Enhancing the lipid content of Scenedesmus obtusiusculus AT-UAM by controlled acidification under indoor and outdoor conditions

Sánchez-García, León; Cabello, Juan J.; Jiménez‐García, Luis Felipe; Revah, Sergio; Morales-Ibarría, Marcia

doi:10.1016/j.algal.2020.102024

Cited by 15 publications

(18 citation statements)

References 39 publications

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“…Table 2 compares different strategies to enhance lipid and carbohydrate productivity by different microalgae. In this context, authors have implemented different strategies to increase lipid accumulation in microalgae using N deprivation, different sources and concentrations of N, micronutrient limitation using Ca and Mg, controlled acidification, temperature, photoperiod and light intensity 5,8,14,15,41,42 . However, stress conditions reduce biomass growth rates, directly affecting lipid productivity.…”

Section: Resultsmentioning

confidence: 99%

“…Biomass productivity ( P b ) (mg L −1 d −1 ) for batch cultivation was calculated using Eqn (1), 16 where C 0 is the initial biomass concentration as TSS (mg L −1 ) in the culture at time t 0 (days), and C f is the final biomass concentration (mg L −1 ) of the culture at time t f (days):

P_{b} goodbreak= \frac{C_{f} - C_{0}}{t_{f} - t_{0}}

The average biomass productivity was calculated using Eqn (2) for semi‐continuous 25 cultivation, where C i ,0 (mg L −1 ) is the initial biomass concentration after mineral medium renewal, i , at time t i ,0 (days), and C i ,f (mg L −1 ) is the final biomass concentration of i renewing at time t i ,f (days) under light exposure; n is the total number of partial liquid replacements completed and sc is semi‐continuous operation:

P_{normalb_sc} goodbreak= \frac{[\sum_{i = 0}^{n} (\frac{C_{i, normalf} - C_{i, 0}}{t_{i, normalf} - t_{i, 0}})]}{n} .

In a similar way, the average lipid and carbohydrate productivity ( P l_sc and P CH_sc , respectively) (mg L −1 d −1 ) were determined using Eqns (3) and (4), 8 where P b (mg L −1 d −1 ) is the biomass productivity, and X l and X CH (% DCW) is the final lipid and carbohydrate content per milligram of biomass, respectively:

P_{l} goodbreak= P_{b} goodbreak\times X_{l} P_{normall_sc} goodbreak= \frac{[\sum_{i = 0}^{n} (P_{l, i} X_{l, i})]}{n},

P_{CH} goodbreak= P_{b} goodbreak\times X_{CH} P_{CH_sc} goodbreak= <...>

…”

Section: Methodsmentioning

confidence: 99%

“…Biodiesel can be produced by transesterification of microalgal lipids, since the oil production from microalgae is approximately 10–300 times higher than that in plants 12 . Different stress conditions have been explored to trigger lipid accumulation in microalgae, such as nutrient limitation (nitrogen (N) or phosphate starvation), 12,13 pH shock 8,14 or increased salinity 15 …”

Section: Introductionmentioning

confidence: 99%

“…Previous research performed with the oleaginous microalga Scenedesmus obtusiusculus AT‐UAM demonstrated its biotechnological potential to produce oils and capture CO 2 simultaneously. Scenedesmus obtusiusculus AT‐UAM accumulated lipids up to about 56% dry cell weight (DCW), with a productivity of 85 mg L −1 d −1 under N starvation in batch cultivation 8 . It also captured CO 2 at a rate of 950 mg L −1 d −1 in N‐sufficient medium 13,16 .…”

Section: Introductionmentioning

confidence: 99%

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Effect of nitrogen feast–famine cycles and semi‐continuous cultivation on the productivity of energy‐rich compounds by Scenedesmus obtusiusculus AT‐UAM

Gorry

Ángeles

Revah³

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND Nitrogen (N) limitation is the most common strategy used to accumulate energy‐rich compounds in microalgae. However, this condition reduces the microalga growth rate and biomass, lipid and carbohydrate productivities. In this work, a semi‐continuous operation strategy combined with N feast–famine cycles were investigated in order to increase the lipid, carbohydrate and biomass contents in the microalga Scenedesmus obtusiusculus AT‐UAM. RESULTS Growth and lipid productivity were evaluated in a flat‐panel photobioreactor (FP‐PBR) under controlled conditions, through 72 and 24 h N feast–famine cycles at different N concentrations (6, 12 and 24 mg N L−1). The highest accumulation of microalgal biomass, lipid and carbohydrate (550 ± 28, 139 ± 4 and 198 ± 7 mg L−1 d−1) was obtained for 24 h cycles at 12 mg N L−1 and were up to ~2.4 times compared to that of 72 h N feast–famine cycles. This strategy was also applied in a bubble column photobioreactor (BC‐PBR) located in a greenhouse. Here, biomass productivities obtained under a semi‐continuous operation for 24 h N feast–famine cycles with 6 and 12 mg N L−1 were 536 ± 27 and 667 ± 33 mg L−1 d−1, respectively. The maximum productivity of lipid and carbohydrate reached 142 ± 10 and 169 ± 14 mg L−1 d−1 with 12 mg N L−1, respectively. CONCLUSION The studied N feast–famine cycles and semi‐continuous operations increased the productivity of biomass and lipids in S. obtusiusculus AT‐UAM. © 2021 Society of Chemical Industry (SCI).

