa-MS enhanced the antitumor effect of RFA treatment.ConclusionBased on these results, we conclude that Apa-MS, a slow releasing system of apatinib, allows apatinib to remain effective in tumor tissues for a long time and could enhance the antitumor effect of RFA on HCC.

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Enhancing the Intestinal Permeation of the Chondroprotective Nutraceuticals Glucosamine Sulphate and Chondroitin Sulphate Using Conventional and Modified Liposomes

Cited by 38 publications

References 0 publications

Polymeric nanocapsular baicalin: Chemometric optimization, physicochemical characterization and mechanistic anticancer approaches on breast cancer cell lines

Polymeric nanocapsular baicalin: Chemometric optimization, physicochemical characterization and mechanistic anticancer approaches on breast cancer cell lines

Topical Application of Peptide–Chondroitin Sulfate Nanoparticles Allows Efficient Photoprotection in Skin

A new apatinib microcrystal formulation enhances the effect of radiofrequency ablation treatment on hepatocellular carcinoma

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