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Section: Resultsmentioning

confidence: 99%

P_{b} goodbreak= \frac{C_{f} - C_{0}}{t_{f} - t_{0}}

P_{normalb_sc} goodbreak= \frac{[\sum_{i = 0}^{n} (\frac{C_{i, normalf} - C_{i, 0}}{t_{i, normalf} - t_{i, 0}})]}{n} .

P_{l} goodbreak= P_{b} goodbreak\times X_{l} P_{normall_sc} goodbreak= \frac{[\sum_{i = 0}^{n} (P_{l, i} X_{l, i})]}{n},

P_{CH} goodbreak= P_{b} goodbreak\times X_{CH} P_{CH_sc} goodbreak= <...>

…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of nitrogen feast–famine cycles and semi‐continuous cultivation on the productivity of energy‐rich compounds by Scenedesmus obtusiusculus AT‐UAM

Gorry

Ángeles

Revah³

et al. 2021

J of Chemical Tech & Biotech

Self Cite

View full text Add to dashboard Cite

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“…2. Algae will cycle these biochemically active compounds within cells to meet changing physiological needs (Safafar et al 2016;Sánchez-García et al 2020). NO 3 − is the most common N compound used in microalgae cultivation media and is taken up by active mechanisms (Richmond 2008;Kim et al 2013).…”

Section: Phycoremediation In the Treatment Of Excess Nutrientsmentioning

confidence: 99%

Advances in POME treatment methods: potentials of phycoremediation, with a focus on South East Asia

Tan

Maznah

Morad

et al. 2021

Int. J. Environ. Sci. Technol.

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The discharge of palm oil mill effluent (POME) without effective treatment is highly toxic to the environment and receiving waters due to its high chemical oxygen demand , biochemical oxygen demand and increased levels of suspended solids. The ponding system is regarded as a conventional method for the treatment of POME but requires long retention time and extensive treatment areas. Other processes, such as physicochemical treatment, have also been applied as tertiary steps to ensure lower toxicity of the POME discharge. More recently, phycoremediation, a new biological treatment method using microalgae, has been reported as having great potential in being a highly efficient method of reducing the environmental impact of POME. This paper discusses the feasibility of the above treatment methods of POME, including the potential of phycoremediation, with a focus on their application in South East Asia.

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Microstructure Engineered Photon‐Managing Films for Solar Energy to Biomass Conversion

Shen

Lou

Aili

et al. 2023

Advanced Energy Materials

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such as solar thermal collectors, [2] photovoltaic cells, [3] photocatalytic hydrogen production, [4] and photosynthesis. [5] Among them, photosynthesis is considered a sustainable process by which photosynthetic organisms convert solar light energy into chemical energy (biomass) using water and CO 2 . For this reason, photosynthesis has gained the increasingly concern of the researchers for applications in CO 2 biofixation [6] and the production of sustainable bioresources. [7] Compared with higher plants, microalgae show advantages in solar energy conversion through photosynthesis due to their fast growth rates and their ability to grow on non-arable land using saline water. [8] Nonetheless, photosynthetic efficiencies of microalgae are much lower than their theoretical maxima, which is predominantly attributed to the inefficient utilization of solar light reaching the microalgae. [9] To improve light utilization efficiency, genetic engineering has been explored. [10] It accelerates light-dependent reactions of microalgae and ultimately augments the photosynthesis by artificially tailoring their light-harvesting antenna size [9b] and expanding the photosynthetically active radiation (PAR) spectrum. [10a] Nevertheless, only limited microalgal strains nowadays can be genetically manipulated, [11] for example, Chlamydomonas reinhardtii (C. reinhardtii), and much effort is still needed to understand the stability and reliability of the genetically modified microalgae and other algal strains. [12] Photon management, on the other hand, provides a passive approach to increasing the photosynthesis of microalgae by Conversion of solar energy into chemical energy through natural photosynthesis plays a crucial role in sustainable energy transformation, bioresource production, and CO 2 biofixation. Nevertheless, the overall solar energy to chemical energy (biomass) conversion efficiency in the photosynthetic organisms is still unsatisfactory because of their inefficient utilization of solar light. Here, a photonic method to improve photosynthesis of a unicellular green microalga, Chlamydomonas reinhardtii, a photosynthetic organism model is reported. For this purpose, an easy-to-fabricate microphotonic film is developed to improve the spectral quality of solar light reaching the microalgae through photon management (i.e., simultaneous solar spectral conversion and directional fluorescent emission). This study demonstrates that the short-term oxygen evolution rate and the long-duration biomass production of microalgae in 200-mL laboratory photobioreactors are enhanced by a factor of 38% and 54%, respectively. In 5000-mL scaled-up bubble column photobioreactors placed outdoor under natural sunlight and weather conditions, the biomass yield is improved by more than 20% when compared to the control experiments conducted in parallel in an optically clear bubble column photobioreactor. Based on such experimental observations, the work here demonstrates the potential of photon management for promoting the solar energy...

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Enhancing the lipid content of Scenedesmus obtusiusculus AT-UAM by controlled acidification under indoor and outdoor conditions

Cited by 15 publications

References 39 publications

Effect of nitrogen feast–famine cycles and semi‐continuous cultivation on the productivity of energy‐rich compounds by Scenedesmus obtusiusculus AT‐UAM

Effect of nitrogen feast–famine cycles and semi‐continuous cultivation on the productivity of energy‐rich compounds by Scenedesmus obtusiusculus AT‐UAM

Advances in POME treatment methods: potentials of phycoremediation, with a focus on South East Asia

Microstructure Engineered Photon‐Managing Films for Solar Energy to Biomass Conversion

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